Abstract
This study reports a new three-dimensional (3-D) micromachined magnetic tweezers, fabricated using MEMS (micro-electro-mechanical systems) technology, for manipulating a single 2-nm diameter DNA molecule. The new apparatus uses magnetic forces to exert over 20-pN,with less heating compared to other methods, allowing the extension of the DNA molecule over its whole contour length to investigate its entropic and elastic regions. The important elastic modulus of DNA has been explored to be 453 pN at a low ionic strength. The force-extension curve for DNA molecules is found to be consistent with theoretical models. In addition to a single DNA stretching, this study also successfully demonstrated the stretching of two parallel DNA molecules, which was never reported previously in the literature.
| Original language | English |
|---|---|
| Title of host publication | 19th IEEE International Conference on Micro Electro Mechanical Systems |
| Pages | 142-145 |
| Number of pages | 4 |
| Publication status | Published - 2006 Oct 24 |
| Event | 19th IEEE International Conference on Micro Electro Mechanical Systems - Istanbul, Turkey Duration: 2006 Jan 22 → 2006 Jan 26 |
Publication series
| Name | Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS) |
|---|---|
| Volume | 2006 |
| ISSN (Print) | 1084-6999 |
Other
| Other | 19th IEEE International Conference on Micro Electro Mechanical Systems |
|---|---|
| Country | Turkey |
| City | Istanbul |
| Period | 06-01-22 → 06-01-26 |
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All Science Journal Classification (ASJC) codes
- Electronic, Optical and Magnetic Materials
- Condensed Matter Physics
- Mechanical Engineering
- Electrical and Electronic Engineering
Cite this
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3-D magnetic tweezers for investigation of mechanical properties of single DNA molecules. / Chiou, Chi Han; Huang, Yu Yen; Chiang, Meng Han; Lee, Huei-Huang; Lee, Gwo Bin.
19th IEEE International Conference on Micro Electro Mechanical Systems. 2006. p. 142-145 1627756 (Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS); Vol. 2006).Research output: Chapter in Book/Report/Conference proceeding › Conference contribution
TY - GEN
T1 - 3-D magnetic tweezers for investigation of mechanical properties of single DNA molecules
AU - Chiou, Chi Han
AU - Huang, Yu Yen
AU - Chiang, Meng Han
AU - Lee, Huei-Huang
AU - Lee, Gwo Bin
PY - 2006/10/24
Y1 - 2006/10/24
N2 - This study reports a new three-dimensional (3-D) micromachined magnetic tweezers, fabricated using MEMS (micro-electro-mechanical systems) technology, for manipulating a single 2-nm diameter DNA molecule. The new apparatus uses magnetic forces to exert over 20-pN,with less heating compared to other methods, allowing the extension of the DNA molecule over its whole contour length to investigate its entropic and elastic regions. The important elastic modulus of DNA has been explored to be 453 pN at a low ionic strength. The force-extension curve for DNA molecules is found to be consistent with theoretical models. In addition to a single DNA stretching, this study also successfully demonstrated the stretching of two parallel DNA molecules, which was never reported previously in the literature.
AB - This study reports a new three-dimensional (3-D) micromachined magnetic tweezers, fabricated using MEMS (micro-electro-mechanical systems) technology, for manipulating a single 2-nm diameter DNA molecule. The new apparatus uses magnetic forces to exert over 20-pN,with less heating compared to other methods, allowing the extension of the DNA molecule over its whole contour length to investigate its entropic and elastic regions. The important elastic modulus of DNA has been explored to be 453 pN at a low ionic strength. The force-extension curve for DNA molecules is found to be consistent with theoretical models. In addition to a single DNA stretching, this study also successfully demonstrated the stretching of two parallel DNA molecules, which was never reported previously in the literature.
UR - http://www.scopus.com/inward/record.url?scp=33750115815&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=33750115815&partnerID=8YFLogxK
M3 - Conference contribution
AN - SCOPUS:33750115815
SN - 0780394755
SN - 9780780394759
T3 - Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)
SP - 142
EP - 145
BT - 19th IEEE International Conference on Micro Electro Mechanical Systems
ER -