A microfluidic platform for diagnosis of ovarian clear cell carcinoma via quantification of fxyd2 gene

Ting Hang Liu, Chang Ni Lin, Keng Fu Hsu, Gwo Bin Lee

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Ovarian cancer (OvCa), with an extremely low 5-year survival rate, is difficult for early diagnosis and prognosis monitoring due to poorly sensitive and specific cancer biomarkers for detection. In this work, an integrated microfluidic system capable of isolation of OvCa cells from liquid biopsy (such as ascites) via aptamer coated-magnetic beads and RT-qPCR for quantification of the FXYD2 expression level was developed for automatic diagnosis of ovarian clear cell carcinoma. Experimental results showed that a limit of detection could be as low as 10 cells/reaction, which is satisfactory for future clinical trials.

Original languageEnglish
Title of host publication23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019
PublisherChemical and Biological Microsystems Society
Pages622-623
Number of pages2
ISBN (Electronic)9781733419000
Publication statusPublished - 2019
Event23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019 - Basel, Switzerland
Duration: 2019 Oct 272019 Oct 31

Publication series

Name23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019

Conference

Conference23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019
CountrySwitzerland
CityBasel
Period19-10-2719-10-31

All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Chemical Engineering (miscellaneous)

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