TY - GEN
T1 - A DNA hybridization assay using nanogold-streptavidin, electro-microchip and silver enhancement to analyze impedance signals
AU - Yeh, Chia Hsien
AU - Chang, Yu Huai
AU - Lin, Hong Ping
AU - Chang, Tsung Chain
AU - Lin, Yu Cheng
PY - 2009/1/1
Y1 - 2009/1/1
N2 - This paper reports a novel DNA hybridization detection method using gold-streptavidin conjugates as a reporter, the silver enhancement reaction to magnify the detection signal, a commercial LCR meter to detect the hybridization signal. The relationships between sample concentration and detection signal are discussed and the detection limit for the DNA sample is 0.825 ng/mL. Moreover, we discuss the probe specific test for different species of bacteria, for same species but different strains, and for same genus but different species.
AB - This paper reports a novel DNA hybridization detection method using gold-streptavidin conjugates as a reporter, the silver enhancement reaction to magnify the detection signal, a commercial LCR meter to detect the hybridization signal. The relationships between sample concentration and detection signal are discussed and the detection limit for the DNA sample is 0.825 ng/mL. Moreover, we discuss the probe specific test for different species of bacteria, for same species but different strains, and for same genus but different species.
UR - http://www.scopus.com/inward/record.url?scp=84901799202&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84901799202&partnerID=8YFLogxK
M3 - Conference contribution
AN - SCOPUS:84901799202
SN - 9780979806421
T3 - Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences
SP - 1004
EP - 1007
BT - Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences
PB - Chemical and Biological Microsystems Society
T2 - 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2009
Y2 - 1 November 2009 through 5 November 2009
ER -