α1-antitrypsin precursor in gastric juice is a novel biomarker for gastric cancer and ulcer

Ping I. Hsu, Chung Hsuan Chen, Chieu Shia Hsieh, Wei Chao Chang, Kwok Hung Lai, Gin Ho Lo, Ping Ning Hsu, Feng Woei Tsay, Yu Shan Chen, Michael Hsiao, Hui Chun Chen, Pei-Jung Lu

Research output: Contribution to journalArticle

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Abstract

Purpose: To search for novel disease-specific markers in gastric juice by investigating the protein concentrations and components in gastric juice from patients with various gastroduodenal diseases. Experimental Design: Protein concentrations and pH values in fasting gastric juice were examined in 120 healthy subjects and 39 gastric ulcer, 38 duodenal ulcer, and 31 gastric cancer patients. The protein components in gastric juice were studied by two-dimensional PAGE and mass spectrometric analysis. Results: Protein concentrations in gastric juice of patients with gastric ulcers and gastric cancer were significantly higher than those in healthy subjects (1.06 and 2.61 mg/mL versus 0.48 mg/mL; P = 0.001 and P < 0.001, respectively), and duodenal ulcer patients had lower gastric juice protein concentrations compared with healthy subjects (0.26 versus 0.48 mg/mL; P < 0.05). Gastric hypoacidity and advanced age were independent factors affecting the protein concentrations in gastric juice with odds ratios of 32.9 (95% confidence interval, 11.8-90.9) and 3.2 (95% confidence interval, 1.3-8.3), respectively. Each electrophoresis images of gastric juice could be classified into one of three patterns: basic band, specific band, or nonspecific band. The frequencies of specific band pattern in healthy subjects, gastric ulcer, duodenal ulcer, and gastric cancer patients were 6%, 42%, 6%, and 93%, respectively. Proteomic analysis revealed that α1-antitrypsin precursor was the principal peptide in the specific band. Conclusions: α1-antitrypsin precursor in gastric juice is a novel biomarker for gastric cancer and ulcer. A noninvasive method to obtain gastric juice followed by proteomic analysis may serve as a new tool to screen for gastric malignancies.

Original languageEnglish
Pages (from-to)876-883
Number of pages8
JournalClinical Cancer Research
Volume13
Issue number3
DOIs
Publication statusPublished - 2007 Feb 1

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Gastric Juice
Stomach Ulcer
Stomach Neoplasms
Biomarkers
Healthy Volunteers
Duodenal Ulcer
Proteins
Proteomics
Stomach
Duodenal Neoplasms
Confidence Intervals
Electrophoresis
Fasting
Research Design
Odds Ratio
Peptides

All Science Journal Classification (ASJC) codes

  • Oncology
  • Cancer Research

Cite this

Hsu, Ping I. ; Chen, Chung Hsuan ; Hsieh, Chieu Shia ; Chang, Wei Chao ; Lai, Kwok Hung ; Lo, Gin Ho ; Hsu, Ping Ning ; Tsay, Feng Woei ; Chen, Yu Shan ; Hsiao, Michael ; Chen, Hui Chun ; Lu, Pei-Jung. / α1-antitrypsin precursor in gastric juice is a novel biomarker for gastric cancer and ulcer. In: Clinical Cancer Research. 2007 ; Vol. 13, No. 3. pp. 876-883.
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title = "α1-antitrypsin precursor in gastric juice is a novel biomarker for gastric cancer and ulcer",
abstract = "Purpose: To search for novel disease-specific markers in gastric juice by investigating the protein concentrations and components in gastric juice from patients with various gastroduodenal diseases. Experimental Design: Protein concentrations and pH values in fasting gastric juice were examined in 120 healthy subjects and 39 gastric ulcer, 38 duodenal ulcer, and 31 gastric cancer patients. The protein components in gastric juice were studied by two-dimensional PAGE and mass spectrometric analysis. Results: Protein concentrations in gastric juice of patients with gastric ulcers and gastric cancer were significantly higher than those in healthy subjects (1.06 and 2.61 mg/mL versus 0.48 mg/mL; P = 0.001 and P < 0.001, respectively), and duodenal ulcer patients had lower gastric juice protein concentrations compared with healthy subjects (0.26 versus 0.48 mg/mL; P < 0.05). Gastric hypoacidity and advanced age were independent factors affecting the protein concentrations in gastric juice with odds ratios of 32.9 (95{\%} confidence interval, 11.8-90.9) and 3.2 (95{\%} confidence interval, 1.3-8.3), respectively. Each electrophoresis images of gastric juice could be classified into one of three patterns: basic band, specific band, or nonspecific band. The frequencies of specific band pattern in healthy subjects, gastric ulcer, duodenal ulcer, and gastric cancer patients were 6{\%}, 42{\%}, 6{\%}, and 93{\%}, respectively. Proteomic analysis revealed that α1-antitrypsin precursor was the principal peptide in the specific band. Conclusions: α1-antitrypsin precursor in gastric juice is a novel biomarker for gastric cancer and ulcer. A noninvasive method to obtain gastric juice followed by proteomic analysis may serve as a new tool to screen for gastric malignancies.",
author = "Hsu, {Ping I.} and Chen, {Chung Hsuan} and Hsieh, {Chieu Shia} and Chang, {Wei Chao} and Lai, {Kwok Hung} and Lo, {Gin Ho} and Hsu, {Ping Ning} and Tsay, {Feng Woei} and Chen, {Yu Shan} and Michael Hsiao and Chen, {Hui Chun} and Pei-Jung Lu",
year = "2007",
month = "2",
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doi = "10.1158/1078-0432.CCR-06-1404",
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Hsu, PI, Chen, CH, Hsieh, CS, Chang, WC, Lai, KH, Lo, GH, Hsu, PN, Tsay, FW, Chen, YS, Hsiao, M, Chen, HC & Lu, P-J 2007, 'α1-antitrypsin precursor in gastric juice is a novel biomarker for gastric cancer and ulcer', Clinical Cancer Research, vol. 13, no. 3, pp. 876-883. https://doi.org/10.1158/1078-0432.CCR-06-1404

