3-D magnetic tweezers for investigation of mechanical properties of single DNA molecules

Chi Han Chiou; Yu Yen Huang; Meng Han Chiang; Huei Huang Lee; Gwo Bin Lee

3-D magnetic tweezers for investigation of mechanical properties of single DNA molecules

Chi Han Chiou, Yu Yen Huang, Meng Han Chiang, Huei Huang Lee, Gwo Bin Lee

Department of Engineering Science

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

1 Citation (Scopus)

Abstract

This study reports a new three-dimensional (3-D) micromachined magnetic tweezers, fabricated using MEMS (micro-electro-mechanical systems) technology, for manipulating a single 2-nm diameter DNA molecule. The new apparatus uses magnetic forces to exert over 20-pN,with less heating compared to other methods, allowing the extension of the DNA molecule over its whole contour length to investigate its entropic and elastic regions. The important elastic modulus of DNA has been explored to be 453 pN at a low ionic strength. The force-extension curve for DNA molecules is found to be consistent with theoretical models. In addition to a single DNA stretching, this study also successfully demonstrated the stretching of two parallel DNA molecules, which was never reported previously in the literature.

Original language	English
Title of host publication	19th IEEE International Conference on Micro Electro Mechanical Systems
Pages	142-145
Number of pages	4
Publication status	Published - 2006
Event	19th IEEE International Conference on Micro Electro Mechanical Systems - Istanbul, Turkey Duration: 2006 Jan 22 → 2006 Jan 26

Publication series

Name	Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)
Volume	2006
ISSN (Print)	1084-6999

Other

Other	19th IEEE International Conference on Micro Electro Mechanical Systems
Country	Turkey
City	Istanbul
Period	06-01-22 → 06-01-26

All Science Journal Classification (ASJC) codes

Electronic, Optical and Magnetic Materials
Condensed Matter Physics
Mechanical Engineering
Electrical and Electronic Engineering

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Cite this

Chiou, C. H., Huang, Y. Y., Chiang, M. H., Lee, H. H., & Lee, G. B. (2006). 3-D magnetic tweezers for investigation of mechanical properties of single DNA molecules. In 19th IEEE International Conference on Micro Electro Mechanical Systems (pp. 142-145). [1627756] (Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS); Vol. 2006).

@inproceedings{5f928a3755c143f0834c5fd45f212c10,

title = "3-D magnetic tweezers for investigation of mechanical properties of single DNA molecules",

abstract = "This study reports a new three-dimensional (3-D) micromachined magnetic tweezers, fabricated using MEMS (micro-electro-mechanical systems) technology, for manipulating a single 2-nm diameter DNA molecule. The new apparatus uses magnetic forces to exert over 20-pN,with less heating compared to other methods, allowing the extension of the DNA molecule over its whole contour length to investigate its entropic and elastic regions. The important elastic modulus of DNA has been explored to be 453 pN at a low ionic strength. The force-extension curve for DNA molecules is found to be consistent with theoretical models. In addition to a single DNA stretching, this study also successfully demonstrated the stretching of two parallel DNA molecules, which was never reported previously in the literature.",

author = "Chiou, {Chi Han} and Huang, {Yu Yen} and Chiang, {Meng Han} and Lee, {Huei Huang} and Lee, {Gwo Bin}",

year = "2006",

language = "English",

isbn = "0780394755",

series = "Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)",

pages = "142--145",

booktitle = "19th IEEE International Conference on Micro Electro Mechanical Systems",

note = "19th IEEE International Conference on Micro Electro Mechanical Systems ; Conference date: 22-01-2006 Through 26-01-2006",

}

Chiou, CH, Huang, YY, Chiang, MH, Lee, HH & Lee, GB 2006, 3-D magnetic tweezers for investigation of mechanical properties of single DNA molecules. in 19th IEEE International Conference on Micro Electro Mechanical Systems., 1627756, Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS), vol. 2006, pp. 142-145, 19th IEEE International Conference on Micro Electro Mechanical Systems, Istanbul, Turkey, 06-01-22.

3-D magnetic tweezers for investigation of mechanical properties of single DNA molecules. / Chiou, Chi Han; Huang, Yu Yen; Chiang, Meng Han; Lee, Huei Huang; Lee, Gwo Bin.

19th IEEE International Conference on Micro Electro Mechanical Systems. 2006. p. 142-145 1627756 (Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS); Vol. 2006).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

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T1 - 3-D magnetic tweezers for investigation of mechanical properties of single DNA molecules

AU - Chiou, Chi Han

AU - Huang, Yu Yen

AU - Chiang, Meng Han

AU - Lee, Huei Huang

AU - Lee, Gwo Bin

PY - 2006

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N2 - This study reports a new three-dimensional (3-D) micromachined magnetic tweezers, fabricated using MEMS (micro-electro-mechanical systems) technology, for manipulating a single 2-nm diameter DNA molecule. The new apparatus uses magnetic forces to exert over 20-pN,with less heating compared to other methods, allowing the extension of the DNA molecule over its whole contour length to investigate its entropic and elastic regions. The important elastic modulus of DNA has been explored to be 453 pN at a low ionic strength. The force-extension curve for DNA molecules is found to be consistent with theoretical models. In addition to a single DNA stretching, this study also successfully demonstrated the stretching of two parallel DNA molecules, which was never reported previously in the literature.

AB - This study reports a new three-dimensional (3-D) micromachined magnetic tweezers, fabricated using MEMS (micro-electro-mechanical systems) technology, for manipulating a single 2-nm diameter DNA molecule. The new apparatus uses magnetic forces to exert over 20-pN,with less heating compared to other methods, allowing the extension of the DNA molecule over its whole contour length to investigate its entropic and elastic regions. The important elastic modulus of DNA has been explored to be 453 pN at a low ionic strength. The force-extension curve for DNA molecules is found to be consistent with theoretical models. In addition to a single DNA stretching, this study also successfully demonstrated the stretching of two parallel DNA molecules, which was never reported previously in the literature.

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M3 - Conference contribution

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SN - 9780780394759

T3 - Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)

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BT - 19th IEEE International Conference on Micro Electro Mechanical Systems

T2 - 19th IEEE International Conference on Micro Electro Mechanical Systems

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