TY - GEN
T1 - A microfluidic device for capture of single cells and impedance measurement
AU - Wang, Min Haw
AU - Kao, Min Feng
AU - Liu, Haw Juin
AU - Kan, Wai Hong
AU - Hsu, Yi Chu
AU - Jang, Ling Sheng
PY - 2007/8/28
Y1 - 2007/8/28
N2 - A microfluidic device for capture of single cells and impedance measurement is presented. The device consists of a PDMS channel with three micro pillars and a glass substrate with electrodes. The experiments demonstrated that the HeLa cell (human cervical epithelioid carcinoma) was successfully captured by the micro pillars and its impedance was measured by impedance spectroscopy. The range of operation voltage is from 0.1 V to 1.5 V and the scan frequency is from 1 kHz to 100 kHz. According to experimental results, the HeLa cell is capacitive and its electrical model can be simplified to the parallel connection with one resistor and one capacitor. This developed technique for cell impedance analysis possesses advantages of physical capture, low cost, and easy of fabrication and measurement.
AB - A microfluidic device for capture of single cells and impedance measurement is presented. The device consists of a PDMS channel with three micro pillars and a glass substrate with electrodes. The experiments demonstrated that the HeLa cell (human cervical epithelioid carcinoma) was successfully captured by the micro pillars and its impedance was measured by impedance spectroscopy. The range of operation voltage is from 0.1 V to 1.5 V and the scan frequency is from 1 kHz to 100 kHz. According to experimental results, the HeLa cell is capacitive and its electrical model can be simplified to the parallel connection with one resistor and one capacitor. This developed technique for cell impedance analysis possesses advantages of physical capture, low cost, and easy of fabrication and measurement.
UR - http://www.scopus.com/inward/record.url?scp=34548129963&partnerID=8YFLogxK
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U2 - 10.1109/NEMS.2007.352117
DO - 10.1109/NEMS.2007.352117
M3 - Conference contribution
AN - SCOPUS:34548129963
SN - 1424406102
SN - 9781424406104
T3 - Proceedings of the 2nd IEEE International Conference on Nano/Micro Engineered and Molecular Systems, IEEE NEMS 2007
SP - 711
EP - 714
BT - Proceedings of the 2nd IEEE International Conference on Nano/Micro Engineered and Molecular Systems, IEEE NEMS 2007
T2 - 2007 2nd IEEE International Conference on Nano/Micro Engineered and Molecular Systems, IEEE NEMS 2007
Y2 - 16 January 2007 through 19 January 2007
ER -