4E-BP3 regulates eIF4E-mediated nuclear mRNA export and interacts with replication protein A2

Chao Chung Chen, Jenq-Chang Lee, Ming Chung Chang

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

In nucleus, eIF4E regulates the nucleus export of specific mRNA. In this study, altered 4E-BP3 (eIF4E-binding protein 3) expression resulted in profoundly affected cyclin D1 protein levels, partially due to changes in the cytoplasmic cyclin D1 mRNA levels in both U2OS and MCF7 cells, whereas altered 4E-BP1 expression did not affect eIF4E-mediated cyclin D1 mRNA export. 4E-BP3 also affected a subset of growth promoting mRNAs exported in an eIF4-dependent manner. Furthermore, 4E-BP3 interacted with dephosphorylated RPA2 (replication protein A2). The results indicated 4E-BP3 acts as an inhibitor of eIF4E-mediated mRNA export in the examined cells, and 4E-BP3 inhibition of eIF4E-mediated mRNA export is regulated by the phosphorylation state of RPA2. Crown

Original languageEnglish
Pages (from-to)2260-2266
Number of pages7
JournalFEBS Letters
Volume586
Issue number16
DOIs
Publication statusPublished - 2012 Jul 30

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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