A microfluidic system integrated with optical detection devices for automatic detection of C-reactive protein

Wen Bin Lee, Yin He Chen, Hsin I. Lin, Chao June Huang, Shu Chu Shiesh, Gwo Bin Lee

Research output: Chapter in Book/Report/Conference proceedingConference contribution

2 Citations (Scopus)

Abstract

C-reactive protein (CRP) is commonly known as a clinical marker, which has been identified as an independent predictor for cardiovascular diseases such as heart attack and stroke. In this study, a new microfluidic system for automatic measurement of CRP is reported. The entire process including sample pre-treatment, incubation between target CRP and anti-CRP antibody, developing process and detection can be automatically performed on a single chip. In addition, chemiluminescent signal was measured by using a home-made luminometer to quantify the concentration of CRP. When compared to previous works, not only can the new chip perform the entire process by integrating a new micro-injector, but a new CRP-specific DNA aptamer was also used for CRP measurement. Experimental data showed that the developed system can automate the entire process within 30 minutes with a detection limit of 0.0125 mgL-1, which was superior to previous works.

Original languageEnglish
Title of host publicationMEMS 2010 - The 23rd IEEE International Conference on Micro Electro Mechanical Systems, Technical Digest
Pages979-982
Number of pages4
DOIs
Publication statusPublished - 2010 Jun 1
Event23rd IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2010 - Hong Kong, China
Duration: 2010 Jan 242010 Jan 28

Publication series

NameProceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)
ISSN (Print)1084-6999

Other

Other23rd IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2010
CountryChina
CityHong Kong
Period10-01-2410-01-28

Fingerprint

Microfluidics
C-Reactive Protein
proteins
Proteins
chips
Nucleotide Aptamers
biomarkers
strokes
antibodies
injectors
Antibodies
pretreatment
attack
DNA
deoxyribonucleic acid
predictions

All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Mechanical Engineering
  • Electrical and Electronic Engineering

Cite this

Lee, W. B., Chen, Y. H., Lin, H. I., Huang, C. J., Shiesh, S. C., & Lee, G. B. (2010). A microfluidic system integrated with optical detection devices for automatic detection of C-reactive protein. In MEMS 2010 - The 23rd IEEE International Conference on Micro Electro Mechanical Systems, Technical Digest (pp. 979-982). [5442372] (Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)). https://doi.org/10.1109/MEMSYS.2010.5442372
Lee, Wen Bin ; Chen, Yin He ; Lin, Hsin I. ; Huang, Chao June ; Shiesh, Shu Chu ; Lee, Gwo Bin. / A microfluidic system integrated with optical detection devices for automatic detection of C-reactive protein. MEMS 2010 - The 23rd IEEE International Conference on Micro Electro Mechanical Systems, Technical Digest. 2010. pp. 979-982 (Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)).
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abstract = "C-reactive protein (CRP) is commonly known as a clinical marker, which has been identified as an independent predictor for cardiovascular diseases such as heart attack and stroke. In this study, a new microfluidic system for automatic measurement of CRP is reported. The entire process including sample pre-treatment, incubation between target CRP and anti-CRP antibody, developing process and detection can be automatically performed on a single chip. In addition, chemiluminescent signal was measured by using a home-made luminometer to quantify the concentration of CRP. When compared to previous works, not only can the new chip perform the entire process by integrating a new micro-injector, but a new CRP-specific DNA aptamer was also used for CRP measurement. Experimental data showed that the developed system can automate the entire process within 30 minutes with a detection limit of 0.0125 mgL-1, which was superior to previous works.",
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Lee, WB, Chen, YH, Lin, HI, Huang, CJ, Shiesh, SC & Lee, GB 2010, A microfluidic system integrated with optical detection devices for automatic detection of C-reactive protein. in MEMS 2010 - The 23rd IEEE International Conference on Micro Electro Mechanical Systems, Technical Digest., 5442372, Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS), pp. 979-982, 23rd IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2010, Hong Kong, China, 10-01-24. https://doi.org/10.1109/MEMSYS.2010.5442372

A microfluidic system integrated with optical detection devices for automatic detection of C-reactive protein. / Lee, Wen Bin; Chen, Yin He; Lin, Hsin I.; Huang, Chao June; Shiesh, Shu Chu; Lee, Gwo Bin.

MEMS 2010 - The 23rd IEEE International Conference on Micro Electro Mechanical Systems, Technical Digest. 2010. p. 979-982 5442372 (Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

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Lee WB, Chen YH, Lin HI, Huang CJ, Shiesh SC, Lee GB. A microfluidic system integrated with optical detection devices for automatic detection of C-reactive protein. In MEMS 2010 - The 23rd IEEE International Conference on Micro Electro Mechanical Systems, Technical Digest. 2010. p. 979-982. 5442372. (Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)). https://doi.org/10.1109/MEMSYS.2010.5442372