Abstract
Liquid viscosity is a vital metric in numerous biochemical applications. A precise bio-assay often needs to determine viscosity prior to processing. This article presents a simple viscosity measurement technique based on the μPIV diffusometry. Assessing Brownian motion of neutrally buoyant particles in a micro-sized chamber provides a fast link to the liquid viscosity. The ensemble cross-correlation algorithm was used to extract the viscosity information out of a series of particle images. The width of a correlation peak decreased with the increased viscosity. For an accurate measurement, the study also evaluated deviations resulting from hindered diffusion. A correction factor was obtained by comparing the measured viscosity of glycerol solutions with the reference data. A calibration curve was made to fix the shifted measurements. The advantages of this technique are as follows: small volume (<1 μL), noninvasiveness, ease of use, and low cost. The good agreement between the data measured from the μPIV system and a commercial vis-cometer validated the approach. To prove the effectiveness, the technique was used to study the viscosity change of dextran solutions and its effect on the kinematics of the micro-swimmer, Caenorhabditis ele-gans. A broad range of viscosity measurements (> 103 mPa s) were achieved. The demonstration confirms the possible use of the technique in other biological applications that requires broad-range and small-volume measurement capabilities for viscosity.
| Original language | English |
|---|---|
| Pages (from-to) | 65-72 |
| Number of pages | 8 |
| Journal | Microfluidics and Nanofluidics |
| Volume | 16 |
| Issue number | 1-2 |
| DOIs | |
| Publication status | Published - 2014 Jan 1 |
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All Science Journal Classification (ASJC) codes
- Electronic, Optical and Magnetic Materials
- Condensed Matter Physics
- Materials Chemistry
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A micro-volume viscosity measurement technique based on μPIV diffusometry. / Sie, Yue Syun; Chuang, Han-Sheng.
In: Microfluidics and Nanofluidics, Vol. 16, No. 1-2, 01.01.2014, p. 65-72.Research output: Contribution to journal › Article
TY - JOUR
T1 - A micro-volume viscosity measurement technique based on μPIV diffusometry
AU - Sie, Yue Syun
AU - Chuang, Han-Sheng
PY - 2014/1/1
Y1 - 2014/1/1
N2 - Liquid viscosity is a vital metric in numerous biochemical applications. A precise bio-assay often needs to determine viscosity prior to processing. This article presents a simple viscosity measurement technique based on the μPIV diffusometry. Assessing Brownian motion of neutrally buoyant particles in a micro-sized chamber provides a fast link to the liquid viscosity. The ensemble cross-correlation algorithm was used to extract the viscosity information out of a series of particle images. The width of a correlation peak decreased with the increased viscosity. For an accurate measurement, the study also evaluated deviations resulting from hindered diffusion. A correction factor was obtained by comparing the measured viscosity of glycerol solutions with the reference data. A calibration curve was made to fix the shifted measurements. The advantages of this technique are as follows: small volume (<1 μL), noninvasiveness, ease of use, and low cost. The good agreement between the data measured from the μPIV system and a commercial vis-cometer validated the approach. To prove the effectiveness, the technique was used to study the viscosity change of dextran solutions and its effect on the kinematics of the micro-swimmer, Caenorhabditis ele-gans. A broad range of viscosity measurements (> 103 mPa s) were achieved. The demonstration confirms the possible use of the technique in other biological applications that requires broad-range and small-volume measurement capabilities for viscosity.
AB - Liquid viscosity is a vital metric in numerous biochemical applications. A precise bio-assay often needs to determine viscosity prior to processing. This article presents a simple viscosity measurement technique based on the μPIV diffusometry. Assessing Brownian motion of neutrally buoyant particles in a micro-sized chamber provides a fast link to the liquid viscosity. The ensemble cross-correlation algorithm was used to extract the viscosity information out of a series of particle images. The width of a correlation peak decreased with the increased viscosity. For an accurate measurement, the study also evaluated deviations resulting from hindered diffusion. A correction factor was obtained by comparing the measured viscosity of glycerol solutions with the reference data. A calibration curve was made to fix the shifted measurements. The advantages of this technique are as follows: small volume (<1 μL), noninvasiveness, ease of use, and low cost. The good agreement between the data measured from the μPIV system and a commercial vis-cometer validated the approach. To prove the effectiveness, the technique was used to study the viscosity change of dextran solutions and its effect on the kinematics of the micro-swimmer, Caenorhabditis ele-gans. A broad range of viscosity measurements (> 103 mPa s) were achieved. The demonstration confirms the possible use of the technique in other biological applications that requires broad-range and small-volume measurement capabilities for viscosity.
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UR - http://www.scopus.com/inward/citedby.url?scp=84899445894&partnerID=8YFLogxK
U2 - 10.1007/s10404-013-1219-4
DO - 10.1007/s10404-013-1219-4
M3 - Article
VL - 16
SP - 65
EP - 72
JO - Microfluidics and Nanofluidics
JF - Microfluidics and Nanofluidics
SN - 1613-4982
IS - 1-2
ER -