A microfluidic and potentiostatic sensor integrated with neopterin-imprinted poly(ethylene-co-vinyl alcohol) based electrode

Chen Hsin Hsieh, Hung Yin Lin, Chien Fu Lin, Hann Huei Tsai, Ying Zong Juang, Chun Yuen Huang, Bin-Da Liu, Mei Hwa Lee

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Neopterin is a catabolic product of guanosine triphosphate (GTP), a purine nucleotide. In human biological fluids such as urine, neopterin concentrations indicate the activation of cellular immune activation under the control of T helper cells. High neopterin concentrations in body fluids such as serum or urine also indicate the activation of cellular immunity, which is associated with the reaction to oxidative stress. In this work, neopterin is used as the target molecule and is then imprinted onto poly(ethylene-co-vinyl alcohol) via solvent evaporation. After removing the thin-film template molecules, the membrane is used as a sensing element for electrochemical urinalysis. Miniaturized devices were fabricated for continuous measurement of biomolecules in urine. A microfluidic channel made from polydimethylsiloxane (PDMS) is mounted on the electrochemical electrode chip. To maximize electrochemical response, the sensing area is a 30 × 1 mm2. In the random urine analysis, the cyclic voltammetry measurements of neopterin with additional recovery method achieved higher than 90% recovery gave the neopterin concentration 0.5 ng/mL.

Original languageEnglish
Title of host publicationNEMS 2011 - 6th IEEE International Conference on Nano/Micro Engineered and Molecular Systems
Pages575-578
Number of pages4
DOIs
Publication statusPublished - 2011 Oct 4
Event6th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2011 - Kaohsiung, Taiwan
Duration: 2011 Feb 202011 Feb 23

Publication series

NameNEMS 2011 - 6th IEEE International Conference on Nano/Micro Engineered and Molecular Systems

Other

Other6th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2011
CountryTaiwan
CityKaohsiung
Period11-02-2011-02-23

All Science Journal Classification (ASJC) codes

  • Atomic and Molecular Physics, and Optics

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    Hsieh, C. H., Lin, H. Y., Lin, C. F., Tsai, H. H., Juang, Y. Z., Huang, C. Y., Liu, B-D., & Lee, M. H. (2011). A microfluidic and potentiostatic sensor integrated with neopterin-imprinted poly(ethylene-co-vinyl alcohol) based electrode. In NEMS 2011 - 6th IEEE International Conference on Nano/Micro Engineered and Molecular Systems (pp. 575-578). [6017421] (NEMS 2011 - 6th IEEE International Conference on Nano/Micro Engineered and Molecular Systems). https://doi.org/10.1109/NEMS.2011.6017421