A multi-functional electrochemical sensing system using microfluidic technology for the detection of urea and creatinine

Chao June Huang, Jiun Lin Lin, Ping Hong Chen, Mei-Jywan Syu, Gwo Bin Lee

Research output: Contribution to journalArticlepeer-review

19 Citations (Scopus)

Abstract

This study presents a new microfluidic system capable of precise measurements of two important biomarkers, urea and creatinine, automatically. In clinical applications, high levels of these two biomarkers are early indicators of nephropathy or renal failure and should be monitored on a regular basis. The microfluidic system is composed of a microfluidic chip, a control circuit system, a compressed air source and several electromagnetic valves to form a handheld system. The microfluidic chip is fabricated by using micro-electromechanical systems and microfluidic techniques comprising electrochemical sensor arrays and polydimethylsiloxane-based microfluidic structures such as micropumps/micromixers, normally closed valves and microchannels. The microfluidic system performs a variety of critical processes including sample pretreatment, mixing, transportation and detection on a single chip. The experimental results show that the entire procedure takes approximately 40min, which is much faster than the traditional method (more than 6h). Furthermore, the total sample volume consumed in each operation is only 0.1mL, which is significantly less than that required in a large system (5mL). The developed automatic microfluidic system may provide a powerful platform for further clinical applications.

Original languageEnglish
Pages (from-to)931-938
Number of pages8
JournalElectrophoresis
Volume32
Issue number8
DOIs
Publication statusPublished - 2011 Apr 1

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Biochemistry
  • Clinical Biochemistry

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