A micro-volume viscosity measurement technique based on μPIV diffusometry

Yue Syun Sie, Han-Sheng Chuang

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Liquid viscosity is a vital metric in numerous biochemical applications. A precise bio-assay often needs to determine viscosity prior to processing. This article presents a simple viscosity measurement technique based on the μPIV diffusometry. Assessing Brownian motion of neutrally buoyant particles in a micro-sized chamber provides a fast link to the liquid viscosity. The ensemble cross-correlation algorithm was used to extract the viscosity information out of a series of particle images. The width of a correlation peak decreased with the increased viscosity. For an accurate measurement, the study also evaluated deviations resulting from hindered diffusion. A correction factor was obtained by comparing the measured viscosity of glycerol solutions with the reference data. A calibration curve was made to fix the shifted measurements. The advantages of this technique are as follows: small volume (<1 μL), noninvasiveness, ease of use, and low cost. The good agreement between the data measured from the μPIV system and a commercial vis-cometer validated the approach. To prove the effectiveness, the technique was used to study the viscosity change of dextran solutions and its effect on the kinematics of the micro-swimmer, Caenorhabditis ele-gans. A broad range of viscosity measurements (> 103 mPa s) were achieved. The demonstration confirms the possible use of the technique in other biological applications that requires broad-range and small-volume measurement capabilities for viscosity.

Original languageEnglish
Pages (from-to)65-72
Number of pages8
JournalMicrofluidics and Nanofluidics
Volume16
Issue number1-2
DOIs
Publication statusPublished - 2014 Jan 1

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Volume measurement
Viscosity measurement
particle image velocimetry
Viscosity
viscosity
Viscosity of liquids
Bioassay
Brownian movement
Glycerol
Demonstrations
bioassay
Calibration
liquids
glycerols
fixing
cross correlation
Processing
chambers
deviation
curves

All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Materials Chemistry

Cite this

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A micro-volume viscosity measurement technique based on μPIV diffusometry. / Sie, Yue Syun; Chuang, Han-Sheng.

In: Microfluidics and Nanofluidics, Vol. 16, No. 1-2, 01.01.2014, p. 65-72.

Research output: Contribution to journalArticle

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