α-Amylase as a marker for evaluating the stability of recombinant yeast in long-term cultivation

Dz Chi Chen; Bei Chang Yang; Chi Wan Chow; Tsong Teh Kuo

doi:10.1016/0168-1656(93)90064-T

α-Amylase as a marker for evaluating the stability of recombinant yeast in long-term cultivation

Dz Chi Chen
, Bei Chang Yang
, Chi Wan Chow
, Tsong Teh Kuo

Department of Microbiology and Immunology

Research output: Contribution to journal › Article › peer-review

5 Citations (Scopus)

Abstract

We describe here a simple method for analyzing the stability of recombinant yeast. The mouse salivary α-amylase gene was used in this system as a marker since the stability of recombinant yeast can be detected easily by a halo zone-forming assay on starch-supplemented plates. We used this method to evaluate the stability of recombinant yeast harbouring a 2μ directed episomal plasmid and of yeast harbouring the r-DNA directed integrative vector. Our results demonstrated clearly that the α-amylase gene was a convenient and reliable marker for evaluating the stability of recombinant yeasts in long-term cultivation.

Original language	English
Pages (from-to)	329-334
Number of pages	6
Journal	Journal of Biotechnology
Volume	29
Issue number	3
DOIs	https://doi.org/10.1016/0168-1656(93)90064-T
Publication status	Published - 1993 Jun

All Science Journal Classification (ASJC) codes

Biotechnology
Bioengineering
Applied Microbiology and Biotechnology

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10.1016/0168-1656(93)90064-T

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title = "α-Amylase as a marker for evaluating the stability of recombinant yeast in long-term cultivation",

abstract = "We describe here a simple method for analyzing the stability of recombinant yeast. The mouse salivary α-amylase gene was used in this system as a marker since the stability of recombinant yeast can be detected easily by a halo zone-forming assay on starch-supplemented plates. We used this method to evaluate the stability of recombinant yeast harbouring a 2μ directed episomal plasmid and of yeast harbouring the r-DNA directed integrative vector. Our results demonstrated clearly that the α-amylase gene was a convenient and reliable marker for evaluating the stability of recombinant yeasts in long-term cultivation.",

author = "Chen, \{Dz Chi\} and Yang, \{Bei Chang\} and Chow, \{Chi Wan\} and Kuo, \{Tsong Teh\}",

note = "Funding Information: Thanks are due to Drs J.R. Mattoon and R. Wu for kindly giving us the plasmids DNA. This work was supported by the National Science Council, Taiwan.",

year = "1993",

month = jun,

doi = "10.1016/0168-1656(93)90064-T",

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T1 - α-Amylase as a marker for evaluating the stability of recombinant yeast in long-term cultivation

AU - Chen, Dz Chi

AU - Yang, Bei Chang

AU - Chow, Chi Wan

AU - Kuo, Tsong Teh

N1 - Funding Information: Thanks are due to Drs J.R. Mattoon and R. Wu for kindly giving us the plasmids DNA. This work was supported by the National Science Council, Taiwan.

PY - 1993/6

Y1 - 1993/6

N2 - We describe here a simple method for analyzing the stability of recombinant yeast. The mouse salivary α-amylase gene was used in this system as a marker since the stability of recombinant yeast can be detected easily by a halo zone-forming assay on starch-supplemented plates. We used this method to evaluate the stability of recombinant yeast harbouring a 2μ directed episomal plasmid and of yeast harbouring the r-DNA directed integrative vector. Our results demonstrated clearly that the α-amylase gene was a convenient and reliable marker for evaluating the stability of recombinant yeasts in long-term cultivation.

AB - We describe here a simple method for analyzing the stability of recombinant yeast. The mouse salivary α-amylase gene was used in this system as a marker since the stability of recombinant yeast can be detected easily by a halo zone-forming assay on starch-supplemented plates. We used this method to evaluate the stability of recombinant yeast harbouring a 2μ directed episomal plasmid and of yeast harbouring the r-DNA directed integrative vector. Our results demonstrated clearly that the α-amylase gene was a convenient and reliable marker for evaluating the stability of recombinant yeasts in long-term cultivation.

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M3 - Article

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AN - SCOPUS:0027316318

SN - 0168-1656

VL - 29

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EP - 334

JO - Journal of Biotechnology

JF - Journal of Biotechnology

IS - 3

ER -

α-Amylase as a marker for evaluating the stability of recombinant yeast in long-term cultivation

Abstract

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