Zebrafish is an ideal vertebrate for studying developmental biology and genetics due to its rapid generation rate and genetic accessibility. However, once the zebrafish has fully matured, especially wild-type lines, it develops stripes that run along the body and thus lose transparency. Owing to that, conventional optical imaging techniques will have difficulty in imaging the internal anatomy and vasculature. High frequency ultrasound imaging has been used to monitor the adult zebrafish heart blood flow. However, it is still difficult to observe the dorsal blood circulation due to its resolution. Hence, the purpose of this study is to utilize ultrafast ultrasound imaging with high frequency transducer to reconstruct the 3D blood vessel mapping of adult zebrafish.