A nontoxic Pseudomonas exotoxin A induces active immunity and passive protective antibody against Pseudomonas exotoxin A intoxication

Tzong-Yueh Chen, Chia Po Lin, Chien Chang Loa, Tso Ling Chen, Huey Fang Shang, Jaulang Hwang, Cho Fat Hui

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Pseudomonas exotoxin A (PE) is one of the most potent cytotoxic agents produced by Pseudomonas aeruginosa. In this study, we examined the possibility of using PE with a deletion of 38 carboxyl-terminal amino acid residues, designated PE(ΔA576-613), for active immunization against PE- mediated disease. We first examined the toxic effects of PE and PE(Δ576-613) on 5- and 9-week-old ICR mice. The results show that the subcutaneous administration of PE(Δ576-613) at a dose of 250 μg was still nontoxic to 5- and 9-week-old ICR mice, while native PE was lethal at a dose of 0.5 and 1 μg, respectively. PE(Δ576-613) was then used to immunize ICR mice. The minimum dose of PE(Δ576-613) that could effectively induce anti-PE antibodies in 5- and 9-week-old ICR mice was found to be 250 ng. However, immunization with 250 ng PE(Δ576-613) failed to protect the immunized mice from a lethal dose of PE. The effective immunization dose of PE(Δ576-613) that could protect mice against a 2 μg PE challenge was found to be 15 μg. In addition, sera obtained from PE(Δ576-613)-immunized ICR mice were able to neutralize PE intoxication and effectively protect mice from PE. Thus, PE(Δ576-613) may be used as an alternative route to new PE vaccine development.

Original languageEnglish
Pages (from-to)357-363
Number of pages7
JournalJournal of biomedical science
Volume6
Issue number5
DOIs
Publication statusPublished - 1999 Jan 1

Fingerprint

Active Immunity
Antibodies
Inbred ICR Mouse
Immunization
Pseudomonas aeruginosa toxA protein

All Science Journal Classification (ASJC) codes

  • Endocrinology, Diabetes and Metabolism
  • Molecular Biology
  • Clinical Biochemistry
  • Cell Biology
  • Biochemistry, medical
  • Pharmacology (medical)

Cite this

Chen, Tzong-Yueh ; Lin, Chia Po ; Loa, Chien Chang ; Chen, Tso Ling ; Shang, Huey Fang ; Hwang, Jaulang ; Hui, Cho Fat. / A nontoxic Pseudomonas exotoxin A induces active immunity and passive protective antibody against Pseudomonas exotoxin A intoxication. In: Journal of biomedical science. 1999 ; Vol. 6, No. 5. pp. 357-363.
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abstract = "Pseudomonas exotoxin A (PE) is one of the most potent cytotoxic agents produced by Pseudomonas aeruginosa. In this study, we examined the possibility of using PE with a deletion of 38 carboxyl-terminal amino acid residues, designated PE(ΔA576-613), for active immunization against PE- mediated disease. We first examined the toxic effects of PE and PE(Δ576-613) on 5- and 9-week-old ICR mice. The results show that the subcutaneous administration of PE(Δ576-613) at a dose of 250 μg was still nontoxic to 5- and 9-week-old ICR mice, while native PE was lethal at a dose of 0.5 and 1 μg, respectively. PE(Δ576-613) was then used to immunize ICR mice. The minimum dose of PE(Δ576-613) that could effectively induce anti-PE antibodies in 5- and 9-week-old ICR mice was found to be 250 ng. However, immunization with 250 ng PE(Δ576-613) failed to protect the immunized mice from a lethal dose of PE. The effective immunization dose of PE(Δ576-613) that could protect mice against a 2 μg PE challenge was found to be 15 μg. In addition, sera obtained from PE(Δ576-613)-immunized ICR mice were able to neutralize PE intoxication and effectively protect mice from PE. Thus, PE(Δ576-613) may be used as an alternative route to new PE vaccine development.",
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A nontoxic Pseudomonas exotoxin A induces active immunity and passive protective antibody against Pseudomonas exotoxin A intoxication. / Chen, Tzong-Yueh; Lin, Chia Po; Loa, Chien Chang; Chen, Tso Ling; Shang, Huey Fang; Hwang, Jaulang; Hui, Cho Fat.

In: Journal of biomedical science, Vol. 6, No. 5, 01.01.1999, p. 357-363.

Research output: Contribution to journalArticle

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