A comparison of the expression of immunity-related rag 1 and ikaros genes with histogenesis of the thymus in Epinephelus malabaricus (Bloch & Schneider)

John Han You Lin, Han Tso Lin, Carmen Lopez, Tzong-Yueh Chen, Ming Shyan Chen, Huey Lang Yang

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Expression of the immunity-related ikaros and rag1 genes during development signals the onset of lymphopoiesis in vertebrates. Partial sequences of ikaros and rag1 in Epinephelus malabaricus were cloned by degenerate primer-mediated reverse transcriptase polymerase chain reaction (RT-PCR). Their expression profiles in the thymus, head kidney, trunk kidney, spleen, intestine, brain, liver and pancreas, and the onset of expression at various developmental stages, were examined by RT-PCR using identical primers. Expressions of both ikaros and rag1 genes were detected as early as 3 days post fertilization (dpf). The thymus is thought to be the first organ of lymphopoiesis to develop in fish; its histogenesis in E. malabaricus was examined and used for comparison. To avoid the potential influence of environmental factors on different hatches, both age and larval morphology were used as indicators of maturation to define the stage of development. A bud-like thymus was observed at 9 dpf; lymphopoietic cells appeared at 19 dpf; and further development, such as cortex/medulla differentiation and the appearance of lymphocytes, occurred by 26 dpf. Trabecula development was detected at 41 dpf. Based on the histological evidence, the lymphoid cell expressed rag1 before the thymus developed, suggesting that extra-thymic lymphopoiesis takes place in E. malabaricus, and that a functional immune system might develop early in the metamorphosis stage.

Original languageEnglish
Pages (from-to)252-262
Number of pages11
JournalAquaculture Research
Volume39
Issue number3
DOIs
Publication statusPublished - 2008 Feb 1

Fingerprint

Epinephelus malabaricus
immunity
polymerase chain reaction
morphogenesis
gene
immune system
metamorphosis
reverse transcriptase polymerase chain reaction
developmental stage
bud
kidneys
brain
maturation
vertebrate
environmental factor
genes
pancreas
tree trunk
cortex
spleen

All Science Journal Classification (ASJC) codes

  • Aquatic Science

Cite this

Lin, John Han You ; Lin, Han Tso ; Lopez, Carmen ; Chen, Tzong-Yueh ; Chen, Ming Shyan ; Yang, Huey Lang. / A comparison of the expression of immunity-related rag 1 and ikaros genes with histogenesis of the thymus in Epinephelus malabaricus (Bloch & Schneider). In: Aquaculture Research. 2008 ; Vol. 39, No. 3. pp. 252-262.
@article{ecf684b48a7a4ed1b98f1e1b41469cd4,
title = "A comparison of the expression of immunity-related rag 1 and ikaros genes with histogenesis of the thymus in Epinephelus malabaricus (Bloch & Schneider)",
abstract = "Expression of the immunity-related ikaros and rag1 genes during development signals the onset of lymphopoiesis in vertebrates. Partial sequences of ikaros and rag1 in Epinephelus malabaricus were cloned by degenerate primer-mediated reverse transcriptase polymerase chain reaction (RT-PCR). Their expression profiles in the thymus, head kidney, trunk kidney, spleen, intestine, brain, liver and pancreas, and the onset of expression at various developmental stages, were examined by RT-PCR using identical primers. Expressions of both ikaros and rag1 genes were detected as early as 3 days post fertilization (dpf). The thymus is thought to be the first organ of lymphopoiesis to develop in fish; its histogenesis in E. malabaricus was examined and used for comparison. To avoid the potential influence of environmental factors on different hatches, both age and larval morphology were used as indicators of maturation to define the stage of development. A bud-like thymus was observed at 9 dpf; lymphopoietic cells appeared at 19 dpf; and further development, such as cortex/medulla differentiation and the appearance of lymphocytes, occurred by 26 dpf. Trabecula development was detected at 41 dpf. Based on the histological evidence, the lymphoid cell expressed rag1 before the thymus developed, suggesting that extra-thymic lymphopoiesis takes place in E. malabaricus, and that a functional immune system might develop early in the metamorphosis stage.",
author = "Lin, {John Han You} and Lin, {Han Tso} and Carmen Lopez and Tzong-Yueh Chen and Chen, {Ming Shyan} and Yang, {Huey Lang}",
year = "2008",
month = "2",
day = "1",
doi = "10.1111/j.1365-2109.2007.01871.x",
language = "English",
volume = "39",
pages = "252--262",
journal = "Aquaculture Research",
issn = "1355-557X",
publisher = "Wiley-Blackwell",
number = "3",

