TY - GEN
T1 - A lab-use microfluidic planar patch-clamp system
AU - Tu, Ting Yuan
AU - Chen, Chang Yu
AU - Jong, De Shien
AU - Wo, Andrew M.
PY - 2010
Y1 - 2010
N2 - This work presents a microfluidic planar patch-clamp system suitable for laboratory usage without high entry threshold as the traditional pipette technique. Our previously developed hourglass-shaped aperture integrated with simple mi-crofluidic system available for whole-cell recording on HIT-T15, CHO-K1, and HEK-293T cell lines. Endogenous outward rectifier and A-type potassium currents were measured from HEK-293T. Extracellular solution are exchangeable up to six different solutions for ion channel interrogation. The fastest extracellular solution exchange rate demonstrated on HEK cells was within 500ms. The lab-use microfluidic planar patch-clamp system is believed to serve as a practical mi-crodevice for general laboratory usage.
AB - This work presents a microfluidic planar patch-clamp system suitable for laboratory usage without high entry threshold as the traditional pipette technique. Our previously developed hourglass-shaped aperture integrated with simple mi-crofluidic system available for whole-cell recording on HIT-T15, CHO-K1, and HEK-293T cell lines. Endogenous outward rectifier and A-type potassium currents were measured from HEK-293T. Extracellular solution are exchangeable up to six different solutions for ion channel interrogation. The fastest extracellular solution exchange rate demonstrated on HEK cells was within 500ms. The lab-use microfluidic planar patch-clamp system is believed to serve as a practical mi-crodevice for general laboratory usage.
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M3 - Conference contribution
AN - SCOPUS:84884408324
SN - 9781618390622
T3 - 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010
SP - 929
EP - 931
BT - 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010
T2 - 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010
Y2 - 3 October 2010 through 7 October 2010
ER -