A feasible and practical 1H NMR analytical method for the quality control and quantification of bioactive principles in Lycii Fructus

Li Ying Hsieh, Hsiu Hui Chan, Ping-Chung Kuo, Hsin-Yi Hung, Yue Chiun Li, Chao Lin Kuo, Yong Peng, Zhong Zhen Zhao, Daih Huang Kuo, I-Wen Sun, Tian-Shung Wu

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Lycii Fructus, a solanaceous drug, is widely used as functional foods and in Traditional Chinese Medicine. Samples collected from different regions of China have been found to be not identical in chemical compositions which might affect the biological activities. Although many chromatographic and spectrometric methods have been reported to determine the concentration of betaine and other bioactive amino acids, disturbance resulted from other polar substances with low UV-absorbance and expensive mass facilities reduced the applicability of these techniques. In the present study, the strong cation exchange solid phase extraction procedure incorporated with 1H NMR was successfully developed as a rapid and reliable method that can simultaneously determine betaine, citric acid, threonine, alanine, and proline in various Lycii Fructus. In addition, ERETIC 2 method based on PULCON principle was also applied and compared with conventional method. This feasible and practical method offers a very powerful tool for the quality control of commercial Lycii Fructus from different sources.

Original languageEnglish
Pages (from-to)1105-1112
Number of pages8
JournalJournal of Food and Drug Analysis
Volume26
Issue number3
DOIs
Publication statusPublished - 2018 Jul 1

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Quality Control
quality control
analytical methods
Betaine
betaine
methodology
Functional Food
cation exchange
Solid Phase Extraction
Chinese Traditional Medicine
Threonine
solid phase extraction
Proline
threonine
Citric Acid
citric acid
Alanine
alanine
functional foods
absorbance

All Science Journal Classification (ASJC) codes

  • Food Science
  • Pharmacology

Cite this

Hsieh, Li Ying ; Chan, Hsiu Hui ; Kuo, Ping-Chung ; Hung, Hsin-Yi ; Li, Yue Chiun ; Kuo, Chao Lin ; Peng, Yong ; Zhao, Zhong Zhen ; Kuo, Daih Huang ; Sun, I-Wen ; Wu, Tian-Shung. / A feasible and practical 1H NMR analytical method for the quality control and quantification of bioactive principles in Lycii Fructus. In: Journal of Food and Drug Analysis. 2018 ; Vol. 26, No. 3. pp. 1105-1112.
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A feasible and practical 1H NMR analytical method for the quality control and quantification of bioactive principles in Lycii Fructus. / Hsieh, Li Ying; Chan, Hsiu Hui; Kuo, Ping-Chung; Hung, Hsin-Yi; Li, Yue Chiun; Kuo, Chao Lin; Peng, Yong; Zhao, Zhong Zhen; Kuo, Daih Huang; Sun, I-Wen; Wu, Tian-Shung.

In: Journal of Food and Drug Analysis, Vol. 26, No. 3, 01.07.2018, p. 1105-1112.

Research output: Contribution to journalArticle

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AU - Hsieh, Li Ying

AU - Chan, Hsiu Hui

AU - Kuo, Ping-Chung

AU - Hung, Hsin-Yi

AU - Li, Yue Chiun

AU - Kuo, Chao Lin

AU - Peng, Yong

AU - Zhao, Zhong Zhen

AU - Kuo, Daih Huang

AU - Sun, I-Wen

AU - Wu, Tian-Shung

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