A microfluidic system for detection of cholangiocarcinoma cells by using heparan sulfate octasaccharides

Wei Chun Tsai; Lien Yu Hung; Teng Yi Huang; Yen Shen Shan; Shang Cheng Hung; Gwo Bin Lee

doi:10.1109/NEMS.2017.8017070

A microfluidic system for detection of cholangiocarcinoma cells by using heparan sulfate octasaccharides

Wei Chun Tsai, Lien Yu Hung, Teng Yi Huang, Yen Shen Shan, Shang Cheng Hung, Gwo Bin Lee

Institute of Clinical Medicine

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

4 Citations (Scopus)

Abstract

This study reports a magnetic-bead based microfluidic system to capture cholangiocarcinoma cells by using structurally well-defined heparan sulfate (HS) octasaccharides. Ten synthetic HS octasaccharides (from SCH-43 to SCH-52) were coated on magnetic beads for this study, respectively. An integrated microfluidic system equipped with micromixers, normally-closed valves, and microchambers was developed such that the entire cancer cell isolation process could be automated. The experimental results have revealed that SCH-45 and SCH-46 of 100 μM-coated magnetic beads were capable to capture more than 70% of Huh28 cells, which is higher than the one by using epithelial cell adhesion molecule (EpCAM)-coated beads. Furthermore, they also exhibited high specificity toward target cells. It is the first time that HS octasaccharides were demonstrated as an excellent affinity reagent for cancer cell isolation.

Original language	English
Title of host publication	2017 IEEE 12th International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2017
Publisher	Institute of Electrical and Electronics Engineers Inc.
Pages	489-493
Number of pages	5
ISBN (Electronic)	9781509030590
DOIs	https://doi.org/10.1109/NEMS.2017.8017070
Publication status	Published - 2017 Aug 25
Event	12th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2017 - Los Angeles, United States Duration: 2017 Apr 9 → 2017 Apr 12

Publication series

Name	2017 IEEE 12th International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2017

Other

Other	12th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2017
Country/Territory	United States
City	Los Angeles
Period	17-04-09 → 17-04-12

All Science Journal Classification (ASJC) codes

Electrical and Electronic Engineering
Mechanical Engineering
Electronic, Optical and Magnetic Materials
Instrumentation

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1109/NEMS.2017.8017070

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Tsai, W. C., Hung, L. Y., Huang, T. Y., Shan, Y. S., Hung, S. C., & Lee, G. B. (2017). A microfluidic system for detection of cholangiocarcinoma cells by using heparan sulfate octasaccharides. In 2017 IEEE 12th International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2017 (pp. 489-493). Article 8017070 (2017 IEEE 12th International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2017). Institute of Electrical and Electronics Engineers Inc.. https://doi.org/10.1109/NEMS.2017.8017070

Tsai, Wei Chun ; Hung, Lien Yu ; Huang, Teng Yi et al. / A microfluidic system for detection of cholangiocarcinoma cells by using heparan sulfate octasaccharides. 2017 IEEE 12th International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2017. Institute of Electrical and Electronics Engineers Inc., 2017. pp. 489-493 (2017 IEEE 12th International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2017).

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title = "A microfluidic system for detection of cholangiocarcinoma cells by using heparan sulfate octasaccharides",

abstract = "This study reports a magnetic-bead based microfluidic system to capture cholangiocarcinoma cells by using structurally well-defined heparan sulfate (HS) octasaccharides. Ten synthetic HS octasaccharides (from SCH-43 to SCH-52) were coated on magnetic beads for this study, respectively. An integrated microfluidic system equipped with micromixers, normally-closed valves, and microchambers was developed such that the entire cancer cell isolation process could be automated. The experimental results have revealed that SCH-45 and SCH-46 of 100 μM-coated magnetic beads were capable to capture more than 70% of Huh28 cells, which is higher than the one by using epithelial cell adhesion molecule (EpCAM)-coated beads. Furthermore, they also exhibited high specificity toward target cells. It is the first time that HS octasaccharides were demonstrated as an excellent affinity reagent for cancer cell isolation.",

author = "Tsai, {Wei Chun} and Hung, {Lien Yu} and Huang, {Teng Yi} and Shan, {Yen Shen} and Hung, {Shang Cheng} and Lee, {Gwo Bin}",

