A new platform for manipulating a single dna molecule by using opticallay-induced dielectrophoresis

Yen Heng Lin, Chen Min Chang, Gwo Bin Lee

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Citation (Scopus)

Abstract

This paper presents a new platform capable of manipulating a single DNA molecule based on optically-induced dielectrophoretic (ODEP) forces. The ends of λ-phage DNA were bound with polystyrene beads which can be manipulated by optical images generated by a digital projector. The manipulation process of fluorescent-labeled DNA was observed in real time under the ODEP operation. Two kinds of DNA manipulation were conducted including DNA stretching and revolving through the computer-controlled optical images. The stretching force was also measured to estimate the manipulation ability of DNA molecule in this platform. The maximum stretching force was estimated to be about 49.73 pN when binding with 10 μm polystyrene beads. The developed platform may be a promising tool for single DNA molecule investigation.

Original languageEnglish
Title of host publicationTRANSDUCERS 2009 - 15th International Conference on Solid-State Sensors, Actuators and Microsystems
Pages1590-1593
Number of pages4
DOIs
Publication statusPublished - 2009 Dec 11
EventTRANSDUCERS 2009 - 15th International Conference on Solid-State Sensors, Actuators and Microsystems - Denver, CO, United States
Duration: 2009 Jun 212009 Jun 25

Publication series

NameTRANSDUCERS 2009 - 15th International Conference on Solid-State Sensors, Actuators and Microsystems

Other

OtherTRANSDUCERS 2009 - 15th International Conference on Solid-State Sensors, Actuators and Microsystems
CountryUnited States
CityDenver, CO
Period09-06-2109-06-25

All Science Journal Classification (ASJC) codes

  • Hardware and Architecture
  • Electrical and Electronic Engineering

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