3D microstructures array single-cell-based DEP chip for studying apoptosis of U937 & A431 cells

Cheng Hsin Chuang, Yung-Chun Lee, Huei-Sheng Huang, Fei Bin Hsiao, You Ming Hsu, Kai Hsuan Wang, Ling Ding, Hui Ju Hsu

Research output: Chapter in Book/Report/Conference proceedingConference contribution

2 Citations (Scopus)

Abstract

This study presents the design, simulation, fabrication and preliminary results of a cell trap device for detecting the early stage of apoptosis, PS (phosph-atidylserine) exposure, under single-cell resolution. The structure of biochip is consisted of ITO top electrode, PDMS flow chamber, bottom electrode array and SU-8 3D microstructure array. In order to achieve single-cell resolution, we utilize the excimer laser micromachining technique to simultaneously fabricate a chesstype bottom electrode array and a bowl-type 3D microstructure array, which design not only constructs a non-uniform electric field for trapping cells in the flow chamber by dielectrophoresis (DEP), but also enhances the positioning and immobilization of trapped cells. In addition, the optimal electrode width based on the simulation results is 30 μm for a cell size in the range of 15 20 μm such as U937 (Human histiocytic lymphoma) and A431 (Human epidermoid carcinoma) cancer cells. By using this DEP chip, we can study the physiological change of apoptosis in one single cell instead of whole cell population. As the preliminary results, 25μm latex beads can be successfully trapped with singlebead resolution in a short time. Furthermore, the time- and dose-dependent manners for A431/As203 and U937/Taol are also confirmed by agarose gel electrophoresis and flow cytometry but not on-chip monitoring. We are still working on real-time and on-chip study for development of a high-throughput drug screening with single-cell level.

Original languageEnglish
Title of host publicationProceedings of 1st IEEE International Conference on Nano Micro Engineered and Molecular Systems, 1st IEEE-NEMS
Pages1207-1210
Number of pages4
DOIs
Publication statusPublished - 2006 Dec 1
Event1st IEEE International Conference on Nano Micro Engineered and Molecular Systems, 1st IEEE-NEMS - Zhuhai, China
Duration: 2006 Jan 182006 Jan 21

Publication series

NameProceedings of 1st IEEE International Conference on Nano Micro Engineered and Molecular Systems, 1st IEEE-NEMS

Other

Other1st IEEE International Conference on Nano Micro Engineered and Molecular Systems, 1st IEEE-NEMS
CountryChina
CityZhuhai
Period06-01-1806-01-21

Fingerprint

Cell death
Electrophoresis
Apoptosis
Microstructure
Electrodes
Cells
Biochips
Flow cytometry
Latex
Micromachining
Excimer lasers
Latexes
Sepharose
Screening
Gels
Electric fields
Throughput
Fabrication
Microtechnology
Monitoring

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Materials Science (miscellaneous)

Cite this

Chuang, C. H., Lee, Y-C., Huang, H-S., Hsiao, F. B., Hsu, Y. M., Wang, K. H., ... Hsu, H. J. (2006). 3D microstructures array single-cell-based DEP chip for studying apoptosis of U937 & A431 cells. In Proceedings of 1st IEEE International Conference on Nano Micro Engineered and Molecular Systems, 1st IEEE-NEMS (pp. 1207-1210). [4135163] (Proceedings of 1st IEEE International Conference on Nano Micro Engineered and Molecular Systems, 1st IEEE-NEMS). https://doi.org/10.1109/NEMS.2006.334681
Chuang, Cheng Hsin ; Lee, Yung-Chun ; Huang, Huei-Sheng ; Hsiao, Fei Bin ; Hsu, You Ming ; Wang, Kai Hsuan ; Ding, Ling ; Hsu, Hui Ju. / 3D microstructures array single-cell-based DEP chip for studying apoptosis of U937 & A431 cells. Proceedings of 1st IEEE International Conference on Nano Micro Engineered and Molecular Systems, 1st IEEE-NEMS. 2006. pp. 1207-1210 (Proceedings of 1st IEEE International Conference on Nano Micro Engineered and Molecular Systems, 1st IEEE-NEMS).
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title = "3D microstructures array single-cell-based DEP chip for studying apoptosis of U937 & A431 cells",
abstract = "This study presents the design, simulation, fabrication and preliminary results of a cell trap device for detecting the early stage of apoptosis, PS (phosph-atidylserine) exposure, under single-cell resolution. The structure of biochip is consisted of ITO top electrode, PDMS flow chamber, bottom electrode array and SU-8 3D microstructure array. In order to achieve single-cell resolution, we utilize the excimer laser micromachining technique to simultaneously fabricate a chesstype bottom electrode array and a bowl-type 3D microstructure array, which design not only constructs a non-uniform electric field for trapping cells in the flow chamber by dielectrophoresis (DEP), but also enhances the positioning and immobilization of trapped cells. In addition, the optimal electrode width based on the simulation results is 30 μm for a cell size in the range of 15 20 μm such as U937 (Human histiocytic lymphoma) and A431 (Human epidermoid carcinoma) cancer cells. By using this DEP chip, we can study the physiological change of apoptosis in one single cell instead of whole cell population. As the preliminary results, 25μm latex beads can be successfully trapped with singlebead resolution in a short time. Furthermore, the time- and dose-dependent manners for A431/As203 and U937/Taol are also confirmed by agarose gel electrophoresis and flow cytometry but not on-chip monitoring. We are still working on real-time and on-chip study for development of a high-throughput drug screening with single-cell level.",
author = "Chuang, {Cheng Hsin} and Yung-Chun Lee and Huei-Sheng Huang and Hsiao, {Fei Bin} and Hsu, {You Ming} and Wang, {Kai Hsuan} and Ling Ding and Hsu, {Hui Ju}",
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Chuang, CH, Lee, Y-C, Huang, H-S, Hsiao, FB, Hsu, YM, Wang, KH, Ding, L & Hsu, HJ 2006, 3D microstructures array single-cell-based DEP chip for studying apoptosis of U937 & A431 cells. in Proceedings of 1st IEEE International Conference on Nano Micro Engineered and Molecular Systems, 1st IEEE-NEMS., 4135163, Proceedings of 1st IEEE International Conference on Nano Micro Engineered and Molecular Systems, 1st IEEE-NEMS, pp. 1207-1210, 1st IEEE International Conference on Nano Micro Engineered and Molecular Systems, 1st IEEE-NEMS, Zhuhai, China, 06-01-18. https://doi.org/10.1109/NEMS.2006.334681

