TY - GEN
T1 - A magnetic bead-based three-dimensional micro-incubator for rapid purification and detection of tumor cells
AU - Lien, Kang Yi
AU - Liu, Chien Ju
AU - Hsu, Keng Fu
AU - Lai, Wu Wei
AU - Chou, Cheng Yang
AU - Lee, Gwo Bin
PY - 2010/6/1
Y1 - 2010/6/1
N2 - The current study presents an innovative magnetic bead-based 3-dimensional (3D) micro-incubator for rapid purification of tumor cells (TCs) by using micro-electro-mechanical-systems (MEMS) technologies. With the incorporation of magnetic beads, specific antibodies conjugated onto the surface of the magnetic beads can be used to specifically recognize the TCs in the clinical body fluids, followed by collecting and purifying them with the incorporation of a magnet and built-in microfluidic control module. The novel 3D micro-incubator was developed for incubation and mixing process in the purification process when a relatively large amount of bio-sample was required. The vortex effect can be generated within the incubation chamber such that large amount of clinical bio-samples (∼ 1000 μl) can be mixed efficiently within a short period of time (less than 10 minutes). In addition, successful demonstration for detection of ovarian and lung cancer cells has been achieved by performing an on-chip genetic identification. As a result, magnetic bead-based 3D micro-incubator integrated with microfluidic modules may provide an automatic platform for rapid purification and fast detection of cancer cells.
AB - The current study presents an innovative magnetic bead-based 3-dimensional (3D) micro-incubator for rapid purification of tumor cells (TCs) by using micro-electro-mechanical-systems (MEMS) technologies. With the incorporation of magnetic beads, specific antibodies conjugated onto the surface of the magnetic beads can be used to specifically recognize the TCs in the clinical body fluids, followed by collecting and purifying them with the incorporation of a magnet and built-in microfluidic control module. The novel 3D micro-incubator was developed for incubation and mixing process in the purification process when a relatively large amount of bio-sample was required. The vortex effect can be generated within the incubation chamber such that large amount of clinical bio-samples (∼ 1000 μl) can be mixed efficiently within a short period of time (less than 10 minutes). In addition, successful demonstration for detection of ovarian and lung cancer cells has been achieved by performing an on-chip genetic identification. As a result, magnetic bead-based 3D micro-incubator integrated with microfluidic modules may provide an automatic platform for rapid purification and fast detection of cancer cells.
UR - http://www.scopus.com/inward/record.url?scp=77952778328&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=77952778328&partnerID=8YFLogxK
U2 - 10.1109/MEMSYS.2010.5442557
DO - 10.1109/MEMSYS.2010.5442557
M3 - Conference contribution
AN - SCOPUS:77952778328
SN - 9781424457649
T3 - Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)
SP - 100
EP - 103
BT - MEMS 2010 - The 23rd IEEE International Conference on Micro Electro Mechanical Systems, Technical Digest
T2 - 23rd IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2010
Y2 - 24 January 2010 through 28 January 2010
ER -
