TY - JOUR
T1 - A new method for detection of endotoxin on polymyxin B-immobilized gold electrodes
AU - Ding, Shinn Jyh
AU - Chang, Bin Wha
AU - Wu, Ching Chou
AU - Chen, Chun Ji
AU - Chang, Hsien Chang
N1 - Funding Information:
The authors thank the Center for Micro/Nano Technology Research and the Center for Bioscience and Biotechnology, National Cheng Kung University, Tainan, Taiwan, for equipment access and technical support. Finance supported by the NSC fund (94-2320-B-006-031) is gratefully acknowledged.
PY - 2007/5
Y1 - 2007/5
N2 - Endotoxin can lead to irreversible shock and death, underlying the necessity for development of facile, rapid, sensitive and in situ methods to detect endotoxin. We used electrochemical impedance spectroscopy (EIS) to detect interaction of endotoxin with polymyxin B (PmB) immobilized on 4,4-dithiodibutyric acid coated gold electrodes. An equivalent circuit model with a constant phase element was used to interpret the obtained impedance spectra. The results indicated that the gold electrode after electrochemical polishing pretreatment in both 2-(N-Morpholino) ethanesulfonic acid (MES) and HClO4 solutions had a more effective surface state than that treated in H2SO4 solution. The changes in capacitance associated with a constant phase element on the PmB-modified electrodes were more sensitive, compared to those in the charge-transfer resistance, for detecting endotoxin over the 0.2-0.8 ng/mL concentrations. The activated and modified gold electrodes might be used to EIS detect endotoxin in real-time.
AB - Endotoxin can lead to irreversible shock and death, underlying the necessity for development of facile, rapid, sensitive and in situ methods to detect endotoxin. We used electrochemical impedance spectroscopy (EIS) to detect interaction of endotoxin with polymyxin B (PmB) immobilized on 4,4-dithiodibutyric acid coated gold electrodes. An equivalent circuit model with a constant phase element was used to interpret the obtained impedance spectra. The results indicated that the gold electrode after electrochemical polishing pretreatment in both 2-(N-Morpholino) ethanesulfonic acid (MES) and HClO4 solutions had a more effective surface state than that treated in H2SO4 solution. The changes in capacitance associated with a constant phase element on the PmB-modified electrodes were more sensitive, compared to those in the charge-transfer resistance, for detecting endotoxin over the 0.2-0.8 ng/mL concentrations. The activated and modified gold electrodes might be used to EIS detect endotoxin in real-time.
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U2 - 10.1016/j.elecom.2006.12.029
DO - 10.1016/j.elecom.2006.12.029
M3 - Article
AN - SCOPUS:34247342855
VL - 9
SP - 1206
EP - 1211
JO - Electrochemistry Communications
JF - Electrochemistry Communications
SN - 1388-2481
IS - 5
ER -