TY - GEN
T1 - A microfluidic and potentiostatic sensor integrated with neopterin-imprinted poly(ethylene-co-vinyl alcohol) based electrode
AU - Hsieh, Chen Hsin
AU - Lin, Hung Yin
AU - Lin, Chien Fu
AU - Tsai, Hann Huei
AU - Juang, Ying Zong
AU - Huang, Chun Yuen
AU - Liu, Bin Da
AU - Lee, Mei Hwa
PY - 2011
Y1 - 2011
N2 - Neopterin is a catabolic product of guanosine triphosphate (GTP), a purine nucleotide. In human biological fluids such as urine, neopterin concentrations indicate the activation of cellular immune activation under the control of T helper cells. High neopterin concentrations in body fluids such as serum or urine also indicate the activation of cellular immunity, which is associated with the reaction to oxidative stress. In this work, neopterin is used as the target molecule and is then imprinted onto poly(ethylene-co-vinyl alcohol) via solvent evaporation. After removing the thin-film template molecules, the membrane is used as a sensing element for electrochemical urinalysis. Miniaturized devices were fabricated for continuous measurement of biomolecules in urine. A microfluidic channel made from polydimethylsiloxane (PDMS) is mounted on the electrochemical electrode chip. To maximize electrochemical response, the sensing area is a 30 × 1 mm2. In the random urine analysis, the cyclic voltammetry measurements of neopterin with additional recovery method achieved higher than 90% recovery gave the neopterin concentration 0.5 ng/mL.
AB - Neopterin is a catabolic product of guanosine triphosphate (GTP), a purine nucleotide. In human biological fluids such as urine, neopterin concentrations indicate the activation of cellular immune activation under the control of T helper cells. High neopterin concentrations in body fluids such as serum or urine also indicate the activation of cellular immunity, which is associated with the reaction to oxidative stress. In this work, neopterin is used as the target molecule and is then imprinted onto poly(ethylene-co-vinyl alcohol) via solvent evaporation. After removing the thin-film template molecules, the membrane is used as a sensing element for electrochemical urinalysis. Miniaturized devices were fabricated for continuous measurement of biomolecules in urine. A microfluidic channel made from polydimethylsiloxane (PDMS) is mounted on the electrochemical electrode chip. To maximize electrochemical response, the sensing area is a 30 × 1 mm2. In the random urine analysis, the cyclic voltammetry measurements of neopterin with additional recovery method achieved higher than 90% recovery gave the neopterin concentration 0.5 ng/mL.
UR - http://www.scopus.com/inward/record.url?scp=80053329521&partnerID=8YFLogxK
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U2 - 10.1109/NEMS.2011.6017453
DO - 10.1109/NEMS.2011.6017453
M3 - Conference contribution
AN - SCOPUS:80053329521
SN - 9781612847757
T3 - NEMS 2011 - 6th IEEE International Conference on Nano/Micro Engineered and Molecular Systems
SP - 711
EP - 714
BT - NEMS 2011 - 6th IEEE International Conference on Nano/Micro Engineered and Molecular Systems
T2 - 6th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2011
Y2 - 20 February 2011 through 23 February 2011
ER -