TY - GEN
T1 - A microfluidic system for detection of cholangiocarcinoma cells by using heparan sulfate octasaccharides
AU - Tsai, Wei Chun
AU - Hung, Lien Yu
AU - Huang, Teng Yi
AU - Shan, Yen Shen
AU - Hung, Shang Cheng
AU - Lee, Gwo Bin
N1 - Funding Information:
ACKNOWLEDGMENTS The authors would like to gratefully thank Ministry of Science and Technology in Taiwan (105-2119-M-007-009) and the ‘‘Towards A World-class University’’ Project for the financial support to this study. The authors also appreciate the assistance of experiment by all members in the Microfluidic Biochip Lab, Department of Power Mechanical Engineering, National Tsing Hua University, Taiwan.
Publisher Copyright:
© 2017 IEEE.
PY - 2017/8/25
Y1 - 2017/8/25
N2 - This study reports a magnetic-bead based microfluidic system to capture cholangiocarcinoma cells by using structurally well-defined heparan sulfate (HS) octasaccharides. Ten synthetic HS octasaccharides (from SCH-43 to SCH-52) were coated on magnetic beads for this study, respectively. An integrated microfluidic system equipped with micromixers, normally-closed valves, and microchambers was developed such that the entire cancer cell isolation process could be automated. The experimental results have revealed that SCH-45 and SCH-46 of 100 μM-coated magnetic beads were capable to capture more than 70% of Huh28 cells, which is higher than the one by using epithelial cell adhesion molecule (EpCAM)-coated beads. Furthermore, they also exhibited high specificity toward target cells. It is the first time that HS octasaccharides were demonstrated as an excellent affinity reagent for cancer cell isolation.
AB - This study reports a magnetic-bead based microfluidic system to capture cholangiocarcinoma cells by using structurally well-defined heparan sulfate (HS) octasaccharides. Ten synthetic HS octasaccharides (from SCH-43 to SCH-52) were coated on magnetic beads for this study, respectively. An integrated microfluidic system equipped with micromixers, normally-closed valves, and microchambers was developed such that the entire cancer cell isolation process could be automated. The experimental results have revealed that SCH-45 and SCH-46 of 100 μM-coated magnetic beads were capable to capture more than 70% of Huh28 cells, which is higher than the one by using epithelial cell adhesion molecule (EpCAM)-coated beads. Furthermore, they also exhibited high specificity toward target cells. It is the first time that HS octasaccharides were demonstrated as an excellent affinity reagent for cancer cell isolation.
UR - http://www.scopus.com/inward/record.url?scp=85030859171&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85030859171&partnerID=8YFLogxK
U2 - 10.1109/NEMS.2017.8017070
DO - 10.1109/NEMS.2017.8017070
M3 - Conference contribution
AN - SCOPUS:85030859171
T3 - 2017 IEEE 12th International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2017
SP - 489
EP - 493
BT - 2017 IEEE 12th International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2017
PB - Institute of Electrical and Electronics Engineers Inc.
T2 - 12th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2017
Y2 - 9 April 2017 through 12 April 2017
ER -
