A Non-biotin Polymerized Horseradish-peroxidase Method for the Immunohistochemical Diagnosis of Canine Distemper

C. T. Liang, L. L. Chueh, V. F. Pang, Y. X. Zhuo, S. C. Liang, Chun-Keung Yu, H. Chiang, C. C. Lee, C. H. Liu

Research output: Contribution to journalArticlepeer-review

12 Citations (Scopus)

Abstract

This report describes a modified non-biotin polymerized horseradish peroxidase (HRP) immunohistochemical method for the diagnosis of canine distemper virus (CDV) infection from formalin-fixed, paraffin wax-embedded tissues. This method confirmed infection in seven of eight (87.5%) suspected cases. Labelled CDV antigen was observed in the following sites: cerebrum, cerebellum, meninges, glial cells, neurons, vascular endothelium, periventricular areas and pericytes, and choroid plexus; grey and white matter and central canal of the spinal cord; renal pelvis and tubular epithelium, and urinary bladder epithelium; macrophages and lymphocytes in splenic white pulp and lymph nodes; skin epidermis; bronchiolar epithelium and alveolar macrophages; hepatic Kupffer cells, and gastric and intestinal mucosal epithelium; stratified squamous epithelium of the tongue and oesophagus. With the non-biotin HRP detection system, pretreatment by autoclaving followed by microwave heating gave better labelling results than did microwave pretreatment alone. No obvious difference was noted between the labelling results produced by the non-biotin HRP detection system and the Super Sensitive™ Link-Label IHC detection system.

Original languageEnglish
Pages (from-to)57-64
Number of pages8
JournalJournal of Comparative Pathology
Volume136
Issue number1
DOIs
Publication statusPublished - 2007 Jan 1

All Science Journal Classification (ASJC) codes

  • Pathology and Forensic Medicine
  • veterinary(all)

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