α-Amylase as a marker for evaluating the stability of recombinant yeast in long-term cultivation

Dz Chi Chen, Bei-Chang Yang, Chi Wan Chow, Tsong Teh Kuo

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

We describe here a simple method for analyzing the stability of recombinant yeast. The mouse salivary α-amylase gene was used in this system as a marker since the stability of recombinant yeast can be detected easily by a halo zone-forming assay on starch-supplemented plates. We used this method to evaluate the stability of recombinant yeast harbouring a 2μ directed episomal plasmid and of yeast harbouring the r-DNA directed integrative vector. Our results demonstrated clearly that the α-amylase gene was a convenient and reliable marker for evaluating the stability of recombinant yeasts in long-term cultivation.

Original languageEnglish
Pages (from-to)329-334
Number of pages6
JournalJournal of Biotechnology
Volume29
Issue number3
DOIs
Publication statusPublished - 1993 Jan 1

Fingerprint

Amylases
Yeast
Yeasts
Genes
Starch
Assays
Plasmids
DNA

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

Cite this

@article{ed21ad27e8604cd984b1daa22e286c53,
title = "α-Amylase as a marker for evaluating the stability of recombinant yeast in long-term cultivation",
abstract = "We describe here a simple method for analyzing the stability of recombinant yeast. The mouse salivary α-amylase gene was used in this system as a marker since the stability of recombinant yeast can be detected easily by a halo zone-forming assay on starch-supplemented plates. We used this method to evaluate the stability of recombinant yeast harbouring a 2μ directed episomal plasmid and of yeast harbouring the r-DNA directed integrative vector. Our results demonstrated clearly that the α-amylase gene was a convenient and reliable marker for evaluating the stability of recombinant yeasts in long-term cultivation.",
author = "Chen, {Dz Chi} and Bei-Chang Yang and Chow, {Chi Wan} and Kuo, {Tsong Teh}",
year = "1993",
month = "1",
day = "1",
doi = "10.1016/0168-1656(93)90064-T",
language = "English",
volume = "29",
pages = "329--334",
journal = "Journal of Biotechnology",
issn = "0168-1656",
publisher = "Elsevier",
number = "3",

}

α-Amylase as a marker for evaluating the stability of recombinant yeast in long-term cultivation. / Chen, Dz Chi; Yang, Bei-Chang; Chow, Chi Wan; Kuo, Tsong Teh.

In: Journal of Biotechnology, Vol. 29, No. 3, 01.01.1993, p. 329-334.

Research output: Contribution to journalArticle

TY - JOUR

T1 - α-Amylase as a marker for evaluating the stability of recombinant yeast in long-term cultivation

AU - Chen, Dz Chi

AU - Yang, Bei-Chang

AU - Chow, Chi Wan

AU - Kuo, Tsong Teh

PY - 1993/1/1

Y1 - 1993/1/1

N2 - We describe here a simple method for analyzing the stability of recombinant yeast. The mouse salivary α-amylase gene was used in this system as a marker since the stability of recombinant yeast can be detected easily by a halo zone-forming assay on starch-supplemented plates. We used this method to evaluate the stability of recombinant yeast harbouring a 2μ directed episomal plasmid and of yeast harbouring the r-DNA directed integrative vector. Our results demonstrated clearly that the α-amylase gene was a convenient and reliable marker for evaluating the stability of recombinant yeasts in long-term cultivation.

AB - We describe here a simple method for analyzing the stability of recombinant yeast. The mouse salivary α-amylase gene was used in this system as a marker since the stability of recombinant yeast can be detected easily by a halo zone-forming assay on starch-supplemented plates. We used this method to evaluate the stability of recombinant yeast harbouring a 2μ directed episomal plasmid and of yeast harbouring the r-DNA directed integrative vector. Our results demonstrated clearly that the α-amylase gene was a convenient and reliable marker for evaluating the stability of recombinant yeasts in long-term cultivation.

UR - http://www.scopus.com/inward/record.url?scp=0027316318&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0027316318&partnerID=8YFLogxK

U2 - 10.1016/0168-1656(93)90064-T

DO - 10.1016/0168-1656(93)90064-T

M3 - Article

VL - 29

SP - 329

EP - 334

JO - Journal of Biotechnology

JF - Journal of Biotechnology

SN - 0168-1656

IS - 3

ER -