A lab-use microfluidic planar patch-clamp system

Ting-Yuan Tu, Chang Yu Chen, De Shien Jong, Andrew M. Wo

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

This work presents a microfluidic planar patch-clamp system suitable for laboratory usage without high entry threshold as the traditional pipette technique. Our previously developed hourglass-shaped aperture integrated with simple mi-crofluidic system available for whole-cell recording on HIT-T15, CHO-K1, and HEK-293T cell lines. Endogenous outward rectifier and A-type potassium currents were measured from HEK-293T. Extracellular solution are exchangeable up to six different solutions for ion channel interrogation. The fastest extracellular solution exchange rate demonstrated on HEK cells was within 500ms. The lab-use microfluidic planar patch-clamp system is believed to serve as a practical mi-crodevice for general laboratory usage.

Original languageEnglish
Title of host publication14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010
Pages929-931
Number of pages3
Publication statusPublished - 2010 Dec 1
Event14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010 - Groningen, Netherlands
Duration: 2010 Oct 32010 Oct 7

Publication series

Name14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010
Volume2

Other

Other14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010
CountryNetherlands
CityGroningen
Period10-10-0310-10-07

Fingerprint

Clamping devices
Microfluidics
Potassium
Ion exchange
Cells
Ions

All Science Journal Classification (ASJC) codes

  • Control and Systems Engineering

Cite this

Tu, T-Y., Chen, C. Y., Jong, D. S., & Wo, A. M. (2010). A lab-use microfluidic planar patch-clamp system. In 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010 (pp. 929-931). (14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010; Vol. 2).
Tu, Ting-Yuan ; Chen, Chang Yu ; Jong, De Shien ; Wo, Andrew M. / A lab-use microfluidic planar patch-clamp system. 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010. 2010. pp. 929-931 (14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010).
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Tu, T-Y, Chen, CY, Jong, DS & Wo, AM 2010, A lab-use microfluidic planar patch-clamp system. in 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010. 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010, vol. 2, pp. 929-931, 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010, Groningen, Netherlands, 10-10-03.

A lab-use microfluidic planar patch-clamp system. / Tu, Ting-Yuan; Chen, Chang Yu; Jong, De Shien; Wo, Andrew M.

14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010. 2010. p. 929-931 (14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010; Vol. 2).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

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Tu T-Y, Chen CY, Jong DS, Wo AM. A lab-use microfluidic planar patch-clamp system. In 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010. 2010. p. 929-931. (14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010).