A DNA hybridization assay using nanogold-streptavidin, electro-microchip and silver enhancement to analyze impedance signals

Chen-Sheng Yeh, Y. H. Chang, Hong-Ping Lin, T. C. Chang, Yu-Cheng Lin

Research output: Contribution to journalConference article

1 Citation (Scopus)

Abstract

This paper reports a novel DNA hybridization detection method using gold-streptavidin conjugates as a reporter, the silver enhancement reaction to magnify the detection signal, a commercial LCR meter to detect the hybridization signal. The relationships between sample concentration and detection signal are discussed and the detection limit for the DNA sample is 0.825 ng/mL. Moreover, the probe specific tests are discussed for different species of bacteria, for same species but different strains, and for same genus but different species.

Original languageEnglish
Pages (from-to)333-336
Number of pages4
JournalProcedia Chemistry
Volume1
Issue number1
DOIs
Publication statusPublished - 2009 Sep 1
EventEurosensors 23rd Conference - Lausanne, Switzerland
Duration: 2009 Sep 62009 Sep 9

Fingerprint

Streptavidin
Signal detection
Silver
Assays
DNA
Gold
Bacteria

All Science Journal Classification (ASJC) codes

  • Chemistry(all)
  • Chemical Engineering(all)

Cite this

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abstract = "This paper reports a novel DNA hybridization detection method using gold-streptavidin conjugates as a reporter, the silver enhancement reaction to magnify the detection signal, a commercial LCR meter to detect the hybridization signal. The relationships between sample concentration and detection signal are discussed and the detection limit for the DNA sample is 0.825 ng/mL. Moreover, the probe specific tests are discussed for different species of bacteria, for same species but different strains, and for same genus but different species.",
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A DNA hybridization assay using nanogold-streptavidin, electro-microchip and silver enhancement to analyze impedance signals. / Yeh, Chen-Sheng; Chang, Y. H.; Lin, Hong-Ping; Chang, T. C.; Lin, Yu-Cheng.

In: Procedia Chemistry, Vol. 1, No. 1, 01.09.2009, p. 333-336.

Research output: Contribution to journalConference article

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AU - Chang, Y. H.

AU - Lin, Hong-Ping

AU - Chang, T. C.

AU - Lin, Yu-Cheng

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