A microfluidic device for capture of single cells and impedance measurement

Min Haw Wang, Min Feng Kao, Haw Juin Liu, Wai Hong Kan, Yi Chu Hsu, Ling-Sheng Jang

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

A microfluidic device for capture of single cells and impedance measurement is presented. The device consists of a PDMS channel with three micro pillars and a glass substrate with electrodes. The experiments demonstrated that the HeLa cell (human cervical epithelioid carcinoma) was successfully captured by the micro pillars and its impedance was measured by impedance spectroscopy. The range of operation voltage is from 0.1 V to 1.5 V and the scan frequency is from 1 kHz to 100 kHz. According to experimental results, the HeLa cell is capacitive and its electrical model can be simplified to the parallel connection with one resistor and one capacitor. This developed technique for cell impedance analysis possesses advantages of physical capture, low cost, and easy of fabrication and measurement.

Original languageEnglish
Title of host publicationProceedings of the 2nd IEEE International Conference on Nano/Micro Engineered and Molecular Systems, IEEE NEMS 2007
Pages711-714
Number of pages4
DOIs
Publication statusPublished - 2007
Event2007 2nd IEEE International Conference on Nano/Micro Engineered and Molecular Systems, IEEE NEMS 2007 - Bangkok, Thailand
Duration: 2007 Jan 162007 Jan 19

Other

Other2007 2nd IEEE International Conference on Nano/Micro Engineered and Molecular Systems, IEEE NEMS 2007
CountryThailand
CityBangkok
Period07-01-1607-01-19

Fingerprint

Microfluidics
Resistors
Capacitors
Spectroscopy
Fabrication
Glass
Electrodes
Electric potential
Substrates
Costs
Experiments

All Science Journal Classification (ASJC) codes

  • Computer Networks and Communications
  • Computer Science Applications
  • Electrical and Electronic Engineering

Cite this

Wang, M. H., Kao, M. F., Liu, H. J., Kan, W. H., Hsu, Y. C., & Jang, L-S. (2007). A microfluidic device for capture of single cells and impedance measurement. In Proceedings of the 2nd IEEE International Conference on Nano/Micro Engineered and Molecular Systems, IEEE NEMS 2007 (pp. 711-714). [4160420] https://doi.org/10.1109/NEMS.2007.352117
Wang, Min Haw ; Kao, Min Feng ; Liu, Haw Juin ; Kan, Wai Hong ; Hsu, Yi Chu ; Jang, Ling-Sheng. / A microfluidic device for capture of single cells and impedance measurement. Proceedings of the 2nd IEEE International Conference on Nano/Micro Engineered and Molecular Systems, IEEE NEMS 2007. 2007. pp. 711-714
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abstract = "A microfluidic device for capture of single cells and impedance measurement is presented. The device consists of a PDMS channel with three micro pillars and a glass substrate with electrodes. The experiments demonstrated that the HeLa cell (human cervical epithelioid carcinoma) was successfully captured by the micro pillars and its impedance was measured by impedance spectroscopy. The range of operation voltage is from 0.1 V to 1.5 V and the scan frequency is from 1 kHz to 100 kHz. According to experimental results, the HeLa cell is capacitive and its electrical model can be simplified to the parallel connection with one resistor and one capacitor. This developed technique for cell impedance analysis possesses advantages of physical capture, low cost, and easy of fabrication and measurement.",
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Wang, MH, Kao, MF, Liu, HJ, Kan, WH, Hsu, YC & Jang, L-S 2007, A microfluidic device for capture of single cells and impedance measurement. in Proceedings of the 2nd IEEE International Conference on Nano/Micro Engineered and Molecular Systems, IEEE NEMS 2007., 4160420, pp. 711-714, 2007 2nd IEEE International Conference on Nano/Micro Engineered and Molecular Systems, IEEE NEMS 2007, Bangkok, Thailand, 07-01-16. https://doi.org/10.1109/NEMS.2007.352117

A microfluidic device for capture of single cells and impedance measurement. / Wang, Min Haw; Kao, Min Feng; Liu, Haw Juin; Kan, Wai Hong; Hsu, Yi Chu; Jang, Ling-Sheng.

Proceedings of the 2nd IEEE International Conference on Nano/Micro Engineered and Molecular Systems, IEEE NEMS 2007. 2007. p. 711-714 4160420.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

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Wang MH, Kao MF, Liu HJ, Kan WH, Hsu YC, Jang L-S. A microfluidic device for capture of single cells and impedance measurement. In Proceedings of the 2nd IEEE International Conference on Nano/Micro Engineered and Molecular Systems, IEEE NEMS 2007. 2007. p. 711-714. 4160420 https://doi.org/10.1109/NEMS.2007.352117