A 2.9-Kilobase noncoding nuclear RNA functions in the establishment of persistent Hz-1 viral infection

Yu Chan Chao, Song Tay Lee, Ming Chuan Chang, Hong Hwa Chen, Shih Shun Chen, Tzong Yuan Wu, Fu Hwa Liu, Err Lieh Hsu, Roger F. Hou

Research output: Contribution to journalArticlepeer-review

30 Citations (Scopus)

Abstract

Differential viral gene expression during both productive and persistent infections of Hz-1 virus in insect cells was elucidated. Despite more than 100 viral transcripts being expressed during productive vital infection, massive vital gene shutoff was observed during viral persistency, leaving the 2.9-kb persistence-associated transcript 1 (PAT1) as the only detectable viral RNA. Persistence-associated gene 1 (pag1), which encodes PAT1, was cloned and found to contain no significant open reading frames. PAT1 is not associated with the cellular translation machinery and is located exclusively in the nucleus. Further experiments showed that PAT1 is functional in the establishment of persistent Hz-1 viral infection in the cells. All the evidence collectively indicates that PAT1 is a novel nuclear transcript of vital origin. Our results showed that although PAT1 and XIST RNA, a mammalian X-inactive specific transcript, are transcribed by different genes, they have interesting similarities.

Original languageEnglish
Pages (from-to)2233-2245
Number of pages13
JournalJournal of Virology
Volume72
Issue number3
DOIs
Publication statusPublished - 1998 Mar

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

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