A feasible and practical 1H NMR analytical method for the quality control and quantification of bioactive principles in Lycii Fructus

Li Ying Hsieh, Hsiu Hui Chan, Ping Chung Kuo, Hsin Yi Hung, Yue Chiun Li, Chao Lin Kuo, Yong Peng, Zhong Zhen Zhao, Daih Huang Kuo, I. Wen Sun, Tian Shung Wu

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Lycii Fructus, a solanaceous drug, is widely used as functional foods and in Traditional Chinese Medicine. Samples collected from different regions of China have been found to be not identical in chemical compositions which might affect the biological activities. Although many chromatographic and spectrometric methods have been reported to determine the concentration of betaine and other bioactive amino acids, disturbance resulted from other polar substances with low UV-absorbance and expensive mass facilities reduced the applicability of these techniques. In the present study, the strong cation exchange solid phase extraction procedure incorporated with 1H NMR was successfully developed as a rapid and reliable method that can simultaneously determine betaine, citric acid, threonine, alanine, and proline in various Lycii Fructus. In addition, ERETIC 2 method based on PULCON principle was also applied and compared with conventional method. This feasible and practical method offers a very powerful tool for the quality control of commercial Lycii Fructus from different sources.

Original languageEnglish
Pages (from-to)1105-1112
Number of pages8
JournalJournal of Food and Drug Analysis
Volume26
Issue number3
DOIs
Publication statusPublished - 2018 Jul

Fingerprint

Quality Control
quality control
analytical methods
Betaine
betaine
methodology
Functional Food
cation exchange
Solid Phase Extraction
Chinese Traditional Medicine
Threonine
solid phase extraction
Proline
threonine
Citric Acid
citric acid
Alanine
alanine
functional foods
absorbance

All Science Journal Classification (ASJC) codes

  • Food Science
  • Pharmacology

Cite this

Hsieh, Li Ying ; Chan, Hsiu Hui ; Kuo, Ping Chung ; Hung, Hsin Yi ; Li, Yue Chiun ; Kuo, Chao Lin ; Peng, Yong ; Zhao, Zhong Zhen ; Kuo, Daih Huang ; Sun, I. Wen ; Wu, Tian Shung. / A feasible and practical 1H NMR analytical method for the quality control and quantification of bioactive principles in Lycii Fructus. In: Journal of Food and Drug Analysis. 2018 ; Vol. 26, No. 3. pp. 1105-1112.
@article{62577d9499bf4cbca3e0cb877a06842f,
title = "A feasible and practical 1H NMR analytical method for the quality control and quantification of bioactive principles in Lycii Fructus",
abstract = "Lycii Fructus, a solanaceous drug, is widely used as functional foods and in Traditional Chinese Medicine. Samples collected from different regions of China have been found to be not identical in chemical compositions which might affect the biological activities. Although many chromatographic and spectrometric methods have been reported to determine the concentration of betaine and other bioactive amino acids, disturbance resulted from other polar substances with low UV-absorbance and expensive mass facilities reduced the applicability of these techniques. In the present study, the strong cation exchange solid phase extraction procedure incorporated with 1H NMR was successfully developed as a rapid and reliable method that can simultaneously determine betaine, citric acid, threonine, alanine, and proline in various Lycii Fructus. In addition, ERETIC 2 method based on PULCON principle was also applied and compared with conventional method. This feasible and practical method offers a very powerful tool for the quality control of commercial Lycii Fructus from different sources.",
author = "Hsieh, {Li Ying} and Chan, {Hsiu Hui} and Kuo, {Ping Chung} and Hung, {Hsin Yi} and Li, {Yue Chiun} and Kuo, {Chao Lin} and Yong Peng and Zhao, {Zhong Zhen} and Kuo, {Daih Huang} and Sun, {I. Wen} and Wu, {Tian Shung}",
year = "2018",
month = "7",
doi = "10.1016/j.jfda.2018.01.001",
language = "English",
volume = "26",
pages = "1105--1112",
journal = "Journal of Food and Drug Analysis",
issn = "1021-9498",
publisher = "National Laboratories of Foods and Drugs",
number = "3",

}

A feasible and practical 1H NMR analytical method for the quality control and quantification of bioactive principles in Lycii Fructus. / Hsieh, Li Ying; Chan, Hsiu Hui; Kuo, Ping Chung; Hung, Hsin Yi; Li, Yue Chiun; Kuo, Chao Lin; Peng, Yong; Zhao, Zhong Zhen; Kuo, Daih Huang; Sun, I. Wen; Wu, Tian Shung.

In: Journal of Food and Drug Analysis, Vol. 26, No. 3, 07.2018, p. 1105-1112.

Research output: Contribution to journalArticle

TY - JOUR

T1 - A feasible and practical 1H NMR analytical method for the quality control and quantification of bioactive principles in Lycii Fructus

AU - Hsieh, Li Ying

AU - Chan, Hsiu Hui

AU - Kuo, Ping Chung

AU - Hung, Hsin Yi

AU - Li, Yue Chiun

AU - Kuo, Chao Lin

AU - Peng, Yong

AU - Zhao, Zhong Zhen

AU - Kuo, Daih Huang

AU - Sun, I. Wen

AU - Wu, Tian Shung

PY - 2018/7

Y1 - 2018/7

N2 - Lycii Fructus, a solanaceous drug, is widely used as functional foods and in Traditional Chinese Medicine. Samples collected from different regions of China have been found to be not identical in chemical compositions which might affect the biological activities. Although many chromatographic and spectrometric methods have been reported to determine the concentration of betaine and other bioactive amino acids, disturbance resulted from other polar substances with low UV-absorbance and expensive mass facilities reduced the applicability of these techniques. In the present study, the strong cation exchange solid phase extraction procedure incorporated with 1H NMR was successfully developed as a rapid and reliable method that can simultaneously determine betaine, citric acid, threonine, alanine, and proline in various Lycii Fructus. In addition, ERETIC 2 method based on PULCON principle was also applied and compared with conventional method. This feasible and practical method offers a very powerful tool for the quality control of commercial Lycii Fructus from different sources.

AB - Lycii Fructus, a solanaceous drug, is widely used as functional foods and in Traditional Chinese Medicine. Samples collected from different regions of China have been found to be not identical in chemical compositions which might affect the biological activities. Although many chromatographic and spectrometric methods have been reported to determine the concentration of betaine and other bioactive amino acids, disturbance resulted from other polar substances with low UV-absorbance and expensive mass facilities reduced the applicability of these techniques. In the present study, the strong cation exchange solid phase extraction procedure incorporated with 1H NMR was successfully developed as a rapid and reliable method that can simultaneously determine betaine, citric acid, threonine, alanine, and proline in various Lycii Fructus. In addition, ERETIC 2 method based on PULCON principle was also applied and compared with conventional method. This feasible and practical method offers a very powerful tool for the quality control of commercial Lycii Fructus from different sources.

UR - http://www.scopus.com/inward/record.url?scp=85041172842&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85041172842&partnerID=8YFLogxK

U2 - 10.1016/j.jfda.2018.01.001

DO - 10.1016/j.jfda.2018.01.001

M3 - Article

C2 - 29976403

AN - SCOPUS:85041172842

VL - 26

SP - 1105

EP - 1112

JO - Journal of Food and Drug Analysis

JF - Journal of Food and Drug Analysis

SN - 1021-9498

IS - 3

ER -