A high-discernment microflow cytometer with microweir structure

Lung Ming Fu, Chien Hsiung Tsai, Che Hsin Lin

Research output: Contribution to journalArticle

42 Citations (Scopus)


Using a simple and reliable isotropic wet etching process, we fabricated a microflow cytometer in which cells/particles are concentrated in the center ofthe sample stream using a 2-D hydrodynamic focusing technique and a microweir structure. Having focused the cells/particles, they are detected and counted using an LIF method. The experimental and numerical results confirm the effectiveness of the hydrodynamic sheath flows in squeezing the cells/particles into a narrow stream in the horizontal X-Y plane. Furthermore, it is shown numerically that the microweir structure results in the separation of the cells/particles in the vertical X-Z plane such that they pass through the detection region in a sequential fashion and can therefore be counted with a high degree of precision. The experimental results obtained using fluorescent polystyrene beads with diameters of 5 and 10 μm, respectively, confirm the suitability of the proposed device for microfluidic applications requiring the high-precision counting of particles or cells within a sample flow.

Original languageEnglish
Pages (from-to)1874-1880
Number of pages7
Issue number9
Publication statusPublished - 2008 May 1

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Biochemistry
  • Clinical Biochemistry

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