A magnetic bead-based three-dimensional micro-incubator for rapid purification and detection of tumor cells

Kang Yi Lien, Chien Ju Liu, Keng Fu Hsu, Wu Wei Lai, Cheng Yang Chou, Gwo Bin Lee

Research output: Chapter in Book/Report/Conference proceedingConference contribution

3 Citations (Scopus)

Abstract

The current study presents an innovative magnetic bead-based 3-dimensional (3D) micro-incubator for rapid purification of tumor cells (TCs) by using micro-electro-mechanical-systems (MEMS) technologies. With the incorporation of magnetic beads, specific antibodies conjugated onto the surface of the magnetic beads can be used to specifically recognize the TCs in the clinical body fluids, followed by collecting and purifying them with the incorporation of a magnet and built-in microfluidic control module. The novel 3D micro-incubator was developed for incubation and mixing process in the purification process when a relatively large amount of bio-sample was required. The vortex effect can be generated within the incubation chamber such that large amount of clinical bio-samples (∼ 1000 μl) can be mixed efficiently within a short period of time (less than 10 minutes). In addition, successful demonstration for detection of ovarian and lung cancer cells has been achieved by performing an on-chip genetic identification. As a result, magnetic bead-based 3D micro-incubator integrated with microfluidic modules may provide an automatic platform for rapid purification and fast detection of cancer cells.

Original languageEnglish
Title of host publicationMEMS 2010 - The 23rd IEEE International Conference on Micro Electro Mechanical Systems, Technical Digest
Pages100-103
Number of pages4
DOIs
Publication statusPublished - 2010 Jun 1
Event23rd IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2010 - Hong Kong, China
Duration: 2010 Jan 242010 Jan 28

Publication series

NameProceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)
ISSN (Print)1084-6999

Other

Other23rd IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2010
CountryChina
CityHong Kong
Period10-01-2410-01-28

Fingerprint

purification
beads
Purification
Tumors
tumors
Cells
Microfluidics
modules
cancer
Body fluids
Antibodies
Magnets
body fluids
Vortex flow
Demonstrations
antibodies
lungs
magnets
platforms
chambers

All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Mechanical Engineering
  • Electrical and Electronic Engineering

Cite this

Lien, K. Y., Liu, C. J., Hsu, K. F., Lai, W. W., Chou, C. Y., & Lee, G. B. (2010). A magnetic bead-based three-dimensional micro-incubator for rapid purification and detection of tumor cells. In MEMS 2010 - The 23rd IEEE International Conference on Micro Electro Mechanical Systems, Technical Digest (pp. 100-103). [5442557] (Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)). https://doi.org/10.1109/MEMSYS.2010.5442557
Lien, Kang Yi ; Liu, Chien Ju ; Hsu, Keng Fu ; Lai, Wu Wei ; Chou, Cheng Yang ; Lee, Gwo Bin. / A magnetic bead-based three-dimensional micro-incubator for rapid purification and detection of tumor cells. MEMS 2010 - The 23rd IEEE International Conference on Micro Electro Mechanical Systems, Technical Digest. 2010. pp. 100-103 (Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)).
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title = "A magnetic bead-based three-dimensional micro-incubator for rapid purification and detection of tumor cells",
abstract = "The current study presents an innovative magnetic bead-based 3-dimensional (3D) micro-incubator for rapid purification of tumor cells (TCs) by using micro-electro-mechanical-systems (MEMS) technologies. With the incorporation of magnetic beads, specific antibodies conjugated onto the surface of the magnetic beads can be used to specifically recognize the TCs in the clinical body fluids, followed by collecting and purifying them with the incorporation of a magnet and built-in microfluidic control module. The novel 3D micro-incubator was developed for incubation and mixing process in the purification process when a relatively large amount of bio-sample was required. The vortex effect can be generated within the incubation chamber such that large amount of clinical bio-samples (∼ 1000 μl) can be mixed efficiently within a short period of time (less than 10 minutes). In addition, successful demonstration for detection of ovarian and lung cancer cells has been achieved by performing an on-chip genetic identification. As a result, magnetic bead-based 3D micro-incubator integrated with microfluidic modules may provide an automatic platform for rapid purification and fast detection of cancer cells.",
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Lien, KY, Liu, CJ, Hsu, KF, Lai, WW, Chou, CY & Lee, GB 2010, A magnetic bead-based three-dimensional micro-incubator for rapid purification and detection of tumor cells. in MEMS 2010 - The 23rd IEEE International Conference on Micro Electro Mechanical Systems, Technical Digest., 5442557, Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS), pp. 100-103, 23rd IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2010, Hong Kong, China, 10-01-24. https://doi.org/10.1109/MEMSYS.2010.5442557

