37 Citations (Scopus)

Abstract

Recently, it has been found that thiolated oligonucleotides can be used to direct the assembly of gold colloids into ordered functional nanostructures. However, approaches for fabricating Au/DNA conjugates by using a linear, functional, double-stranded (ds) DNA fragment that directly binds to a Au NP surface have not been reported. In this paper, we used a ligase-dependent strategy to synthesize a 1.7 kb, thiolated dsDNA fragment and generated novel nanocrystals by coupling it to 13 nm Au NPs. The conjugates contained Au/EGFP with EGFP/ Au molar ratios of 1:1, 2:1, and 3:1, as determined by their electrophoretic mobility and AFM imaging. The Au/EGFP nanoconjugates could still be digested by restriction endonuclease (RE) and expressed as functional proteins in mammalian cells, indicating the biological activities of DNA fragments were retained after conjugation with Au NPs. This biological strategy for fabrication of Au/DNA conjugates may be applied to molecular imaging, nanomedicine, and nanobiosensor technology.

Original languageEnglish
Pages (from-to)1209-1212
Number of pages4
JournalNano letters
Volume4
Issue number7
DOIs
Publication statusPublished - 2004 Jul 1

Fingerprint

retaining
Bioactivity
DNA
deoxyribonucleic acid
Nanoparticles
Fabrication
nanoparticles
fabrication
fragments
Nanoconjugates
Medical nanotechnology
Molecular imaging
Gold Colloid
Electrophoretic mobility
oligonucleotides
Oligonucleotides
DNA Restriction Enzymes
Ligases
activity (biology)
Colloids

All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Chemistry(all)
  • Materials Science(all)
  • Condensed Matter Physics
  • Mechanical Engineering

Cite this

@article{ed66112fcdba490091274ee1ff689e71,
title = "A biological strategy for fabrication of Au/EGFP nanoparticle conjugates retaining bioactivity",
abstract = "Recently, it has been found that thiolated oligonucleotides can be used to direct the assembly of gold colloids into ordered functional nanostructures. However, approaches for fabricating Au/DNA conjugates by using a linear, functional, double-stranded (ds) DNA fragment that directly binds to a Au NP surface have not been reported. In this paper, we used a ligase-dependent strategy to synthesize a 1.7 kb, thiolated dsDNA fragment and generated novel nanocrystals by coupling it to 13 nm Au NPs. The conjugates contained Au/EGFP with EGFP/ Au molar ratios of 1:1, 2:1, and 3:1, as determined by their electrophoretic mobility and AFM imaging. The Au/EGFP nanoconjugates could still be digested by restriction endonuclease (RE) and expressed as functional proteins in mammalian cells, indicating the biological activities of DNA fragments were retained after conjugation with Au NPs. This biological strategy for fabrication of Au/DNA conjugates may be applied to molecular imaging, nanomedicine, and nanobiosensor technology.",
author = "Tsai, {Chiau Yuang} and Ai-Li Shiau and Cheng, {Pai Chiao} and Dar-Bin Shieh and Dong-Hwang Chen and Chen-Hsi Chou and Chen-Sheng Yeh and Chao-Liang Wu",
year = "2004",
month = "7",
day = "1",
doi = "10.1021/nl049523l",
language = "English",
volume = "4",
pages = "1209--1212",
journal = "Nano Letters",
issn = "1530-6984",
publisher = "American Chemical Society",
number = "7",

}

A biological strategy for fabrication of Au/EGFP nanoparticle conjugates retaining bioactivity. / Tsai, Chiau Yuang; Shiau, Ai-Li; Cheng, Pai Chiao; Shieh, Dar-Bin; Chen, Dong-Hwang; Chou, Chen-Hsi; Yeh, Chen-Sheng; Wu, Chao-Liang.

In: Nano letters, Vol. 4, No. 7, 01.07.2004, p. 1209-1212.

Research output: Contribution to journalArticle

TY - JOUR

T1 - A biological strategy for fabrication of Au/EGFP nanoparticle conjugates retaining bioactivity

AU - Tsai, Chiau Yuang

AU - Shiau, Ai-Li

AU - Cheng, Pai Chiao

AU - Shieh, Dar-Bin

AU - Chen, Dong-Hwang

AU - Chou, Chen-Hsi

AU - Yeh, Chen-Sheng

AU - Wu, Chao-Liang

PY - 2004/7/1

Y1 - 2004/7/1

N2 - Recently, it has been found that thiolated oligonucleotides can be used to direct the assembly of gold colloids into ordered functional nanostructures. However, approaches for fabricating Au/DNA conjugates by using a linear, functional, double-stranded (ds) DNA fragment that directly binds to a Au NP surface have not been reported. In this paper, we used a ligase-dependent strategy to synthesize a 1.7 kb, thiolated dsDNA fragment and generated novel nanocrystals by coupling it to 13 nm Au NPs. The conjugates contained Au/EGFP with EGFP/ Au molar ratios of 1:1, 2:1, and 3:1, as determined by their electrophoretic mobility and AFM imaging. The Au/EGFP nanoconjugates could still be digested by restriction endonuclease (RE) and expressed as functional proteins in mammalian cells, indicating the biological activities of DNA fragments were retained after conjugation with Au NPs. This biological strategy for fabrication of Au/DNA conjugates may be applied to molecular imaging, nanomedicine, and nanobiosensor technology.

AB - Recently, it has been found that thiolated oligonucleotides can be used to direct the assembly of gold colloids into ordered functional nanostructures. However, approaches for fabricating Au/DNA conjugates by using a linear, functional, double-stranded (ds) DNA fragment that directly binds to a Au NP surface have not been reported. In this paper, we used a ligase-dependent strategy to synthesize a 1.7 kb, thiolated dsDNA fragment and generated novel nanocrystals by coupling it to 13 nm Au NPs. The conjugates contained Au/EGFP with EGFP/ Au molar ratios of 1:1, 2:1, and 3:1, as determined by their electrophoretic mobility and AFM imaging. The Au/EGFP nanoconjugates could still be digested by restriction endonuclease (RE) and expressed as functional proteins in mammalian cells, indicating the biological activities of DNA fragments were retained after conjugation with Au NPs. This biological strategy for fabrication of Au/DNA conjugates may be applied to molecular imaging, nanomedicine, and nanobiosensor technology.

UR - http://www.scopus.com/inward/record.url?scp=4143091322&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=4143091322&partnerID=8YFLogxK

U2 - 10.1021/nl049523l

DO - 10.1021/nl049523l

M3 - Article

AN - SCOPUS:4143091322

VL - 4

SP - 1209

EP - 1212

JO - Nano Letters

JF - Nano Letters

SN - 1530-6984

IS - 7

ER -