3-D magnetic tweezers for investigation of mechanical properties of single DNA molecules

Chi Han Chiou, Yu Yen Huang, Meng Han Chiang, Huei Huang Lee, Gwo Bin Lee

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Citation (Scopus)

Abstract

This study reports a new three-dimensional (3-D) micromachined magnetic tweezers, fabricated using MEMS (micro-electro-mechanical systems) technology, for manipulating a single 2-nm diameter DNA molecule. The new apparatus uses magnetic forces to exert over 20-pN,with less heating compared to other methods, allowing the extension of the DNA molecule over its whole contour length to investigate its entropic and elastic regions. The important elastic modulus of DNA has been explored to be 453 pN at a low ionic strength. The force-extension curve for DNA molecules is found to be consistent with theoretical models. In addition to a single DNA stretching, this study also successfully demonstrated the stretching of two parallel DNA molecules, which was never reported previously in the literature.

Original languageEnglish
Title of host publication19th IEEE International Conference on Micro Electro Mechanical Systems
Pages142-145
Number of pages4
Publication statusPublished - 2006
Event19th IEEE International Conference on Micro Electro Mechanical Systems - Istanbul, Turkey
Duration: 2006 Jan 222006 Jan 26

Publication series

NameProceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)
Volume2006
ISSN (Print)1084-6999

Other

Other19th IEEE International Conference on Micro Electro Mechanical Systems
CountryTurkey
CityIstanbul
Period06-01-2206-01-26

All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Mechanical Engineering
  • Electrical and Electronic Engineering

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  • Cite this

    Chiou, C. H., Huang, Y. Y., Chiang, M. H., Lee, H. H., & Lee, G. B. (2006). 3-D magnetic tweezers for investigation of mechanical properties of single DNA molecules. In 19th IEEE International Conference on Micro Electro Mechanical Systems (pp. 142-145). [1627756] (Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS); Vol. 2006).