A cell wall protein (YqgA) is genetically related to the cell wall-degrading DL-endopeptidases in Bacillus subtilis

Masayuki Hashimoto, Kentaro Fujikura, Yukiko Miyake, Yuhei Higashitsuji, Yuuka Kiriyama, Tatsuhito Tanaka, Hiroki Yamamoto, Junichi Sekiguchi

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The Gram-positive bacterium Bacillus subtilis has a thick cell wall. The cell wall contains various proteins, both for secretion and for peptidoglycan (PG) maintenance. Penicillin-binding proteins for PG synthesis, PG hydrolases (autolysins), and regulator proteins for the autolysins are the known components of the PG maintenance system. YqgA was identified as an abundant protein attached to the cell wall of B. subtilis through a proteomics analysis. The YqgA protein was localized at cell division sites during the transition period between the exponential and the stationary phases. YqgA localization was affected by mutations in the DL-endopeptidases (DLEPases), which are the autolysins involved in cell morphogenesis. Furthermore, yqgA mutations on a background of defective DLEPases led to delays in cell growth and cell morphological changes. These results demonstrate that yqgA is genetically related to the genes encoding DLEPases involved in cell morphogenesis.

Original languageEnglish
Pages (from-to)1428-1434
Number of pages7
JournalBioscience, Biotechnology and Biochemistry
Volume78
Issue number8
DOIs
Publication statusPublished - 2014 Jan 1

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N-Acetylmuramoyl-L-alanine Amidase
Endopeptidases
Bacilli
Bacillus subtilis
Cell Wall
Peptidoglycan
Cells
Morphogenesis
Proteins
Maintenance
Penicillin-Binding Proteins
Mutation
Gene encoding
Cell growth
Gram-Positive Bacteria
Cell Division
Proteomics
Bacteria
Growth
Genes

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

Cite this

Hashimoto, Masayuki ; Fujikura, Kentaro ; Miyake, Yukiko ; Higashitsuji, Yuhei ; Kiriyama, Yuuka ; Tanaka, Tatsuhito ; Yamamoto, Hiroki ; Sekiguchi, Junichi. / A cell wall protein (YqgA) is genetically related to the cell wall-degrading DL-endopeptidases in Bacillus subtilis. In: Bioscience, Biotechnology and Biochemistry. 2014 ; Vol. 78, No. 8. pp. 1428-1434.
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A cell wall protein (YqgA) is genetically related to the cell wall-degrading DL-endopeptidases in Bacillus subtilis. / Hashimoto, Masayuki; Fujikura, Kentaro; Miyake, Yukiko; Higashitsuji, Yuhei; Kiriyama, Yuuka; Tanaka, Tatsuhito; Yamamoto, Hiroki; Sekiguchi, Junichi.

In: Bioscience, Biotechnology and Biochemistry, Vol. 78, No. 8, 01.01.2014, p. 1428-1434.

Research output: Contribution to journalArticle

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