3D photo-crosslinking pattern scaffold for cell application by two-photon laser scanning system

Yu Fu Wang, Jen I. Liang, Chien Te Lee, Ming Long Yeh, Shean Jen Chen

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

In this study, we fabricated a photo-crosslinking, biocompatible 3D Poly(ethylene) glycol (PEG) hydrogel scaffold with inner fibrin gel for cell adhesion. We tested the material cytocompatibility with 3T3 fibroblasts then processed the cell adhesion motif inside the scaffold at regular and L-shaped 3D structural pattern by two-photon laser scanning system for further cell manipulation and recording. The results show cell could survive under UV irradiation and grew in the inner fibrin gel scaffold. The desired pattern scaffold has potential to apply in cell guided migration and physiological signal recording.

Original languageEnglish
Title of host publicationNEMS 2011 - 6th IEEE International Conference on Nano/Micro Engineered and Molecular Systems
Pages491-494
Number of pages4
DOIs
Publication statusPublished - 2011 Oct 4
Event6th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2011 - Kaohsiung, Taiwan
Duration: 2011 Feb 202011 Feb 23

Publication series

NameNEMS 2011 - 6th IEEE International Conference on Nano/Micro Engineered and Molecular Systems

Other

Other6th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2011
CountryTaiwan
CityKaohsiung
Period11-02-2011-02-23

Fingerprint

crosslinking
scanning
fibrin
photons
cells
lasers
adhesion
recording
gels
fibroblasts
glycols
manipulators
ethylene
irradiation

All Science Journal Classification (ASJC) codes

  • Atomic and Molecular Physics, and Optics

Cite this

Wang, Y. F., Liang, J. I., Lee, C. T., Yeh, M. L., & Chen, S. J. (2011). 3D photo-crosslinking pattern scaffold for cell application by two-photon laser scanning system. In NEMS 2011 - 6th IEEE International Conference on Nano/Micro Engineered and Molecular Systems (pp. 491-494). [6017400] (NEMS 2011 - 6th IEEE International Conference on Nano/Micro Engineered and Molecular Systems). https://doi.org/10.1109/NEMS.2011.6017400
Wang, Yu Fu ; Liang, Jen I. ; Lee, Chien Te ; Yeh, Ming Long ; Chen, Shean Jen. / 3D photo-crosslinking pattern scaffold for cell application by two-photon laser scanning system. NEMS 2011 - 6th IEEE International Conference on Nano/Micro Engineered and Molecular Systems. 2011. pp. 491-494 (NEMS 2011 - 6th IEEE International Conference on Nano/Micro Engineered and Molecular Systems).
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title = "3D photo-crosslinking pattern scaffold for cell application by two-photon laser scanning system",
abstract = "In this study, we fabricated a photo-crosslinking, biocompatible 3D Poly(ethylene) glycol (PEG) hydrogel scaffold with inner fibrin gel for cell adhesion. We tested the material cytocompatibility with 3T3 fibroblasts then processed the cell adhesion motif inside the scaffold at regular and L-shaped 3D structural pattern by two-photon laser scanning system for further cell manipulation and recording. The results show cell could survive under UV irradiation and grew in the inner fibrin gel scaffold. The desired pattern scaffold has potential to apply in cell guided migration and physiological signal recording.",
author = "Wang, {Yu Fu} and Liang, {Jen I.} and Lee, {Chien Te} and Yeh, {Ming Long} and Chen, {Shean Jen}",
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Wang, YF, Liang, JI, Lee, CT, Yeh, ML & Chen, SJ 2011, 3D photo-crosslinking pattern scaffold for cell application by two-photon laser scanning system. in NEMS 2011 - 6th IEEE International Conference on Nano/Micro Engineered and Molecular Systems., 6017400, NEMS 2011 - 6th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, pp. 491-494, 6th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2011, Kaohsiung, Taiwan, 11-02-20. https://doi.org/10.1109/NEMS.2011.6017400

3D photo-crosslinking pattern scaffold for cell application by two-photon laser scanning system. / Wang, Yu Fu; Liang, Jen I.; Lee, Chien Te; Yeh, Ming Long; Chen, Shean Jen.

NEMS 2011 - 6th IEEE International Conference on Nano/Micro Engineered and Molecular Systems. 2011. p. 491-494 6017400 (NEMS 2011 - 6th IEEE International Conference on Nano/Micro Engineered and Molecular Systems).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

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AB - In this study, we fabricated a photo-crosslinking, biocompatible 3D Poly(ethylene) glycol (PEG) hydrogel scaffold with inner fibrin gel for cell adhesion. We tested the material cytocompatibility with 3T3 fibroblasts then processed the cell adhesion motif inside the scaffold at regular and L-shaped 3D structural pattern by two-photon laser scanning system for further cell manipulation and recording. The results show cell could survive under UV irradiation and grew in the inner fibrin gel scaffold. The desired pattern scaffold has potential to apply in cell guided migration and physiological signal recording.

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Wang YF, Liang JI, Lee CT, Yeh ML, Chen SJ. 3D photo-crosslinking pattern scaffold for cell application by two-photon laser scanning system. In NEMS 2011 - 6th IEEE International Conference on Nano/Micro Engineered and Molecular Systems. 2011. p. 491-494. 6017400. (NEMS 2011 - 6th IEEE International Conference on Nano/Micro Engineered and Molecular Systems). https://doi.org/10.1109/NEMS.2011.6017400