A new microfluid system for mitochondrial DNA extraction and analysis

Chen Min Chang; Li Fang Chiou; Chun Che Lin; Dar Bin Shieh; Gwo Bin Lee

doi:10.1109/MEMSYS.2011.5734394

A new microfluid system for mitochondrial DNA extraction and analysis

Chen Min Chang, Li Fang Chiou, Chun Che Lin, Dar Bin Shieh, Gwo Bin Lee

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

Mitochondria DNA (mtDNA) plays an important role in both aging-associated degenerative diseases and cancers. In this study, a new microfluidic system integrating functional components including a mtDNA extraction module, a polymerase chain reaction (PCR) module and a micro-capillary electrophoresis (MCE) module has been developed. A new approach to vertically integrate these three modules has been reported. A cell lysis device, pneumatic micropumps, and an active micromixer were first used for fast extraction of mtDNA from cells. Then, the PCR and the MCE modules were used for nucleic acid amplification and separation of mtDNA. Experimental data showed that the deletion of mtDNA can be automatically detected with this approach.

Original language	English
Title of host publication	2011 IEEE 24th International Conference on Micro Electro Mechanical Systems, MEMS 2011
Pages	193-196
Number of pages	4
DOIs	https://doi.org/10.1109/MEMSYS.2011.5734394
Publication status	Published - 2011 Apr 13
Event	24th IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2011 - Cancun, Mexico Duration: 2011 Jan 23 → 2011 Jan 27

Publication series

Name	Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)
ISSN (Print)	1084-6999

Other

Other	24th IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2011
Country	Mexico
City	Cancun
Period	11-01-23 → 11-01-27

All Science Journal Classification (ASJC) codes

Electronic, Optical and Magnetic Materials
Condensed Matter Physics
Mechanical Engineering
Electrical and Electronic Engineering

Access to Document

10.1109/MEMSYS.2011.5734394

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Chang, C. M., Chiou, L. F., Lin, C. C., Shieh, D. B., & Lee, G. B. (2011). A new microfluid system for mitochondrial DNA extraction and analysis. In 2011 IEEE 24th International Conference on Micro Electro Mechanical Systems, MEMS 2011 (pp. 193-196). [5734394] (Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)). https://doi.org/10.1109/MEMSYS.2011.5734394

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title = "A new microfluid system for mitochondrial DNA extraction and analysis",

abstract = "Mitochondria DNA (mtDNA) plays an important role in both aging-associated degenerative diseases and cancers. In this study, a new microfluidic system integrating functional components including a mtDNA extraction module, a polymerase chain reaction (PCR) module and a micro-capillary electrophoresis (MCE) module has been developed. A new approach to vertically integrate these three modules has been reported. A cell lysis device, pneumatic micropumps, and an active micromixer were first used for fast extraction of mtDNA from cells. Then, the PCR and the MCE modules were used for nucleic acid amplification and separation of mtDNA. Experimental data showed that the deletion of mtDNA can be automatically detected with this approach.",

author = "Chang, {Chen Min} and Chiou, {Li Fang} and Lin, {Chun Che} and Shieh, {Dar Bin} and Lee, {Gwo Bin}",

year = "2011",

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day = "13",

doi = "10.1109/MEMSYS.2011.5734394",

language = "English",

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series = "Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)",

pages = "193--196",

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Chang, CM, Chiou, LF, Lin, CC, Shieh, DB & Lee, GB 2011, A new microfluid system for mitochondrial DNA extraction and analysis. in 2011 IEEE 24th International Conference on Micro Electro Mechanical Systems, MEMS 2011., 5734394, Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS), pp. 193-196, 24th IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2011, Cancun, Mexico, 11-01-23. https://doi.org/10.1109/MEMSYS.2011.5734394

A new microfluid system for mitochondrial DNA extraction and analysis. / Chang, Chen Min; Chiou, Li Fang; Lin, Chun Che; Shieh, Dar Bin; Lee, Gwo Bin.

2011 IEEE 24th International Conference on Micro Electro Mechanical Systems, MEMS 2011. 2011. p. 193-196 5734394 (Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

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AB - Mitochondria DNA (mtDNA) plays an important role in both aging-associated degenerative diseases and cancers. In this study, a new microfluidic system integrating functional components including a mtDNA extraction module, a polymerase chain reaction (PCR) module and a micro-capillary electrophoresis (MCE) module has been developed. A new approach to vertically integrate these three modules has been reported. A cell lysis device, pneumatic micropumps, and an active micromixer were first used for fast extraction of mtDNA from cells. Then, the PCR and the MCE modules were used for nucleic acid amplification and separation of mtDNA. Experimental data showed that the deletion of mtDNA can be automatically detected with this approach.

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Chang CM, Chiou LF, Lin CC, Shieh DB, Lee GB. A new microfluid system for mitochondrial DNA extraction and analysis. In 2011 IEEE 24th International Conference on Micro Electro Mechanical Systems, MEMS 2011. 2011. p. 193-196. 5734394. (Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)). https://doi.org/10.1109/MEMSYS.2011.5734394