Abstract
Mitochondria DNA (mtDNA) plays an important role in both aging-associated degenerative diseases and cancers. In this study, a new microfluidic system integrating functional components including a mtDNA extraction module, a polymerase chain reaction (PCR) module and a micro-capillary electrophoresis (MCE) module has been developed. A new approach to vertically integrate these three modules has been reported. A cell lysis device, pneumatic micropumps, and an active micromixer were first used for fast extraction of mtDNA from cells. Then, the PCR and the MCE modules were used for nucleic acid amplification and separation of mtDNA. Experimental data showed that the deletion of mtDNA can be automatically detected with this approach.
| Original language | English |
|---|---|
| Title of host publication | 2011 IEEE 24th International Conference on Micro Electro Mechanical Systems, MEMS 2011 |
| Pages | 193-196 |
| Number of pages | 4 |
| DOIs | |
| Publication status | Published - 2011 Apr 13 |
| Event | 24th IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2011 - Cancun, Mexico Duration: 2011 Jan 23 → 2011 Jan 27 |
Publication series
| Name | Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS) |
|---|---|
| ISSN (Print) | 1084-6999 |
Other
| Other | 24th IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2011 |
|---|---|
| Country | Mexico |
| City | Cancun |
| Period | 11-01-23 → 11-01-27 |
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All Science Journal Classification (ASJC) codes
- Electronic, Optical and Magnetic Materials
- Condensed Matter Physics
- Mechanical Engineering
- Electrical and Electronic Engineering
Cite this
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A new microfluid system for mitochondrial DNA extraction and analysis. / Chang, Chen Min; Chiou, Li Fang; Lin, Chun Che; Shieh, Dar Bin; Lee, Gwo Bin.
2011 IEEE 24th International Conference on Micro Electro Mechanical Systems, MEMS 2011. 2011. p. 193-196 5734394 (Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)).Research output: Chapter in Book/Report/Conference proceeding › Conference contribution
TY - GEN
T1 - A new microfluid system for mitochondrial DNA extraction and analysis
AU - Chang, Chen Min
AU - Chiou, Li Fang
AU - Lin, Chun Che
AU - Shieh, Dar Bin
AU - Lee, Gwo Bin
PY - 2011/4/13
Y1 - 2011/4/13
N2 - Mitochondria DNA (mtDNA) plays an important role in both aging-associated degenerative diseases and cancers. In this study, a new microfluidic system integrating functional components including a mtDNA extraction module, a polymerase chain reaction (PCR) module and a micro-capillary electrophoresis (MCE) module has been developed. A new approach to vertically integrate these three modules has been reported. A cell lysis device, pneumatic micropumps, and an active micromixer were first used for fast extraction of mtDNA from cells. Then, the PCR and the MCE modules were used for nucleic acid amplification and separation of mtDNA. Experimental data showed that the deletion of mtDNA can be automatically detected with this approach.
AB - Mitochondria DNA (mtDNA) plays an important role in both aging-associated degenerative diseases and cancers. In this study, a new microfluidic system integrating functional components including a mtDNA extraction module, a polymerase chain reaction (PCR) module and a micro-capillary electrophoresis (MCE) module has been developed. A new approach to vertically integrate these three modules has been reported. A cell lysis device, pneumatic micropumps, and an active micromixer were first used for fast extraction of mtDNA from cells. Then, the PCR and the MCE modules were used for nucleic acid amplification and separation of mtDNA. Experimental data showed that the deletion of mtDNA can be automatically detected with this approach.
UR - http://www.scopus.com/inward/record.url?scp=79953778293&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=79953778293&partnerID=8YFLogxK
U2 - 10.1109/MEMSYS.2011.5734394
DO - 10.1109/MEMSYS.2011.5734394
M3 - Conference contribution
AN - SCOPUS:79953778293
SN - 9781424496327
T3 - Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)
SP - 193
EP - 196
BT - 2011 IEEE 24th International Conference on Micro Electro Mechanical Systems, MEMS 2011
ER -