TY - GEN
T1 - A microfluidic mammalian cell sorter with thermal gelation polymer solution
AU - Shirasaki, Y.
AU - Goto, M.
AU - Sugino, H.
AU - Arakawa, T.
AU - Yoon, D.
AU - Mizuno, J.
AU - Shoji, S.
AU - Funatsu, T.
AU - Ohara, O.
PY - 2010
Y1 - 2010
N2 - We report a development of an on-chip sorting system for fluorescently labeled mammalian cells based on a reversible sol-gel transition of thermoreversible gelation polymer controlled by local heating with infrared laser. The new system allowed to utilize the energy from the infrared laser irradiation efficiently to achieve an extensive gelation of the polymer in the channel. This enabled the new system to sort mammalian cells with 97% viability in the large channel with 50-100 μm in depth and width. Moreover, we succeeded in sorting with 100% recovery and over 99% purity by introducing a 3D flow focusing.
AB - We report a development of an on-chip sorting system for fluorescently labeled mammalian cells based on a reversible sol-gel transition of thermoreversible gelation polymer controlled by local heating with infrared laser. The new system allowed to utilize the energy from the infrared laser irradiation efficiently to achieve an extensive gelation of the polymer in the channel. This enabled the new system to sort mammalian cells with 97% viability in the large channel with 50-100 μm in depth and width. Moreover, we succeeded in sorting with 100% recovery and over 99% purity by introducing a 3D flow focusing.
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M3 - Conference contribution
AN - SCOPUS:84883796238
SN - 9781618390622
T3 - 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010
SP - 1571
EP - 1573
BT - 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010
T2 - 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010
Y2 - 3 October 2010 through 7 October 2010
ER -