}

A comparison of the expression of immunity-related rag 1 and ikaros genes with histogenesis of the thymus in Epinephelus malabaricus (Bloch & Schneider). / Lin, John Han You; Lin, Han Tso; Lopez, Carmen; Chen, Tzong-Yueh; Chen, Ming Shyan; Yang, Huey Lang.

In: Aquaculture Research, Vol. 39, No. 3, 01.02.2008, p. 252-262.

Research output: Contribution to journalArticle

TY - JOUR

T1 - A comparison of the expression of immunity-related rag 1 and ikaros genes with histogenesis of the thymus in Epinephelus malabaricus (Bloch & Schneider)

AU - Lin, John Han You

AU - Lin, Han Tso

AU - Lopez, Carmen

AU - Chen, Tzong-Yueh

AU - Chen, Ming Shyan

AU - Yang, Huey Lang

PY - 2008/2/1

Y1 - 2008/2/1

N2 - Expression of the immunity-related ikaros and rag1 genes during development signals the onset of lymphopoiesis in vertebrates. Partial sequences of ikaros and rag1 in Epinephelus malabaricus were cloned by degenerate primer-mediated reverse transcriptase polymerase chain reaction (RT-PCR). Their expression profiles in the thymus, head kidney, trunk kidney, spleen, intestine, brain, liver and pancreas, and the onset of expression at various developmental stages, were examined by RT-PCR using identical primers. Expressions of both ikaros and rag1 genes were detected as early as 3 days post fertilization (dpf). The thymus is thought to be the first organ of lymphopoiesis to develop in fish; its histogenesis in E. malabaricus was examined and used for comparison. To avoid the potential influence of environmental factors on different hatches, both age and larval morphology were used as indicators of maturation to define the stage of development. A bud-like thymus was observed at 9 dpf; lymphopoietic cells appeared at 19 dpf; and further development, such as cortex/medulla differentiation and the appearance of lymphocytes, occurred by 26 dpf. Trabecula development was detected at 41 dpf. Based on the histological evidence, the lymphoid cell expressed rag1 before the thymus developed, suggesting that extra-thymic lymphopoiesis takes place in E. malabaricus, and that a functional immune system might develop early in the metamorphosis stage.

AB - Expression of the immunity-related ikaros and rag1 genes during development signals the onset of lymphopoiesis in vertebrates. Partial sequences of ikaros and rag1 in Epinephelus malabaricus were cloned by degenerate primer-mediated reverse transcriptase polymerase chain reaction (RT-PCR). Their expression profiles in the thymus, head kidney, trunk kidney, spleen, intestine, brain, liver and pancreas, and the onset of expression at various developmental stages, were examined by RT-PCR using identical primers. Expressions of both ikaros and rag1 genes were detected as early as 3 days post fertilization (dpf). The thymus is thought to be the first organ of lymphopoiesis to develop in fish; its histogenesis in E. malabaricus was examined and used for comparison. To avoid the potential influence of environmental factors on different hatches, both age and larval morphology were used as indicators of maturation to define the stage of development. A bud-like thymus was observed at 9 dpf; lymphopoietic cells appeared at 19 dpf; and further development, such as cortex/medulla differentiation and the appearance of lymphocytes, occurred by 26 dpf. Trabecula development was detected at 41 dpf. Based on the histological evidence, the lymphoid cell expressed rag1 before the thymus developed, suggesting that extra-thymic lymphopoiesis takes place in E. malabaricus, and that a functional immune system might develop early in the metamorphosis stage.

UR - http://www.scopus.com/inward/record.url?scp=38549132257&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=38549132257&partnerID=8YFLogxK

U2 - 10.1111/j.1365-2109.2007.01871.x

DO - 10.1111/j.1365-2109.2007.01871.x

M3 - Article

VL - 39

SP - 252

EP - 262

JO - Aquaculture Research

JF - Aquaculture Research

SN - 1355-557X

IS - 3

ER -