A fibrinogen assay using specific aptamer on an integrated microfluidic chip

Yi Da Chung; Anirban Sinha; Priya Gopinathan; Hsin Ying Lin; Shu Chu Shiesh; Gwo Bin Lee

A fibrinogen assay using specific aptamer on an integrated microfluidic chip

Yi Da Chung, Anirban Sinha, Priya Gopinathan, Hsin Ying Lin, Shu Chu Shiesh, Gwo Bin Lee

Department of Medical Laboratory Science and Biotechnology

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

Fibrinogen is a risk factor and useful biomarker for cardiovascular diseases (CVD). We selected single stranded DNA aptamers as new-affinity reagent which can specifically bind to fibrinogen. An integrated microfluidic chip by using magnetic beads coated with aptamer for detecting fibrinogen was reported. Instead of using antibodies, an aptamer was used as a capturing probe toward fibrinogen. The aptamer has been verified its affinity to fibrinogen. The experimentally data showed that this integrated microfluidic chip can assay fibrinogen concentrations with only few microliter plasma samples.

Original language	English
Title of host publication	22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018
Publisher	Chemical and Biological Microsystems Society
Pages	1882-1885
Number of pages	4
ISBN (Electronic)	9781510897571
Publication status	Published - 2018 Jan 1
Event	22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018 - Kaohsiung, Taiwan Duration: 2018 Nov 11 → 2018 Nov 15

Publication series

Name	22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018
Volume	4

Conference

Conference	22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018
Country	Taiwan
City	Kaohsiung
Period	18-11-11 → 18-11-15

All Science Journal Classification (ASJC) codes

Chemistry(all)
Bioengineering
Chemical Engineering (miscellaneous)
Control and Systems Engineering

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Chung, Y. D., Sinha, A., Gopinathan, P., Lin, H. Y., Shiesh, S. C., & Lee, G. B. (2018). A fibrinogen assay using specific aptamer on an integrated microfluidic chip. In 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018 (pp. 1882-1885). (22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018; Vol. 4). Chemical and Biological Microsystems Society.

Chung, Yi Da ; Sinha, Anirban ; Gopinathan, Priya ; Lin, Hsin Ying ; Shiesh, Shu Chu ; Lee, Gwo Bin. / A fibrinogen assay using specific aptamer on an integrated microfluidic chip. 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018. Chemical and Biological Microsystems Society, 2018. pp. 1882-1885 (22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018).

@inproceedings{7c0c11d3f71b4144a1f711179b524942,

title = "A fibrinogen assay using specific aptamer on an integrated microfluidic chip",

abstract = "Fibrinogen is a risk factor and useful biomarker for cardiovascular diseases (CVD). We selected single stranded DNA aptamers as new-affinity reagent which can specifically bind to fibrinogen. An integrated microfluidic chip by using magnetic beads coated with aptamer for detecting fibrinogen was reported. Instead of using antibodies, an aptamer was used as a capturing probe toward fibrinogen. The aptamer has been verified its affinity to fibrinogen. The experimentally data showed that this integrated microfluidic chip can assay fibrinogen concentrations with only few microliter plasma samples.",

author = "Chung, {Yi Da} and Anirban Sinha and Priya Gopinathan and Lin, {Hsin Ying} and Shiesh, {Shu Chu} and Lee, {Gwo Bin}",

year = "2018",

month = jan,

day = "1",

language = "English",

series = "22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018",

publisher = "Chemical and Biological Microsystems Society",

pages = "1882--1885",

booktitle = "22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018",

note = "22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018 ; Conference date: 11-11-2018 Through 15-11-2018",

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Chung, YD, Sinha, A, Gopinathan, P, Lin, HY, Shiesh, SC & Lee, GB 2018, A fibrinogen assay using specific aptamer on an integrated microfluidic chip. in 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018. 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018, vol. 4, Chemical and Biological Microsystems Society, pp. 1882-1885, 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018, Kaohsiung, Taiwan, 18-11-11.

A fibrinogen assay using specific aptamer on an integrated microfluidic chip. / Chung, Yi Da; Sinha, Anirban; Gopinathan, Priya; Lin, Hsin Ying; Shiesh, Shu Chu; Lee, Gwo Bin.

22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018. Chemical and Biological Microsystems Society, 2018. p. 1882-1885 (22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018; Vol. 4).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

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AU - Lee, Gwo Bin

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N2 - Fibrinogen is a risk factor and useful biomarker for cardiovascular diseases (CVD). We selected single stranded DNA aptamers as new-affinity reagent which can specifically bind to fibrinogen. An integrated microfluidic chip by using magnetic beads coated with aptamer for detecting fibrinogen was reported. Instead of using antibodies, an aptamer was used as a capturing probe toward fibrinogen. The aptamer has been verified its affinity to fibrinogen. The experimentally data showed that this integrated microfluidic chip can assay fibrinogen concentrations with only few microliter plasma samples.

AB - Fibrinogen is a risk factor and useful biomarker for cardiovascular diseases (CVD). We selected single stranded DNA aptamers as new-affinity reagent which can specifically bind to fibrinogen. An integrated microfluidic chip by using magnetic beads coated with aptamer for detecting fibrinogen was reported. Instead of using antibodies, an aptamer was used as a capturing probe toward fibrinogen. The aptamer has been verified its affinity to fibrinogen. The experimentally data showed that this integrated microfluidic chip can assay fibrinogen concentrations with only few microliter plasma samples.

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Chung YD, Sinha A, Gopinathan P, Lin HY, Shiesh SC, Lee GB. A fibrinogen assay using specific aptamer on an integrated microfluidic chip. In 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018. Chemical and Biological Microsystems Society. 2018. p. 1882-1885. (22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018).