A microfluidic-based cell culture platform for cellular and subcellular imaging

Dar-Bin Shieh, Chia Chun Hsieh, Song Bin Huang, Gwo Bin Lee

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Citation (Scopus)

Abstract

This study reports a new microfluidic-based cell culture platform. Moreover, microheaters, a micro temperature sensor, micropumps were integrated into the system to achieve a self-contained device for standard perfusion mammalian cell culture. One of key features of the proposed platform is the design of ultra-thin cell culture chamber allowing real-time cellular and sub-cellular imaging. The entire cell culture process can be performed automatically without human intervention. The developed platform was used for study of drug delivery on oral cancer cells. Experimental data showed that it can successfully monitor the dividing process of cancer cells. Besides, the cell response to anti-cancer drugs was also investigated. The developed platform can be promising for cell-based study.

Original languageEnglish
Title of host publication4th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2009
Pages223-226
Number of pages4
DOIs
Publication statusPublished - 2009 Oct 12
Event4th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2009 - Shenzhen, China
Duration: 2009 Jan 52009 Jan 8

Publication series

Name4th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2009

Other

Other4th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2009
CountryChina
CityShenzhen
Period09-01-0509-01-08

Fingerprint

Cell culture
Microfluidics
Imaging techniques
Cells
Temperature sensors
Drug delivery

All Science Journal Classification (ASJC) codes

  • Electrical and Electronic Engineering

Cite this

Shieh, D-B., Hsieh, C. C., Huang, S. B., & Lee, G. B. (2009). A microfluidic-based cell culture platform for cellular and subcellular imaging. In 4th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2009 (pp. 223-226). [5068564] (4th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2009). https://doi.org/10.1109/NEMS.2009.5068564
Shieh, Dar-Bin ; Hsieh, Chia Chun ; Huang, Song Bin ; Lee, Gwo Bin. / A microfluidic-based cell culture platform for cellular and subcellular imaging. 4th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2009. 2009. pp. 223-226 (4th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2009).
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Shieh, D-B, Hsieh, CC, Huang, SB & Lee, GB 2009, A microfluidic-based cell culture platform for cellular and subcellular imaging. in 4th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2009., 5068564, 4th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2009, pp. 223-226, 4th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2009, Shenzhen, China, 09-01-05. https://doi.org/10.1109/NEMS.2009.5068564

A microfluidic-based cell culture platform for cellular and subcellular imaging. / Shieh, Dar-Bin; Hsieh, Chia Chun; Huang, Song Bin; Lee, Gwo Bin.

4th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2009. 2009. p. 223-226 5068564 (4th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2009).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

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Shieh D-B, Hsieh CC, Huang SB, Lee GB. A microfluidic-based cell culture platform for cellular and subcellular imaging. In 4th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2009. 2009. p. 223-226. 5068564. (4th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2009). https://doi.org/10.1109/NEMS.2009.5068564