A microfluidic system integrated with optical detection devices for automatic detection of C-reactive protein

Wen Bin Lee; Yin He Chen; Hsin I. Lin; Chao June Huang; Shu Chu Shiesh; Gwo Bin Lee

doi:10.1109/MEMSYS.2010.5442372

A microfluidic system integrated with optical detection devices for automatic detection of C-reactive protein

Wen Bin Lee, Yin He Chen, Hsin I. Lin, Chao June Huang, Shu Chu Shiesh, Gwo Bin Lee

Department of Medical Laboratory Science and Biotechnology

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

2 Citations (Scopus)

Abstract

C-reactive protein (CRP) is commonly known as a clinical marker, which has been identified as an independent predictor for cardiovascular diseases such as heart attack and stroke. In this study, a new microfluidic system for automatic measurement of CRP is reported. The entire process including sample pre-treatment, incubation between target CRP and anti-CRP antibody, developing process and detection can be automatically performed on a single chip. In addition, chemiluminescent signal was measured by using a home-made luminometer to quantify the concentration of CRP. When compared to previous works, not only can the new chip perform the entire process by integrating a new micro-injector, but a new CRP-specific DNA aptamer was also used for CRP measurement. Experimental data showed that the developed system can automate the entire process within 30 minutes with a detection limit of 0.0125 mgL^-1, which was superior to previous works.

Original language	English
Title of host publication	MEMS 2010 - The 23rd IEEE International Conference on Micro Electro Mechanical Systems, Technical Digest
Pages	979-982
Number of pages	4
DOIs	https://doi.org/10.1109/MEMSYS.2010.5442372
Publication status	Published - 2010 Jun 1
Event	23rd IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2010 - Hong Kong, China Duration: 2010 Jan 24 → 2010 Jan 28

Publication series

Name	Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)
ISSN (Print)	1084-6999

Other

Other	23rd IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2010
Country/Territory	China
City	Hong Kong
Period	10-01-24 → 10-01-28

All Science Journal Classification (ASJC) codes

Electronic, Optical and Magnetic Materials
Condensed Matter Physics
Mechanical Engineering
Electrical and Electronic Engineering

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1109/MEMSYS.2010.5442372

Cite this

Lee, W. B., Chen, Y. H., Lin, H. I., Huang, C. J., Shiesh, S. C., & Lee, G. B. (2010). A microfluidic system integrated with optical detection devices for automatic detection of C-reactive protein. In MEMS 2010 - The 23rd IEEE International Conference on Micro Electro Mechanical Systems, Technical Digest (pp. 979-982). Article 5442372 (Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)). https://doi.org/10.1109/MEMSYS.2010.5442372

Lee, Wen Bin ; Chen, Yin He ; Lin, Hsin I. et al. / A microfluidic system integrated with optical detection devices for automatic detection of C-reactive protein. MEMS 2010 - The 23rd IEEE International Conference on Micro Electro Mechanical Systems, Technical Digest. 2010. pp. 979-982 (Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)).

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title = "A microfluidic system integrated with optical detection devices for automatic detection of C-reactive protein",

abstract = "C-reactive protein (CRP) is commonly known as a clinical marker, which has been identified as an independent predictor for cardiovascular diseases such as heart attack and stroke. In this study, a new microfluidic system for automatic measurement of CRP is reported. The entire process including sample pre-treatment, incubation between target CRP and anti-CRP antibody, developing process and detection can be automatically performed on a single chip. In addition, chemiluminescent signal was measured by using a home-made luminometer to quantify the concentration of CRP. When compared to previous works, not only can the new chip perform the entire process by integrating a new micro-injector, but a new CRP-specific DNA aptamer was also used for CRP measurement. Experimental data showed that the developed system can automate the entire process within 30 minutes with a detection limit of 0.0125 mgL-1, which was superior to previous works.",

author = "Lee, {Wen Bin} and Chen, {Yin He} and Lin, {Hsin I.} and Huang, {Chao June} and Shiesh, {Shu Chu} and Lee, {Gwo Bin}",

year = "2010",

month = jun,

day = "1",

doi = "10.1109/MEMSYS.2010.5442372",

language = "English",

isbn = "9781424457649",

series = "Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)",

pages = "979--982",

booktitle = "MEMS 2010 - The 23rd IEEE International Conference on Micro Electro Mechanical Systems, Technical Digest",

note = "23rd IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2010 ; Conference date: 24-01-2010 Through 28-01-2010",

}

Lee, WB, Chen, YH, Lin, HI, Huang, CJ, Shiesh, SC & Lee, GB 2010, A microfluidic system integrated with optical detection devices for automatic detection of C-reactive protein. in MEMS 2010 - The 23rd IEEE International Conference on Micro Electro Mechanical Systems, Technical Digest., 5442372, Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS), pp. 979-982, 23rd IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2010, Hong Kong, China, 10-01-24. https://doi.org/10.1109/MEMSYS.2010.5442372

A microfluidic system integrated with optical detection devices for automatic detection of C-reactive protein. / Lee, Wen Bin; Chen, Yin He; Lin, Hsin I. et al.
MEMS 2010 - The 23rd IEEE International Conference on Micro Electro Mechanical Systems, Technical Digest. 2010. p. 979-982 5442372 (Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

TY - GEN

T1 - A microfluidic system integrated with optical detection devices for automatic detection of C-reactive protein

AU - Lee, Wen Bin

AU - Chen, Yin He

AU - Lin, Hsin I.

AU - Huang, Chao June

AU - Shiesh, Shu Chu

AU - Lee, Gwo Bin

PY - 2010/6/1

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N2 - C-reactive protein (CRP) is commonly known as a clinical marker, which has been identified as an independent predictor for cardiovascular diseases such as heart attack and stroke. In this study, a new microfluidic system for automatic measurement of CRP is reported. The entire process including sample pre-treatment, incubation between target CRP and anti-CRP antibody, developing process and detection can be automatically performed on a single chip. In addition, chemiluminescent signal was measured by using a home-made luminometer to quantify the concentration of CRP. When compared to previous works, not only can the new chip perform the entire process by integrating a new micro-injector, but a new CRP-specific DNA aptamer was also used for CRP measurement. Experimental data showed that the developed system can automate the entire process within 30 minutes with a detection limit of 0.0125 mgL-1, which was superior to previous works.

AB - C-reactive protein (CRP) is commonly known as a clinical marker, which has been identified as an independent predictor for cardiovascular diseases such as heart attack and stroke. In this study, a new microfluidic system for automatic measurement of CRP is reported. The entire process including sample pre-treatment, incubation between target CRP and anti-CRP antibody, developing process and detection can be automatically performed on a single chip. In addition, chemiluminescent signal was measured by using a home-made luminometer to quantify the concentration of CRP. When compared to previous works, not only can the new chip perform the entire process by integrating a new micro-injector, but a new CRP-specific DNA aptamer was also used for CRP measurement. Experimental data showed that the developed system can automate the entire process within 30 minutes with a detection limit of 0.0125 mgL-1, which was superior to previous works.

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Lee WB, Chen YH, Lin HI, Huang CJ, Shiesh SC, Lee GB. A microfluidic system integrated with optical detection devices for automatic detection of C-reactive protein. In MEMS 2010 - The 23rd IEEE International Conference on Micro Electro Mechanical Systems, Technical Digest. 2010. p. 979-982. 5442372. (Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)). doi: 10.1109/MEMSYS.2010.5442372

A microfluidic system integrated with optical detection devices for automatic detection of C-reactive protein

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