3D microstructures array single-cell-based DEP chip for studying apoptosis of U937 & A431 cells. / Chuang, Cheng Hsin; Lee, Yung-Chun; Huang, Huei-Sheng; Hsiao, Fei Bin; Hsu, You Ming; Wang, Kai Hsuan; Ding, Ling; Hsu, Hui Ju.

Proceedings of 1st IEEE International Conference on Nano Micro Engineered and Molecular Systems, 1st IEEE-NEMS. 2006. p. 1207-1210 4135163 (Proceedings of 1st IEEE International Conference on Nano Micro Engineered and Molecular Systems, 1st IEEE-NEMS).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

TY - GEN

T1 - 3D microstructures array single-cell-based DEP chip for studying apoptosis of U937 & A431 cells

AU - Chuang, Cheng Hsin

AU - Lee, Yung-Chun

AU - Huang, Huei-Sheng

AU - Hsiao, Fei Bin

AU - Hsu, You Ming

AU - Wang, Kai Hsuan

AU - Ding, Ling

AU - Hsu, Hui Ju

PY - 2006/12/1

Y1 - 2006/12/1

N2 - This study presents the design, simulation, fabrication and preliminary results of a cell trap device for detecting the early stage of apoptosis, PS (phosph-atidylserine) exposure, under single-cell resolution. The structure of biochip is consisted of ITO top electrode, PDMS flow chamber, bottom electrode array and SU-8 3D microstructure array. In order to achieve single-cell resolution, we utilize the excimer laser micromachining technique to simultaneously fabricate a chesstype bottom electrode array and a bowl-type 3D microstructure array, which design not only constructs a non-uniform electric field for trapping cells in the flow chamber by dielectrophoresis (DEP), but also enhances the positioning and immobilization of trapped cells. In addition, the optimal electrode width based on the simulation results is 30 μm for a cell size in the range of 15 20 μm such as U937 (Human histiocytic lymphoma) and A431 (Human epidermoid carcinoma) cancer cells. By using this DEP chip, we can study the physiological change of apoptosis in one single cell instead of whole cell population. As the preliminary results, 25μm latex beads can be successfully trapped with singlebead resolution in a short time. Furthermore, the time- and dose-dependent manners for A431/As203 and U937/Taol are also confirmed by agarose gel electrophoresis and flow cytometry but not on-chip monitoring. We are still working on real-time and on-chip study for development of a high-throughput drug screening with single-cell level.

AB - This study presents the design, simulation, fabrication and preliminary results of a cell trap device for detecting the early stage of apoptosis, PS (phosph-atidylserine) exposure, under single-cell resolution. The structure of biochip is consisted of ITO top electrode, PDMS flow chamber, bottom electrode array and SU-8 3D microstructure array. In order to achieve single-cell resolution, we utilize the excimer laser micromachining technique to simultaneously fabricate a chesstype bottom electrode array and a bowl-type 3D microstructure array, which design not only constructs a non-uniform electric field for trapping cells in the flow chamber by dielectrophoresis (DEP), but also enhances the positioning and immobilization of trapped cells. In addition, the optimal electrode width based on the simulation results is 30 μm for a cell size in the range of 15 20 μm such as U937 (Human histiocytic lymphoma) and A431 (Human epidermoid carcinoma) cancer cells. By using this DEP chip, we can study the physiological change of apoptosis in one single cell instead of whole cell population. As the preliminary results, 25μm latex beads can be successfully trapped with singlebead resolution in a short time. Furthermore, the time- and dose-dependent manners for A431/As203 and U937/Taol are also confirmed by agarose gel electrophoresis and flow cytometry but not on-chip monitoring. We are still working on real-time and on-chip study for development of a high-throughput drug screening with single-cell level.

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M3 - Conference contribution

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T3 - Proceedings of 1st IEEE International Conference on Nano Micro Engineered and Molecular Systems, 1st IEEE-NEMS

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Chuang CH, Lee Y-C, Huang H-S, Hsiao FB, Hsu YM, Wang KH et al. 3D microstructures array single-cell-based DEP chip for studying apoptosis of U937 & A431 cells. In Proceedings of 1st IEEE International Conference on Nano Micro Engineered and Molecular Systems, 1st IEEE-NEMS. 2006. p. 1207-1210. 4135163. (Proceedings of 1st IEEE International Conference on Nano Micro Engineered and Molecular Systems, 1st IEEE-NEMS). https://doi.org/10.1109/NEMS.2006.334681