A convenient method to extract matrix-assisted laser desorption/ionization mass spectrometry spectra from phosphate-containing peptide mixtures

Sheng Yu Huang, Jue Liang Hsu, Nicholas A. Morrice, Chin Jen Wu, Shu-Hui Chen

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Here we report that the addition of HCl or HNO3 to the matrix at a limited concentration dramatically increases the signal-to-noise ratio of the matrix-assisted laser desorption/ionization mass spectrometry spectrum of phosphate-containing peptide mixtures such as those obtained from an immobilised metal affinity capture eluent or a phosphate-containing tryptic digest. These improved spectra permitted both peptide identification and the determination of protein phosphorylation sites. In comparison to existing methods for removing salts, this method requires less sample manipulation and thus less sample loss is expected.

Original languageEnglish
Pages (from-to)1935-1938
Number of pages4
JournalProteomics
Volume4
Issue number7
DOIs
Publication statusPublished - 2004 Jul 1

Fingerprint

Matrix-Assisted Laser Desorption-Ionization Mass Spectrometry
Ionization
Mass spectrometry
Desorption
Phosphates
Peptides
Phosphorylation
Lasers
Signal-To-Noise Ratio
Signal to noise ratio
Salts
Metals
Proteins

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology

Cite this

Huang, Sheng Yu ; Hsu, Jue Liang ; Morrice, Nicholas A. ; Wu, Chin Jen ; Chen, Shu-Hui. / A convenient method to extract matrix-assisted laser desorption/ionization mass spectrometry spectra from phosphate-containing peptide mixtures. In: Proteomics. 2004 ; Vol. 4, No. 7. pp. 1935-1938.
@article{1e1bdccb80a44e7481f00a55fa4d7075,
title = "A convenient method to extract matrix-assisted laser desorption/ionization mass spectrometry spectra from phosphate-containing peptide mixtures",
abstract = "Here we report that the addition of HCl or HNO3 to the matrix at a limited concentration dramatically increases the signal-to-noise ratio of the matrix-assisted laser desorption/ionization mass spectrometry spectrum of phosphate-containing peptide mixtures such as those obtained from an immobilised metal affinity capture eluent or a phosphate-containing tryptic digest. These improved spectra permitted both peptide identification and the determination of protein phosphorylation sites. In comparison to existing methods for removing salts, this method requires less sample manipulation and thus less sample loss is expected.",
author = "Huang, {Sheng Yu} and Hsu, {Jue Liang} and Morrice, {Nicholas A.} and Wu, {Chin Jen} and Shu-Hui Chen",
year = "2004",
month = "7",
day = "1",
doi = "10.1002/pmic.200300717",
language = "English",
volume = "4",
pages = "1935--1938",
journal = "Proteomics",
issn = "1615-9853",
publisher = "Wiley-VCH Verlag",
number = "7",

}

A convenient method to extract matrix-assisted laser desorption/ionization mass spectrometry spectra from phosphate-containing peptide mixtures. / Huang, Sheng Yu; Hsu, Jue Liang; Morrice, Nicholas A.; Wu, Chin Jen; Chen, Shu-Hui.

In: Proteomics, Vol. 4, No. 7, 01.07.2004, p. 1935-1938.

Research output: Contribution to journalArticle

TY - JOUR

T1 - A convenient method to extract matrix-assisted laser desorption/ionization mass spectrometry spectra from phosphate-containing peptide mixtures

AU - Huang, Sheng Yu

AU - Hsu, Jue Liang

AU - Morrice, Nicholas A.

AU - Wu, Chin Jen

AU - Chen, Shu-Hui

PY - 2004/7/1

Y1 - 2004/7/1

N2 - Here we report that the addition of HCl or HNO3 to the matrix at a limited concentration dramatically increases the signal-to-noise ratio of the matrix-assisted laser desorption/ionization mass spectrometry spectrum of phosphate-containing peptide mixtures such as those obtained from an immobilised metal affinity capture eluent or a phosphate-containing tryptic digest. These improved spectra permitted both peptide identification and the determination of protein phosphorylation sites. In comparison to existing methods for removing salts, this method requires less sample manipulation and thus less sample loss is expected.

AB - Here we report that the addition of HCl or HNO3 to the matrix at a limited concentration dramatically increases the signal-to-noise ratio of the matrix-assisted laser desorption/ionization mass spectrometry spectrum of phosphate-containing peptide mixtures such as those obtained from an immobilised metal affinity capture eluent or a phosphate-containing tryptic digest. These improved spectra permitted both peptide identification and the determination of protein phosphorylation sites. In comparison to existing methods for removing salts, this method requires less sample manipulation and thus less sample loss is expected.

UR - http://www.scopus.com/inward/record.url?scp=3042777263&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=3042777263&partnerID=8YFLogxK

U2 - 10.1002/pmic.200300717

DO - 10.1002/pmic.200300717

M3 - Article

VL - 4

SP - 1935

EP - 1938

JO - Proteomics

JF - Proteomics

SN - 1615-9853

IS - 7

ER -