TY - GEN
T1 - A microfluidic platform for diagnosis of ovarian clear cell carcinoma via quantification of fxyd2 gene
AU - Liu, Ting Hang
AU - Lin, Chang Ni
AU - Hsu, Keng Fu
AU - Lee, Gwo Bin
N1 - Funding Information:
The authors would like to thank 1) Ministry of Science and Technology (MOST) of Taiwan for funding this work (MOST 107-2314-B-007-005), 2) National Health Research Institutes (NHRI-EX108-10828EI), and 3) Ministry of Education (Taiwan) (“Higher Education Sprout Project” under a grant number of 108Q2713E1).
Funding Information:
The work was financially supported by a grant from La Région Auvergne-Rhône-Alpes.
Publisher Copyright:
© 2019 CBMS-0001.
PY - 2019
Y1 - 2019
N2 - Ovarian cancer (OvCa), with an extremely low 5-year survival rate, is difficult for early diagnosis and prognosis monitoring due to poorly sensitive and specific cancer biomarkers for detection. In this work, an integrated microfluidic system capable of isolation of OvCa cells from liquid biopsy (such as ascites) via aptamer coated-magnetic beads and RT-qPCR for quantification of the FXYD2 expression level was developed for automatic diagnosis of ovarian clear cell carcinoma. Experimental results showed that a limit of detection could be as low as 10 cells/reaction, which is satisfactory for future clinical trials.
AB - Ovarian cancer (OvCa), with an extremely low 5-year survival rate, is difficult for early diagnosis and prognosis monitoring due to poorly sensitive and specific cancer biomarkers for detection. In this work, an integrated microfluidic system capable of isolation of OvCa cells from liquid biopsy (such as ascites) via aptamer coated-magnetic beads and RT-qPCR for quantification of the FXYD2 expression level was developed for automatic diagnosis of ovarian clear cell carcinoma. Experimental results showed that a limit of detection could be as low as 10 cells/reaction, which is satisfactory for future clinical trials.
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M3 - Conference contribution
AN - SCOPUS:85094982118
T3 - 23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019
SP - 622
EP - 623
BT - 23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019
PB - Chemical and Biological Microsystems Society
T2 - 23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019
Y2 - 27 October 2019 through 31 October 2019
ER -