A. castellanii and P. aeruginosa mutually exacerbate damage to corneal cells during coinfection

Chun Hsien Chen, Chen-Chieh Liao, Yu Jen Wang, Fu Chin Huang, Wei Chen Lin

Research output: Contribution to journalArticlepeer-review


Microorganisms that are involved in microbial invasion keratitis infiltrate the cornea and cause eye pain. Despite the administration of antimicrobial treatment, patients with amoebic keratitis develop bacterial coinfections. Pseudomonas aeruginosa infection accounts for 50% of the reported bacterial coinfections in these patients. However, the role of pathogenic bacteria in amoeba-induced corneal damage remains unclear. In our current study, we conducted assays to examine cytopathic effects and observed increased corneal cell damage in the group with secondary P. aeruginosa infections. Imaging revealed that the presence of A. castellanii enhanced the accumulation of P. aeruginosa in areas of cell monolayer leakage. We demonstrated that treatment of P. aeruginosa with amoeba-soluble antigen resulted in higher adhesion ability, increased biofilm formation, and more severe corneal cell damage. Overall, this research significantly contributes to our understanding of the risk of P. aeruginosa coinfection in the progression of Acanthamoeba keratitis.

Original languageEnglish
JournalMicrobiology spectrum
Issue number1
Publication statusPublished - 2024 Jan

All Science Journal Classification (ASJC) codes

  • Physiology
  • Ecology
  • General Immunology and Microbiology
  • Genetics
  • Microbiology (medical)
  • Cell Biology
  • Infectious Diseases


Dive into the research topics of 'A. castellanii and P. aeruginosa mutually exacerbate damage to corneal cells during coinfection'. Together they form a unique fingerprint.

Cite this