A method to identify RNA A-to-I editing targets using I-specific cleavage and exon array analysis

Chao Neng Tseng, Hsueh Wei Chang, Joel Stocker, Hui Chun Wang, Chiu Chin Lu, Cheng Hsuan Wu, Jyuer Ger Yang, Chung Lung Cho, Hurng Wern Huang

Research output: Contribution to journalArticlepeer-review

6 Citations (Scopus)


RNA A-to-I editing is the most common single-base editing in the animal kingdom. Dysregulations of RNA A-to-I editing are associated with developmental defects in mouse and human diseases. Mouse knockout models deficient in ADAR activities show lethal phenotypes associated with defects in nervous system, failure of hematopoiesis and reduced tolerance to stress. While several methods of identifying RNA A-to-I editing sites are currently available, most of the critical editing targets responsible for the important biological functions of ADARs remain unknown. Here we report a method to systematically analyze RNA A-to-I editing targets by combining I-specific cleavage and exon array analysis. Our results show that I-specific cleavage on editing sites causes more than twofold signal reductions in edited exons of known targets such as Gria2, Htr2c, Gabra3 and Cyfip2 in mice. This method provides an experimental approach for genome-wide analysis of RNA A-to-I editing targets with exon-level resolution. We believe this method will help expedite inquiry into the roles of RNA A-to-I editing in various biological processes and diseases.

Original languageEnglish
Pages (from-to)38-45
Number of pages8
JournalMolecular and Cellular Probes
Issue number1
Publication statusPublished - 2013 Feb

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Cell Biology


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