α-Amylase as a marker for evaluating the stability of recombinant yeast in long-term cultivation

Dz Chi Chen, Bei Chang Yang, Chi Wan Chow, Tsong Teh Kuo

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

We describe here a simple method for analyzing the stability of recombinant yeast. The mouse salivary α-amylase gene was used in this system as a marker since the stability of recombinant yeast can be detected easily by a halo zone-forming assay on starch-supplemented plates. We used this method to evaluate the stability of recombinant yeast harbouring a 2μ directed episomal plasmid and of yeast harbouring the r-DNA directed integrative vector. Our results demonstrated clearly that the α-amylase gene was a convenient and reliable marker for evaluating the stability of recombinant yeasts in long-term cultivation.

Original languageEnglish
Pages (from-to)329-334
Number of pages6
JournalJournal of Biotechnology
Volume29
Issue number3
DOIs
Publication statusPublished - 1993 Jun

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

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