3D photo-crosslinking pattern scaffold for cell application by two-photon laser scanning system

Yu Fu Wang; Jen I. Liang; Chien Te Lee; Ming Long Yeh; Shean Jen Chen

doi:10.1109/NEMS.2011.6017400

3D photo-crosslinking pattern scaffold for cell application by two-photon laser scanning system

Yu Fu Wang, Jen I. Liang, Chien Te Lee, Ming Long Yeh, Shean Jen Chen

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

In this study, we fabricated a photo-crosslinking, biocompatible 3D Poly(ethylene) glycol (PEG) hydrogel scaffold with inner fibrin gel for cell adhesion. We tested the material cytocompatibility with 3T3 fibroblasts then processed the cell adhesion motif inside the scaffold at regular and L-shaped 3D structural pattern by two-photon laser scanning system for further cell manipulation and recording. The results show cell could survive under UV irradiation and grew in the inner fibrin gel scaffold. The desired pattern scaffold has potential to apply in cell guided migration and physiological signal recording.

Original language	English
Title of host publication	NEMS 2011 - 6th IEEE International Conference on Nano/Micro Engineered and Molecular Systems
Pages	491-494
Number of pages	4
DOIs	https://doi.org/10.1109/NEMS.2011.6017400
Publication status	Published - 2011 Oct 4
Event	6th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2011 - Kaohsiung, Taiwan Duration: 2011 Feb 20 → 2011 Feb 23

Publication series

Name	NEMS 2011 - 6th IEEE International Conference on Nano/Micro Engineered and Molecular Systems

Other

Other	6th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2011
Country/Territory	Taiwan
City	Kaohsiung
Period	11-02-20 → 11-02-23

All Science Journal Classification (ASJC) codes

Atomic and Molecular Physics, and Optics

Access to Document

10.1109/NEMS.2011.6017400

Cite this

Wang, Y. F., Liang, J. I., Lee, C. T., Yeh, M. L., & Chen, S. J. (2011). 3D photo-crosslinking pattern scaffold for cell application by two-photon laser scanning system. In NEMS 2011 - 6th IEEE International Conference on Nano/Micro Engineered and Molecular Systems (pp. 491-494). [6017400] (NEMS 2011 - 6th IEEE International Conference on Nano/Micro Engineered and Molecular Systems). https://doi.org/10.1109/NEMS.2011.6017400

Wang, Yu Fu ; Liang, Jen I. ; Lee, Chien Te ; Yeh, Ming Long ; Chen, Shean Jen. / 3D photo-crosslinking pattern scaffold for cell application by two-photon laser scanning system. NEMS 2011 - 6th IEEE International Conference on Nano/Micro Engineered and Molecular Systems. 2011. pp. 491-494 (NEMS 2011 - 6th IEEE International Conference on Nano/Micro Engineered and Molecular Systems).

@inproceedings{82636cd1292a4402894b0ff2ea943208,

title = "3D photo-crosslinking pattern scaffold for cell application by two-photon laser scanning system",

abstract = "In this study, we fabricated a photo-crosslinking, biocompatible 3D Poly(ethylene) glycol (PEG) hydrogel scaffold with inner fibrin gel for cell adhesion. We tested the material cytocompatibility with 3T3 fibroblasts then processed the cell adhesion motif inside the scaffold at regular and L-shaped 3D structural pattern by two-photon laser scanning system for further cell manipulation and recording. The results show cell could survive under UV irradiation and grew in the inner fibrin gel scaffold. The desired pattern scaffold has potential to apply in cell guided migration and physiological signal recording.",

author = "Wang, {Yu Fu} and Liang, {Jen I.} and Lee, {Chien Te} and Yeh, {Ming Long} and Chen, {Shean Jen}",

year = "2011",

month = oct,

day = "4",

doi = "10.1109/NEMS.2011.6017400",

language = "English",

isbn = "9781612847757",

series = "NEMS 2011 - 6th IEEE International Conference on Nano/Micro Engineered and Molecular Systems",

pages = "491--494",

booktitle = "NEMS 2011 - 6th IEEE International Conference on Nano/Micro Engineered and Molecular Systems",

note = "6th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2011 ; Conference date: 20-02-2011 Through 23-02-2011",

}

Wang, YF, Liang, JI, Lee, CT, Yeh, ML & Chen, SJ 2011, 3D photo-crosslinking pattern scaffold for cell application by two-photon laser scanning system. in NEMS 2011 - 6th IEEE International Conference on Nano/Micro Engineered and Molecular Systems., 6017400, NEMS 2011 - 6th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, pp. 491-494, 6th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2011, Kaohsiung, Taiwan, 11-02-20. https://doi.org/10.1109/NEMS.2011.6017400

3D photo-crosslinking pattern scaffold for cell application by two-photon laser scanning system. / Wang, Yu Fu; Liang, Jen I.; Lee, Chien Te; Yeh, Ming Long; Chen, Shean Jen.

NEMS 2011 - 6th IEEE International Conference on Nano/Micro Engineered and Molecular Systems. 2011. p. 491-494 6017400 (NEMS 2011 - 6th IEEE International Conference on Nano/Micro Engineered and Molecular Systems).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

TY - GEN

T1 - 3D photo-crosslinking pattern scaffold for cell application by two-photon laser scanning system

AU - Wang, Yu Fu

AU - Liang, Jen I.

AU - Lee, Chien Te

AU - Yeh, Ming Long

AU - Chen, Shean Jen

PY - 2011/10/4

Y1 - 2011/10/4

N2 - In this study, we fabricated a photo-crosslinking, biocompatible 3D Poly(ethylene) glycol (PEG) hydrogel scaffold with inner fibrin gel for cell adhesion. We tested the material cytocompatibility with 3T3 fibroblasts then processed the cell adhesion motif inside the scaffold at regular and L-shaped 3D structural pattern by two-photon laser scanning system for further cell manipulation and recording. The results show cell could survive under UV irradiation and grew in the inner fibrin gel scaffold. The desired pattern scaffold has potential to apply in cell guided migration and physiological signal recording.

AB - In this study, we fabricated a photo-crosslinking, biocompatible 3D Poly(ethylene) glycol (PEG) hydrogel scaffold with inner fibrin gel for cell adhesion. We tested the material cytocompatibility with 3T3 fibroblasts then processed the cell adhesion motif inside the scaffold at regular and L-shaped 3D structural pattern by two-photon laser scanning system for further cell manipulation and recording. The results show cell could survive under UV irradiation and grew in the inner fibrin gel scaffold. The desired pattern scaffold has potential to apply in cell guided migration and physiological signal recording.

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Wang YF, Liang JI, Lee CT, Yeh ML, Chen SJ. 3D photo-crosslinking pattern scaffold for cell application by two-photon laser scanning system. In NEMS 2011 - 6th IEEE International Conference on Nano/Micro Engineered and Molecular Systems. 2011. p. 491-494. 6017400. (NEMS 2011 - 6th IEEE International Conference on Nano/Micro Engineered and Molecular Systems). https://doi.org/10.1109/NEMS.2011.6017400

3D photo-crosslinking pattern scaffold for cell application by two-photon laser scanning system

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