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doi = "10.1109/NEMS.2017.8017070",

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series = "2017 IEEE 12th International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2017",

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Tsai, WC, Hung, LY, Huang, TY, Shan, YS, Hung, SC & Lee, GB 2017, A microfluidic system for detection of cholangiocarcinoma cells by using heparan sulfate octasaccharides. in 2017 IEEE 12th International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2017., 8017070, 2017 IEEE 12th International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2017, Institute of Electrical and Electronics Engineers Inc., pp. 489-493, 12th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2017, Los Angeles, United States, 17-04-09. https://doi.org/10.1109/NEMS.2017.8017070

A microfluidic system for detection of cholangiocarcinoma cells by using heparan sulfate octasaccharides. / Tsai, Wei Chun; Hung, Lien Yu; Huang, Teng Yi et al.
2017 IEEE 12th International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2017. Institute of Electrical and Electronics Engineers Inc., 2017. p. 489-493 8017070 (2017 IEEE 12th International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2017).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

TY - GEN

T1 - A microfluidic system for detection of cholangiocarcinoma cells by using heparan sulfate octasaccharides

AU - Tsai, Wei Chun

AU - Hung, Lien Yu

AU - Huang, Teng Yi

AU - Shan, Yen Shen

AU - Hung, Shang Cheng

AU - Lee, Gwo Bin

PY - 2017/8/25

Y1 - 2017/8/25

N2 - This study reports a magnetic-bead based microfluidic system to capture cholangiocarcinoma cells by using structurally well-defined heparan sulfate (HS) octasaccharides. Ten synthetic HS octasaccharides (from SCH-43 to SCH-52) were coated on magnetic beads for this study, respectively. An integrated microfluidic system equipped with micromixers, normally-closed valves, and microchambers was developed such that the entire cancer cell isolation process could be automated. The experimental results have revealed that SCH-45 and SCH-46 of 100 μM-coated magnetic beads were capable to capture more than 70% of Huh28 cells, which is higher than the one by using epithelial cell adhesion molecule (EpCAM)-coated beads. Furthermore, they also exhibited high specificity toward target cells. It is the first time that HS octasaccharides were demonstrated as an excellent affinity reagent for cancer cell isolation.

AB - This study reports a magnetic-bead based microfluidic system to capture cholangiocarcinoma cells by using structurally well-defined heparan sulfate (HS) octasaccharides. Ten synthetic HS octasaccharides (from SCH-43 to SCH-52) were coated on magnetic beads for this study, respectively. An integrated microfluidic system equipped with micromixers, normally-closed valves, and microchambers was developed such that the entire cancer cell isolation process could be automated. The experimental results have revealed that SCH-45 and SCH-46 of 100 μM-coated magnetic beads were capable to capture more than 70% of Huh28 cells, which is higher than the one by using epithelial cell adhesion molecule (EpCAM)-coated beads. Furthermore, they also exhibited high specificity toward target cells. It is the first time that HS octasaccharides were demonstrated as an excellent affinity reagent for cancer cell isolation.

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BT - 2017 IEEE 12th International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2017

PB - Institute of Electrical and Electronics Engineers Inc.

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Y2 - 9 April 2017 through 12 April 2017

ER -

Tsai WC, Hung LY, Huang TY, Shan YS, Hung SC, Lee GB. A microfluidic system for detection of cholangiocarcinoma cells by using heparan sulfate octasaccharides. In 2017 IEEE 12th International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2017. Institute of Electrical and Electronics Engineers Inc. 2017. p. 489-493. 8017070. (2017 IEEE 12th International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2017). doi: 10.1109/NEMS.2017.8017070

A microfluidic system for detection of cholangiocarcinoma cells by using heparan sulfate octasaccharides

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