A developed competitive immunoassay based on impedance measurements for methamphetamine detection

Chia Hsien Yeh, Wei Ting Wang, Pi Lan Shen, Yu-Cheng Lin

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

In this study, an electro-microchip was successfully used to detect the impedance signals of various methamphetamine (MET) concentrations based on the developed competitive immunoassay method. MET is a commonly used drug often abused by drug addicts and can cause irregular behavior; therefore, MET concentration detection is important for quantitative analysis. In this study, gold nanoparticles (AuNPs) were introduced into an electro-microchip through the specific binding of antibodies, thus constructing a bridge between two electrodes and allowing electrons to move between them. The decreasing impedance value can be easily measured with a commercial LCR meter. According to the collected measurements, a significant difference was observed in impedance signals after 13 min when MET concentrations were reduced. Additionally, a clear, distinguished impedance (a steep slope for both impedance and MET concentration) in the frequency effect (100 Hz-1 MHz) was observed at 100 Hz. When the concentration of the anti-MET antibody- colloidal gold conjugates was diluted 1009, the detectable limit for MET concentration was 1 ng/mL with 0.5 lg/mL of BSA-MET conjugate. Therefore, the developed electro-microchip is advantageous because it is effective with small sample volumes (30 lL), is a form of rapid quantitative measurement, and works with smaller detectable concentrations than other existing commercial detection prducts which have the best limit of detection of 100 ng/mL.

Original languageEnglish
Pages (from-to)319-329
Number of pages11
JournalMicrofluidics and Nanofluidics
Volume13
Issue number2
DOIs
Publication statusPublished - 2012 Sep 1

Fingerprint

methamphetamine
immunoassay
Methamphetamine
impedance measurement
Antibodies
Gold
impedance
Nanoparticles
Electrodes
Electrons
Chemical analysis
antibodies
drugs
gold
Gold Colloid
Pharmaceutical Preparations
quantitative analysis
slopes

All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Materials Chemistry

Cite this

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abstract = "In this study, an electro-microchip was successfully used to detect the impedance signals of various methamphetamine (MET) concentrations based on the developed competitive immunoassay method. MET is a commonly used drug often abused by drug addicts and can cause irregular behavior; therefore, MET concentration detection is important for quantitative analysis. In this study, gold nanoparticles (AuNPs) were introduced into an electro-microchip through the specific binding of antibodies, thus constructing a bridge between two electrodes and allowing electrons to move between them. The decreasing impedance value can be easily measured with a commercial LCR meter. According to the collected measurements, a significant difference was observed in impedance signals after 13 min when MET concentrations were reduced. Additionally, a clear, distinguished impedance (a steep slope for both impedance and MET concentration) in the frequency effect (100 Hz-1 MHz) was observed at 100 Hz. When the concentration of the anti-MET antibody- colloidal gold conjugates was diluted 1009, the detectable limit for MET concentration was 1 ng/mL with 0.5 lg/mL of BSA-MET conjugate. Therefore, the developed electro-microchip is advantageous because it is effective with small sample volumes (30 lL), is a form of rapid quantitative measurement, and works with smaller detectable concentrations than other existing commercial detection prducts which have the best limit of detection of 100 ng/mL.",
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A developed competitive immunoassay based on impedance measurements for methamphetamine detection. / Yeh, Chia Hsien; Wang, Wei Ting; Shen, Pi Lan; Lin, Yu-Cheng.

In: Microfluidics and Nanofluidics, Vol. 13, No. 2, 01.09.2012, p. 319-329.

Research output: Contribution to journalArticle

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