TY - JOUR
T1 - A new protein A assay based on Raman reporter labeled immunogold nanoparticles
AU - Lin, Chi Chang
AU - Yang, Ying Mei
AU - Chen, Yan Fu
AU - Yang, Tzyy Schiuan
AU - Chang, Hsien Chang
N1 - Funding Information:
The authors thank the Center for Micro/Nano Technology Research and the Center for Bioscience and Biotechnology, National Cheng Kung University, Tainan, Taiwan, for equipment access and technical supports. This work was supported by the Landmark Project of National Cheng Kung University (A0011, A0052), the Technology Development Program for Academia of Ministry of Economic Affairs (96-EC-17-A-10-S1-013) and by the National Science Council of the Republic of China (NSC 95-2221-E-006 -215-). We also thank Prof. Hong-Ping Lin's group for the assistance in obtaining the TEM images.
PY - 2008/10/15
Y1 - 2008/10/15
N2 - A unique, sensitive, highly specific, and photobleaching-resistant immunoassay system utilizing gold nanoparticles and surface-enhanced Raman scattering (SERS) is described. This new system, featuring a capability of bifunctional analysis, is manufactured by chemisorption of antibody immunoglobulin G (IgG) on gold nanoparticles (AuNP), followed by coupling the Raman-active reporter molecule, 5,5′-dithiobis(2-nitrobenzoic acid) (DTNB) to the surface of IgG-AuNP. The adsorbed DTNB molecules exhibit strong Raman signals via both electromagnetic and chemical enhancement. The narrow spectral widths and high photostability assure the system to be an excellent detection label. This SERS-based immunoassay is applied to the detection of protein A, which is a specific surface antigen of Staphylococcus aureus. A working curve is obtained by plotting the intensity of the SERS signal of symmetric NO2 stretching of DTNB at 1333 cm-1 versus the concentration of the analyte (antigen). A dynamic range of two to three orders of magnitude and a detection limit of 1 pg/mL of protein A are achieved.
AB - A unique, sensitive, highly specific, and photobleaching-resistant immunoassay system utilizing gold nanoparticles and surface-enhanced Raman scattering (SERS) is described. This new system, featuring a capability of bifunctional analysis, is manufactured by chemisorption of antibody immunoglobulin G (IgG) on gold nanoparticles (AuNP), followed by coupling the Raman-active reporter molecule, 5,5′-dithiobis(2-nitrobenzoic acid) (DTNB) to the surface of IgG-AuNP. The adsorbed DTNB molecules exhibit strong Raman signals via both electromagnetic and chemical enhancement. The narrow spectral widths and high photostability assure the system to be an excellent detection label. This SERS-based immunoassay is applied to the detection of protein A, which is a specific surface antigen of Staphylococcus aureus. A working curve is obtained by plotting the intensity of the SERS signal of symmetric NO2 stretching of DTNB at 1333 cm-1 versus the concentration of the analyte (antigen). A dynamic range of two to three orders of magnitude and a detection limit of 1 pg/mL of protein A are achieved.
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U2 - 10.1016/j.bios.2008.03.035
DO - 10.1016/j.bios.2008.03.035
M3 - Article
C2 - 18468881
AN - SCOPUS:48149097868
SN - 0956-5663
VL - 24
SP - 178
EP - 183
JO - Biosensors and Bioelectronics
JF - Biosensors and Bioelectronics
IS - 2
ER -