A bacteria injection scheme for in situ bioaugmentation

C. S. Liu, M. C. Tom Kuo, C. Y. Su, Y. C. Chen, W. C. Cheng, C. Y. Chou, K. F. Liang, Y. L. Han, C. H. Lin

Research output: Contribution to journalArticlepeer-review


This article presents an innovative design for inoculating the desired organisms to stratified geological layers at desired rates during in-situ bioaugmentation. The new delivery system consists of intermittent porous tubes connected in series with impermeable polyethylene tubes that run horizontally in each stratified layer of a contaminated aquifer. A bioaugmentation test using the new delivery system was conducted to inject an enriched culture of Escherichia coli (E. coli). Results of the test indicated that the distribution of E. coli through each porous tube was fairly uniform. A mathematical model previously developed to calculate the distribution of water flow through each porous tube was modified to calculate the distribution of E. coli. Geological layers often have different hydraulic conductivities. By controlling the permeability and the length of porous tubes placed in stratified layers, the new design provides a means to selectively deliver aqueous bacteria to various layers at desired rates according to aquifer heterogeneity.

Original languageEnglish
Pages (from-to)1079-1085
Number of pages7
JournalJournal of Environmental Science and Health - Part A Toxic/Hazardous Substances and Environmental Engineering
Issue number9
Publication statusPublished - 2013 Jul 15

All Science Journal Classification (ASJC) codes

  • Environmental Engineering


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