A magnetic bead-based three-dimensional micro-incubator for rapid purification and detection of tumor cells. / Lien, Kang Yi; Liu, Chien Ju; Hsu, Keng Fu; Lai, Wu Wei; Chou, Cheng Yang; Lee, Gwo Bin.

MEMS 2010 - The 23rd IEEE International Conference on Micro Electro Mechanical Systems, Technical Digest. 2010. p. 100-103 5442557 (Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

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AU - Lee, Gwo Bin

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N2 - The current study presents an innovative magnetic bead-based 3-dimensional (3D) micro-incubator for rapid purification of tumor cells (TCs) by using micro-electro-mechanical-systems (MEMS) technologies. With the incorporation of magnetic beads, specific antibodies conjugated onto the surface of the magnetic beads can be used to specifically recognize the TCs in the clinical body fluids, followed by collecting and purifying them with the incorporation of a magnet and built-in microfluidic control module. The novel 3D micro-incubator was developed for incubation and mixing process in the purification process when a relatively large amount of bio-sample was required. The vortex effect can be generated within the incubation chamber such that large amount of clinical bio-samples (∼ 1000 μl) can be mixed efficiently within a short period of time (less than 10 minutes). In addition, successful demonstration for detection of ovarian and lung cancer cells has been achieved by performing an on-chip genetic identification. As a result, magnetic bead-based 3D micro-incubator integrated with microfluidic modules may provide an automatic platform for rapid purification and fast detection of cancer cells.

AB - The current study presents an innovative magnetic bead-based 3-dimensional (3D) micro-incubator for rapid purification of tumor cells (TCs) by using micro-electro-mechanical-systems (MEMS) technologies. With the incorporation of magnetic beads, specific antibodies conjugated onto the surface of the magnetic beads can be used to specifically recognize the TCs in the clinical body fluids, followed by collecting and purifying them with the incorporation of a magnet and built-in microfluidic control module. The novel 3D micro-incubator was developed for incubation and mixing process in the purification process when a relatively large amount of bio-sample was required. The vortex effect can be generated within the incubation chamber such that large amount of clinical bio-samples (∼ 1000 μl) can be mixed efficiently within a short period of time (less than 10 minutes). In addition, successful demonstration for detection of ovarian and lung cancer cells has been achieved by performing an on-chip genetic identification. As a result, magnetic bead-based 3D micro-incubator integrated with microfluidic modules may provide an automatic platform for rapid purification and fast detection of cancer cells.

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T3 - Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)

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BT - MEMS 2010 - The 23rd IEEE International Conference on Micro Electro Mechanical Systems, Technical Digest

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Lien KY, Liu CJ, Hsu KF, Lai WW, Chou CY, Lee GB. A magnetic bead-based three-dimensional micro-incubator for rapid purification and detection of tumor cells. In MEMS 2010 - The 23rd IEEE International Conference on Micro Electro Mechanical Systems, Technical Digest. 2010. p. 100-103. 5442557. (Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)). https://doi.org/10.1109/MEMSYS.2010.5442557