A member of the immunoglobulin superfamily, orange-spotted grouper novel immune gene EcVig, is induced by immune stimulants and type I interferon

Ying Chun Yeh, Ting Yu Wang, Hsin Yiu Chou, Han You Lin, Tzong Yueh Chen, Takashi Aoki, Han Ching Wang

Research output: Contribution to journalArticle

Abstract

A novel grouper immune gene, EcVig was identified in orange-spotted grouper (Epinephelus coioides). We recently determined that EcVig expression can be induced by infection with nervous necrosis virus (NNV, an RNA virus), whereas NNV replication may be suppressed when EcVig was overexpressed. Although EcVig appeared to be involved in grouper antiviral activity, its immune effects have not been well characterized. In the present study, two PAMPs (pathogen-associated molecular patterns; lipopolysaccharides [LPS] and synthetic double-stranded RNA polyriboinosinic-polyribocytidylic acid [poly(I:C)]), as well as fish DNA virus (red sea bream iridovirus, RSIV; grouper iridovirus, GIV), were used to study EcVig responses in orange-spotted grouper. In addition, groupers were given recombinant type I interferon to determine whether EcVig expression was induced. Poly(I:C) rapidly induced substantial expression of EcVig, whereas LPS stimulation did not appear to have any effect in grouper intestine. Expression levels of total EcVig and other IFN-stimulated genes (ISGs) were all significantly increased after RSIV and GIV infection. Furthermore, stimulation of recombinant type I IFN also increased EcVig expression. We conclude that EcVig may be a novel IFN-stimulated gene that demonstrates an antiviral immune response.

Original languageEnglish
Pages (from-to)415-422
Number of pages8
JournalFish and Shellfish Immunology
Volume58
DOIs
Publication statusPublished - 2016 Nov 1

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Epinephelus coioides
Interferon Type I
grouper
interferons
Viruses
immunoglobulins
Immunoglobulins
virus
Genes
Antiviral Agents
Lipopolysaccharides
RNA
gene
Poly I-C
genes
Double-Stranded RNA
lipopolysaccharides
immune response
Infectious spleen and kidney necrosis virus
Fish

All Science Journal Classification (ASJC) codes

  • Environmental Chemistry
  • Aquatic Science

Cite this

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title = "A member of the immunoglobulin superfamily, orange-spotted grouper novel immune gene EcVig, is induced by immune stimulants and type I interferon",
abstract = "A novel grouper immune gene, EcVig was identified in orange-spotted grouper (Epinephelus coioides). We recently determined that EcVig expression can be induced by infection with nervous necrosis virus (NNV, an RNA virus), whereas NNV replication may be suppressed when EcVig was overexpressed. Although EcVig appeared to be involved in grouper antiviral activity, its immune effects have not been well characterized. In the present study, two PAMPs (pathogen-associated molecular patterns; lipopolysaccharides [LPS] and synthetic double-stranded RNA polyriboinosinic-polyribocytidylic acid [poly(I:C)]), as well as fish DNA virus (red sea bream iridovirus, RSIV; grouper iridovirus, GIV), were used to study EcVig responses in orange-spotted grouper. In addition, groupers were given recombinant type I interferon to determine whether EcVig expression was induced. Poly(I:C) rapidly induced substantial expression of EcVig, whereas LPS stimulation did not appear to have any effect in grouper intestine. Expression levels of total EcVig and other IFN-stimulated genes (ISGs) were all significantly increased after RSIV and GIV infection. Furthermore, stimulation of recombinant type I IFN also increased EcVig expression. We conclude that EcVig may be a novel IFN-stimulated gene that demonstrates an antiviral immune response.",
author = "Yeh, {Ying Chun} and Wang, {Ting Yu} and Chou, {Hsin Yiu} and Lin, {Han You} and Chen, {Tzong Yueh} and Takashi Aoki and Wang, {Han Ching}",
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A member of the immunoglobulin superfamily, orange-spotted grouper novel immune gene EcVig, is induced by immune stimulants and type I interferon. / Yeh, Ying Chun; Wang, Ting Yu; Chou, Hsin Yiu; Lin, Han You; Chen, Tzong Yueh; Aoki, Takashi; Wang, Han Ching.

In: Fish and Shellfish Immunology, Vol. 58, 01.11.2016, p. 415-422.

Research output: Contribution to journalArticle

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AU - Yeh, Ying Chun

AU - Wang, Ting Yu

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AU - Chen, Tzong Yueh

AU - Aoki, Takashi

AU - Wang, Han Ching

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