A disease model of muscle necrosis caused by aeromonas dhakensis infection in caenorhabditis elegans

Po Lin Chen, Yi Wei Chen, Chun Chun Ou, Tzer Min Lee, Chi Jung Wu, Wen Chien Ko, Chang Shi Chen

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

A variety of bacterial infections cause muscle necrosis in humans. Caenorhabditis elegans has epidermis and bands of muscle that resemble soft-tissue structures in mammals and humans. Here, we developed a muscle necrosis model caused by Aeromonas dhakensis infection in C. elegans. Our data showed that A. dhakensis infected and killed C. elegans rapidly. Characteristic muscle damage in C. elegans induced by A. dhakensis was demonstrated in vivo. Relative expression levels of host necrosis-associated genes, asp-3, asp-4, and crt-1 increased significantly after A. dhakensis infection. The RNAi sensitive NL2099 rrf-3 (pk1426) worms with knockdown of necrosis genes of crt-1 and asp-4 by RNAi showed prolonged survival after A. dhakensis infection. Specifically knockdown of crt-1 and asp-4 by RNAi in WM118 worms, which restricted RNAi only to the muscle cells, conferred significant resistance to A. dhakensis infection. In contrast, the severity of muscle damage and toxicity produced by the A. dhakensis hemolysin-deletion mutant is attenuated. In another example, shiga-like toxin-producing enterohemorrhagic E. coli (EHEC) known to elicit toxicity to C. elegans with concomitant enteropathogenicty, did not cause muscle necrosis as A. dhakensis did. Taken together, these results show that Aeromonas infection induces muscle necrosis and rapid death of infected C. elegans, which are similar to muscle necrosis in humans, and then validate the value of the C. elegans model with A. dhakensis infection in studying Aeromonas pathogenicity.

Original languageEnglish
Article number2058
JournalFrontiers in Microbiology
Volume7
Issue numberJAN
DOIs
Publication statusPublished - 2017 Jan 4

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Aeromonas
Caenorhabditis elegans
Necrosis
Muscles
RNA Interference
Infection
Shiga Toxins
Gene Knockdown Techniques
Enterohemorrhagic Escherichia coli
Hemolysin Proteins
Bacterial Infections
Epidermis
Muscle Cells
Virulence
Mammals
Survival

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Microbiology (medical)

Cite this

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title = "A disease model of muscle necrosis caused by aeromonas dhakensis infection in caenorhabditis elegans",
abstract = "A variety of bacterial infections cause muscle necrosis in humans. Caenorhabditis elegans has epidermis and bands of muscle that resemble soft-tissue structures in mammals and humans. Here, we developed a muscle necrosis model caused by Aeromonas dhakensis infection in C. elegans. Our data showed that A. dhakensis infected and killed C. elegans rapidly. Characteristic muscle damage in C. elegans induced by A. dhakensis was demonstrated in vivo. Relative expression levels of host necrosis-associated genes, asp-3, asp-4, and crt-1 increased significantly after A. dhakensis infection. The RNAi sensitive NL2099 rrf-3 (pk1426) worms with knockdown of necrosis genes of crt-1 and asp-4 by RNAi showed prolonged survival after A. dhakensis infection. Specifically knockdown of crt-1 and asp-4 by RNAi in WM118 worms, which restricted RNAi only to the muscle cells, conferred significant resistance to A. dhakensis infection. In contrast, the severity of muscle damage and toxicity produced by the A. dhakensis hemolysin-deletion mutant is attenuated. In another example, shiga-like toxin-producing enterohemorrhagic E. coli (EHEC) known to elicit toxicity to C. elegans with concomitant enteropathogenicty, did not cause muscle necrosis as A. dhakensis did. Taken together, these results show that Aeromonas infection induces muscle necrosis and rapid death of infected C. elegans, which are similar to muscle necrosis in humans, and then validate the value of the C. elegans model with A. dhakensis infection in studying Aeromonas pathogenicity.",
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A disease model of muscle necrosis caused by aeromonas dhakensis infection in caenorhabditis elegans. / Chen, Po Lin; Chen, Yi Wei; Ou, Chun Chun; Lee, Tzer Min; Wu, Chi Jung; Ko, Wen Chien; Chen, Chang Shi.

In: Frontiers in Microbiology, Vol. 7, No. JAN, 2058, 04.01.2017.

Research output: Contribution to journalArticle

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AU - Chen, Yi Wei

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