A DNA hybridization assay using nanogold-streptavidin, electro-microchip and silver enhancement to analyze impedance signals

Chia Hsien Yeh, Yu Huai Chang, Hong Ping Lin, Tsung Chain Chang, Yu Cheng Lin

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

This paper reports a novel DNA hybridization detection method using gold-streptavidin conjugates as a reporter, the silver enhancement reaction to magnify the detection signal, a commercial LCR meter to detect the hybridization signal. The relationships between sample concentration and detection signal are discussed and the detection limit for the DNA sample is 0.825 ng/mL. Moreover, we discuss the probe specific test for different species of bacteria, for same species but different strains, and for same genus but different species.

Original languageEnglish
Title of host publicationProceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences
PublisherChemical and Biological Microsystems Society
Pages1004-1007
Number of pages4
ISBN (Print)9780979806421
Publication statusPublished - 2009 Jan 1
Event13th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2009 - Jeju, Korea, Republic of
Duration: 2009 Nov 12009 Nov 5

Publication series

NameProceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences

Other

Other13th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2009
CountryKorea, Republic of
CityJeju
Period09-11-0109-11-05

All Science Journal Classification (ASJC) codes

  • Chemical Engineering (miscellaneous)
  • Bioengineering

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  • Cite this

    Yeh, C. H., Chang, Y. H., Lin, H. P., Chang, T. C., & Lin, Y. C. (2009). A DNA hybridization assay using nanogold-streptavidin, electro-microchip and silver enhancement to analyze impedance signals. In Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences (pp. 1004-1007). (Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences). Chemical and Biological Microsystems Society.