A microfluidic system for detection of cholangiocarcinoma cells by using heparan sulfate octasaccharides

Wei Chun Tsai, Lien Yu Hung, Teng Yi Huang, Yan-Shen Shan, Shang Cheng Hung, Gwo Bin Lee

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

This study reports a magnetic-bead based microfluidic system to capture cholangiocarcinoma cells by using structurally well-defined heparan sulfate (HS) octasaccharides. Ten synthetic HS octasaccharides (from SCH-43 to SCH-52) were coated on magnetic beads for this study, respectively. An integrated microfluidic system equipped with micromixers, normally-closed valves, and microchambers was developed such that the entire cancer cell isolation process could be automated. The experimental results have revealed that SCH-45 and SCH-46 of 100 μM-coated magnetic beads were capable to capture more than 70% of Huh28 cells, which is higher than the one by using epithelial cell adhesion molecule (EpCAM)-coated beads. Furthermore, they also exhibited high specificity toward target cells. It is the first time that HS octasaccharides were demonstrated as an excellent affinity reagent for cancer cell isolation.

Original languageEnglish
Title of host publication2017 IEEE 12th International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2017
PublisherInstitute of Electrical and Electronics Engineers Inc.
Pages489-493
Number of pages5
ISBN (Electronic)9781509030590
DOIs
Publication statusPublished - 2017 Aug 25
Event12th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2017 - Los Angeles, United States
Duration: 2017 Apr 92017 Apr 12

Publication series

Name2017 IEEE 12th International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2017

Other

Other12th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2017
CountryUnited States
CityLos Angeles
Period17-04-0917-04-12

Fingerprint

Heparitin Sulfate
Microfluidics
beads
sulfates
cells
Cells
isolation
cancer
Cell adhesion
Cell Adhesion Molecules
reagents
affinity
Molecules
adhesion
Sulfates
molecules

All Science Journal Classification (ASJC) codes

  • Electrical and Electronic Engineering
  • Mechanical Engineering
  • Electronic, Optical and Magnetic Materials
  • Instrumentation

Cite this

Tsai, W. C., Hung, L. Y., Huang, T. Y., Shan, Y-S., Hung, S. C., & Lee, G. B. (2017). A microfluidic system for detection of cholangiocarcinoma cells by using heparan sulfate octasaccharides. In 2017 IEEE 12th International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2017 (pp. 489-493). [8017070] (2017 IEEE 12th International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2017). Institute of Electrical and Electronics Engineers Inc.. https://doi.org/10.1109/NEMS.2017.8017070
Tsai, Wei Chun ; Hung, Lien Yu ; Huang, Teng Yi ; Shan, Yan-Shen ; Hung, Shang Cheng ; Lee, Gwo Bin. / A microfluidic system for detection of cholangiocarcinoma cells by using heparan sulfate octasaccharides. 2017 IEEE 12th International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2017. Institute of Electrical and Electronics Engineers Inc., 2017. pp. 489-493 (2017 IEEE 12th International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2017).
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title = "A microfluidic system for detection of cholangiocarcinoma cells by using heparan sulfate octasaccharides",
abstract = "This study reports a magnetic-bead based microfluidic system to capture cholangiocarcinoma cells by using structurally well-defined heparan sulfate (HS) octasaccharides. Ten synthetic HS octasaccharides (from SCH-43 to SCH-52) were coated on magnetic beads for this study, respectively. An integrated microfluidic system equipped with micromixers, normally-closed valves, and microchambers was developed such that the entire cancer cell isolation process could be automated. The experimental results have revealed that SCH-45 and SCH-46 of 100 μM-coated magnetic beads were capable to capture more than 70{\%} of Huh28 cells, which is higher than the one by using epithelial cell adhesion molecule (EpCAM)-coated beads. Furthermore, they also exhibited high specificity toward target cells. It is the first time that HS octasaccharides were demonstrated as an excellent affinity reagent for cancer cell isolation.",
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Tsai, WC, Hung, LY, Huang, TY, Shan, Y-S, Hung, SC & Lee, GB 2017, A microfluidic system for detection of cholangiocarcinoma cells by using heparan sulfate octasaccharides. in 2017 IEEE 12th International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2017., 8017070, 2017 IEEE 12th International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2017, Institute of Electrical and Electronics Engineers Inc., pp. 489-493, 12th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2017, Los Angeles, United States, 17-04-09. https://doi.org/10.1109/NEMS.2017.8017070

A microfluidic system for detection of cholangiocarcinoma cells by using heparan sulfate octasaccharides. / Tsai, Wei Chun; Hung, Lien Yu; Huang, Teng Yi; Shan, Yan-Shen; Hung, Shang Cheng; Lee, Gwo Bin.

2017 IEEE 12th International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2017. Institute of Electrical and Electronics Engineers Inc., 2017. p. 489-493 8017070 (2017 IEEE 12th International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2017).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

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Tsai WC, Hung LY, Huang TY, Shan Y-S, Hung SC, Lee GB. A microfluidic system for detection of cholangiocarcinoma cells by using heparan sulfate octasaccharides. In 2017 IEEE 12th International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2017. Institute of Electrical and Electronics Engineers Inc. 2017. p. 489-493. 8017070. (2017 IEEE 12th International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2017). https://doi.org/10.1109/NEMS.2017.8017070