TY - JOUR
T1 - A newly developed optical biochip for bacteria detection based on DNA hybridization
AU - Yeh, Chia Hsien
AU - Chang, Yu Huai
AU - Lin, Hong Ping
AU - Chang, Tsung Chain
AU - Lin, Yu Cheng
N1 - Funding Information:
The authors would like to thank the Center for Micro/Nano Technology, National Cheng Kung University, Tainan, Taiwan, ROC, for access to equipment and technical support. Funding from the Ministry of Education and the National Science Council of Taiwan , ROC under grants NSC 99-2221-E-006-203-MY3 and 98-EC-17-A-19-S1-133 are gratefully acknowledged. This paper is also partially supported by “Aim for the Top University Plan” of the National Chiao Tung University and Ministry of Education, Taiwan, ROC.
PY - 2012/1/3
Y1 - 2012/1/3
N2 - This paper reports a bacterial optical biochip based on a developed DNA hybridization detection method using an optical measurement. Because the occurrence of bacteremia caused by Acinetobacter baumannii is high in hospitals worldwide, this species was chosen as the source of the DNA samples. Our strategy is based on a developed DNA hybridization detection method which uses probes, PCR-amplified bacterial DNA with biotin labeled primers as well as detection enhancement using gold-streptavidin nanoparticles and an Ag +-hydroquinone solution. Because the gold nanoparticles catalyze silver ions to silver metals by using hydroquinone, the gradually precipitated silver metal causes differences in the optical detection signals, which can be easily observed with the naked eye or with an optical instrument (such as a flatbed scanner). The relationships between sample concentration and detection signal are discussed, and the detection limit for Acinetobacter baumannii genomic DNA is 8.25 ng/mL (1.07 × 10 10 copy/mL). Probe specificity was analyzed by detecting various species of bacteria, various strains of a single species, and various species of a single genus. The DNA hybridization detection method for the bacterial optical biochip presented in this work is easy, rapid, convenient, and promising for detecting bacterial infections and in clinical diagnoses.
AB - This paper reports a bacterial optical biochip based on a developed DNA hybridization detection method using an optical measurement. Because the occurrence of bacteremia caused by Acinetobacter baumannii is high in hospitals worldwide, this species was chosen as the source of the DNA samples. Our strategy is based on a developed DNA hybridization detection method which uses probes, PCR-amplified bacterial DNA with biotin labeled primers as well as detection enhancement using gold-streptavidin nanoparticles and an Ag +-hydroquinone solution. Because the gold nanoparticles catalyze silver ions to silver metals by using hydroquinone, the gradually precipitated silver metal causes differences in the optical detection signals, which can be easily observed with the naked eye or with an optical instrument (such as a flatbed scanner). The relationships between sample concentration and detection signal are discussed, and the detection limit for Acinetobacter baumannii genomic DNA is 8.25 ng/mL (1.07 × 10 10 copy/mL). Probe specificity was analyzed by detecting various species of bacteria, various strains of a single species, and various species of a single genus. The DNA hybridization detection method for the bacterial optical biochip presented in this work is easy, rapid, convenient, and promising for detecting bacterial infections and in clinical diagnoses.
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U2 - 10.1016/j.snb.2011.10.016
DO - 10.1016/j.snb.2011.10.016
M3 - Article
AN - SCOPUS:84856212866
SN - 0925-4005
VL - 161
SP - 1168
EP - 1175
JO - Sensors and Actuators, B: Chemical
JF - Sensors and Actuators, B: Chemical
IS - 1
ER -