A new microfluid system for mitochondrial DNA extraction and analysis

Chen Min Chang, Li Fang Chiou, Chun Che Lin, Dar Bin Shieh, Gwo Bin Lee

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Mitochondria DNA (mtDNA) plays an important role in both aging-associated degenerative diseases and cancers. In this study, a new microfluidic system integrating functional components including a mtDNA extraction module, a polymerase chain reaction (PCR) module and a micro-capillary electrophoresis (MCE) module has been developed. A new approach to vertically integrate these three modules has been reported. A cell lysis device, pneumatic micropumps, and an active micromixer were first used for fast extraction of mtDNA from cells. Then, the PCR and the MCE modules were used for nucleic acid amplification and separation of mtDNA. Experimental data showed that the deletion of mtDNA can be automatically detected with this approach.

Original languageEnglish
Title of host publication2011 IEEE 24th International Conference on Micro Electro Mechanical Systems, MEMS 2011
Pages193-196
Number of pages4
DOIs
Publication statusPublished - 2011 Apr 13
Event24th IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2011 - Cancun, Mexico
Duration: 2011 Jan 232011 Jan 27

Publication series

NameProceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)
ISSN (Print)1084-6999

Other

Other24th IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2011
CountryMexico
CityCancun
Period11-01-2311-01-27

Fingerprint

Mitochondria
mitochondria
Mitochondrial DNA
DNA
deoxyribonucleic acid
modules
polymerase chain reaction
Capillary electrophoresis
Polymerase chain reaction
electrophoresis
deletion
pneumatics
Nucleic acids
nucleic acids
cells
Microfluidics
Pneumatics
Nucleic Acids
Amplification
Aging of materials

All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Mechanical Engineering
  • Electrical and Electronic Engineering

Cite this

Chang, C. M., Chiou, L. F., Lin, C. C., Shieh, D. B., & Lee, G. B. (2011). A new microfluid system for mitochondrial DNA extraction and analysis. In 2011 IEEE 24th International Conference on Micro Electro Mechanical Systems, MEMS 2011 (pp. 193-196). [5734394] (Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)). https://doi.org/10.1109/MEMSYS.2011.5734394
Chang, Chen Min ; Chiou, Li Fang ; Lin, Chun Che ; Shieh, Dar Bin ; Lee, Gwo Bin. / A new microfluid system for mitochondrial DNA extraction and analysis. 2011 IEEE 24th International Conference on Micro Electro Mechanical Systems, MEMS 2011. 2011. pp. 193-196 (Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)).
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Chang, CM, Chiou, LF, Lin, CC, Shieh, DB & Lee, GB 2011, A new microfluid system for mitochondrial DNA extraction and analysis. in 2011 IEEE 24th International Conference on Micro Electro Mechanical Systems, MEMS 2011., 5734394, Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS), pp. 193-196, 24th IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2011, Cancun, Mexico, 11-01-23. https://doi.org/10.1109/MEMSYS.2011.5734394

A new microfluid system for mitochondrial DNA extraction and analysis. / Chang, Chen Min; Chiou, Li Fang; Lin, Chun Che; Shieh, Dar Bin; Lee, Gwo Bin.

2011 IEEE 24th International Conference on Micro Electro Mechanical Systems, MEMS 2011. 2011. p. 193-196 5734394 (Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

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Chang CM, Chiou LF, Lin CC, Shieh DB, Lee GB. A new microfluid system for mitochondrial DNA extraction and analysis. In 2011 IEEE 24th International Conference on Micro Electro Mechanical Systems, MEMS 2011. 2011. p. 193-196. 5734394. (Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)). https://doi.org/10.1109/MEMSYS.2011.5734394