A DEP chip with arc-shape microelectrode arrays for the separation of different-size particles

Cheng Hsin Chunag, Yu Chi Chen, You Ming Hsu, Huei-Sheng Huang, Fei Bin Hsiao, Chin Hung Wang

Research output: Chapter in Book/Report/Conference proceedingConference contribution

4 Citations (Scopus)

Abstract

This paper presents a microfluidic chip with arcshape electrodes array (ASEA) for separation of different-size particles/Cells based on negative dielectrophoresis (nDEP). The purpose of ASEA is to generate the dielectrophoretic force perpendicular to the microchannel direction within the gap between each pairs of arc-shape electrodes. The DEP chip consisted of two regions of ASEA; first ASEA was design for aligning all particles floating along with the wall of microchannel before enter the second ASEA region; the configuration of second ASEA was upside down with first region, thus, the DEP force could move particles from the one side of microchannel toward the other side with different magnitude corresponding to its size, consequently, different size of particles can be separated within the second ASEA region. In this study, three typically electrode shapes, trapezoidal shape, convex-arc shape and concave-arc shape, were investigated for constructing an electric field gradient along the perpendicular direction to fluid flow by numerical simulation. A convex arc-shape electrode can provide better separation effect due to the larger effective area of DEP force and relative small variation of VE2 as indicated in the simulation results. Therefore, we fabricated a PDMS microchannel with two convex ASEAs for demonstration of the separation function between 19pm and 8pm polystyrene particles. This method can provide a continuous and high throughput separation of different-size bioparticles once they have similar dielectric properties that cannot separated by traditional DEP approach.

Original languageEnglish
Title of host publication4th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2009
Pages854-857
Number of pages4
DOIs
Publication statusPublished - 2009 Oct 12
Event4th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2009 - Shenzhen, China
Duration: 2009 Jan 52009 Jan 8

Publication series

Name4th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2009

Other

Other4th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2009
CountryChina
CityShenzhen
Period09-01-0509-01-08

Fingerprint

Microelectrodes
Particle size
Electrodes
Microchannels
Electrophoresis
Microfluidics
Dielectric properties
Flow of fluids
Polystyrenes
Demonstrations
Electric fields
Throughput

All Science Journal Classification (ASJC) codes

  • Electrical and Electronic Engineering

Cite this

Chunag, C. H., Chen, Y. C., Hsu, Y. M., Huang, H-S., Hsiao, F. B., & Wang, C. H. (2009). A DEP chip with arc-shape microelectrode arrays for the separation of different-size particles. In 4th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2009 (pp. 854-857). [5068709] (4th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2009). https://doi.org/10.1109/NEMS.2009.5068709
Chunag, Cheng Hsin ; Chen, Yu Chi ; Hsu, You Ming ; Huang, Huei-Sheng ; Hsiao, Fei Bin ; Wang, Chin Hung. / A DEP chip with arc-shape microelectrode arrays for the separation of different-size particles. 4th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2009. 2009. pp. 854-857 (4th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2009).
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abstract = "This paper presents a microfluidic chip with arcshape electrodes array (ASEA) for separation of different-size particles/Cells based on negative dielectrophoresis (nDEP). The purpose of ASEA is to generate the dielectrophoretic force perpendicular to the microchannel direction within the gap between each pairs of arc-shape electrodes. The DEP chip consisted of two regions of ASEA; first ASEA was design for aligning all particles floating along with the wall of microchannel before enter the second ASEA region; the configuration of second ASEA was upside down with first region, thus, the DEP force could move particles from the one side of microchannel toward the other side with different magnitude corresponding to its size, consequently, different size of particles can be separated within the second ASEA region. In this study, three typically electrode shapes, trapezoidal shape, convex-arc shape and concave-arc shape, were investigated for constructing an electric field gradient along the perpendicular direction to fluid flow by numerical simulation. A convex arc-shape electrode can provide better separation effect due to the larger effective area of DEP force and relative small variation of VE2 as indicated in the simulation results. Therefore, we fabricated a PDMS microchannel with two convex ASEAs for demonstration of the separation function between 19pm and 8pm polystyrene particles. This method can provide a continuous and high throughput separation of different-size bioparticles once they have similar dielectric properties that cannot separated by traditional DEP approach.",
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Chunag, CH, Chen, YC, Hsu, YM, Huang, H-S, Hsiao, FB & Wang, CH 2009, A DEP chip with arc-shape microelectrode arrays for the separation of different-size particles. in 4th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2009., 5068709, 4th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2009, pp. 854-857, 4th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2009, Shenzhen, China, 09-01-05. https://doi.org/10.1109/NEMS.2009.5068709

A DEP chip with arc-shape microelectrode arrays for the separation of different-size particles. / Chunag, Cheng Hsin; Chen, Yu Chi; Hsu, You Ming; Huang, Huei-Sheng; Hsiao, Fei Bin; Wang, Chin Hung.

4th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2009. 2009. p. 854-857 5068709 (4th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2009).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

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N2 - This paper presents a microfluidic chip with arcshape electrodes array (ASEA) for separation of different-size particles/Cells based on negative dielectrophoresis (nDEP). The purpose of ASEA is to generate the dielectrophoretic force perpendicular to the microchannel direction within the gap between each pairs of arc-shape electrodes. The DEP chip consisted of two regions of ASEA; first ASEA was design for aligning all particles floating along with the wall of microchannel before enter the second ASEA region; the configuration of second ASEA was upside down with first region, thus, the DEP force could move particles from the one side of microchannel toward the other side with different magnitude corresponding to its size, consequently, different size of particles can be separated within the second ASEA region. In this study, three typically electrode shapes, trapezoidal shape, convex-arc shape and concave-arc shape, were investigated for constructing an electric field gradient along the perpendicular direction to fluid flow by numerical simulation. A convex arc-shape electrode can provide better separation effect due to the larger effective area of DEP force and relative small variation of VE2 as indicated in the simulation results. Therefore, we fabricated a PDMS microchannel with two convex ASEAs for demonstration of the separation function between 19pm and 8pm polystyrene particles. This method can provide a continuous and high throughput separation of different-size bioparticles once they have similar dielectric properties that cannot separated by traditional DEP approach.

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Chunag CH, Chen YC, Hsu YM, Huang H-S, Hsiao FB, Wang CH. A DEP chip with arc-shape microelectrode arrays for the separation of different-size particles. In 4th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2009. 2009. p. 854-857. 5068709. (4th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2009). https://doi.org/10.1109/NEMS.2009.5068709