α1-antitrypsin precursor in gastric juice is a novel biomarker for gastric cancer and ulcer. / Hsu, Ping I.; Chen, Chung Hsuan; Hsieh, Chieu Shia; Chang, Wei Chao; Lai, Kwok Hung; Lo, Gin Ho; Hsu, Ping Ning; Tsay, Feng Woei; Chen, Yu Shan; Hsiao, Michael; Chen, Hui Chun; Lu, Pei-Jung.

In: Clinical Cancer Research, Vol. 13, No. 3, 01.02.2007, p. 876-883.

Research output: Contribution to journalArticle

TY - JOUR

T1 - α1-antitrypsin precursor in gastric juice is a novel biomarker for gastric cancer and ulcer

AU - Hsu, Ping I.

AU - Chen, Chung Hsuan

AU - Hsieh, Chieu Shia

AU - Chang, Wei Chao

AU - Lai, Kwok Hung

AU - Lo, Gin Ho

AU - Hsu, Ping Ning

AU - Tsay, Feng Woei

AU - Chen, Yu Shan

AU - Hsiao, Michael

AU - Chen, Hui Chun

AU - Lu, Pei-Jung

PY - 2007/2/1

Y1 - 2007/2/1

N2 - Purpose: To search for novel disease-specific markers in gastric juice by investigating the protein concentrations and components in gastric juice from patients with various gastroduodenal diseases. Experimental Design: Protein concentrations and pH values in fasting gastric juice were examined in 120 healthy subjects and 39 gastric ulcer, 38 duodenal ulcer, and 31 gastric cancer patients. The protein components in gastric juice were studied by two-dimensional PAGE and mass spectrometric analysis. Results: Protein concentrations in gastric juice of patients with gastric ulcers and gastric cancer were significantly higher than those in healthy subjects (1.06 and 2.61 mg/mL versus 0.48 mg/mL; P = 0.001 and P < 0.001, respectively), and duodenal ulcer patients had lower gastric juice protein concentrations compared with healthy subjects (0.26 versus 0.48 mg/mL; P < 0.05). Gastric hypoacidity and advanced age were independent factors affecting the protein concentrations in gastric juice with odds ratios of 32.9 (95% confidence interval, 11.8-90.9) and 3.2 (95% confidence interval, 1.3-8.3), respectively. Each electrophoresis images of gastric juice could be classified into one of three patterns: basic band, specific band, or nonspecific band. The frequencies of specific band pattern in healthy subjects, gastric ulcer, duodenal ulcer, and gastric cancer patients were 6%, 42%, 6%, and 93%, respectively. Proteomic analysis revealed that α1-antitrypsin precursor was the principal peptide in the specific band. Conclusions: α1-antitrypsin precursor in gastric juice is a novel biomarker for gastric cancer and ulcer. A noninvasive method to obtain gastric juice followed by proteomic analysis may serve as a new tool to screen for gastric malignancies.

AB - Purpose: To search for novel disease-specific markers in gastric juice by investigating the protein concentrations and components in gastric juice from patients with various gastroduodenal diseases. Experimental Design: Protein concentrations and pH values in fasting gastric juice were examined in 120 healthy subjects and 39 gastric ulcer, 38 duodenal ulcer, and 31 gastric cancer patients. The protein components in gastric juice were studied by two-dimensional PAGE and mass spectrometric analysis. Results: Protein concentrations in gastric juice of patients with gastric ulcers and gastric cancer were significantly higher than those in healthy subjects (1.06 and 2.61 mg/mL versus 0.48 mg/mL; P = 0.001 and P < 0.001, respectively), and duodenal ulcer patients had lower gastric juice protein concentrations compared with healthy subjects (0.26 versus 0.48 mg/mL; P < 0.05). Gastric hypoacidity and advanced age were independent factors affecting the protein concentrations in gastric juice with odds ratios of 32.9 (95% confidence interval, 11.8-90.9) and 3.2 (95% confidence interval, 1.3-8.3), respectively. Each electrophoresis images of gastric juice could be classified into one of three patterns: basic band, specific band, or nonspecific band. The frequencies of specific band pattern in healthy subjects, gastric ulcer, duodenal ulcer, and gastric cancer patients were 6%, 42%, 6%, and 93%, respectively. Proteomic analysis revealed that α1-antitrypsin precursor was the principal peptide in the specific band. Conclusions: α1-antitrypsin precursor in gastric juice is a novel biomarker for gastric cancer and ulcer. A noninvasive method to obtain gastric juice followed by proteomic analysis may serve as a new tool to screen for gastric malignancies.

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DO - 10.1158/1078-0432.CCR-06-1404

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