Abstract
We describe here a simple method for analyzing the stability of recombinant yeast. The mouse salivary α-amylase gene was used in this system as a marker since the stability of recombinant yeast can be detected easily by a halo zone-forming assay on starch-supplemented plates. We used this method to evaluate the stability of recombinant yeast harbouring a 2μ directed episomal plasmid and of yeast harbouring the r-DNA directed integrative vector. Our results demonstrated clearly that the α-amylase gene was a convenient and reliable marker for evaluating the stability of recombinant yeasts in long-term cultivation.
Original language | English |
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Pages (from-to) | 329-334 |
Number of pages | 6 |
Journal | Journal of Biotechnology |
Volume | 29 |
Issue number | 3 |
DOIs | |
Publication status | Published - 1993 Jun |
All Science Journal Classification (ASJC) codes
- Biotechnology
- Bioengineering
- Applied Microbiology and Biotechnology