Abstract
This paper reports a novel immunoassay using an electro-microchip to detect the immuno-reaction signal, a gold nanoparticles (AuNPs) as a label of antigen or antibody and as a catalyst for silver precipitation, and the silver enhancement reaction to magnify the detection signal. The LCR meter instrument, a new detection method for immunoassay, is used to detect the electric single in a sandwich format. The change of the resistance to corroborate immuno-reaction is detected, amplified and analyzed to construct this new immunoassay. Different sizes of the electrodes are designed to discuss the relationship between gap size and sensitivity. In addition, the specificity, sensitivity and the detection limit of the electro-immunoassay are discussed. Using electro-microchip, nanoparticle and silver enhancement provided a new immunoassay method.
| Original language | English |
|---|---|
| Title of host publication | Proceedings of the 33rd Annual Conference of the IEEE Industrial Electronics Society, IECON |
| Pages | 2966-2969 |
| Number of pages | 4 |
| DOIs | |
| Publication status | Published - 2007 Dec 1 |
| Event | 33rd Annual Conference of the IEEE Industrial Electronics Society, IECON - Taipei, Taiwan Duration: 2007 Nov 5 → 2007 Nov 8 |
Publication series
| Name | IECON Proceedings (Industrial Electronics Conference) |
|---|
Other
| Other | 33rd Annual Conference of the IEEE Industrial Electronics Society, IECON |
|---|---|
| Country | Taiwan |
| City | Taipei |
| Period | 07-11-05 → 07-11-08 |
Fingerprint
All Science Journal Classification (ASJC) codes
- Control and Systems Engineering
- Electrical and Electronic Engineering
Cite this
}
A novel and efficient immunoassay : Using electro-microchip, gold nanoparticle and silver enhancement. / Huang, Keng Shiang; Chen, Wei Ting; Lee, Sheng Ji; Yeh, Chia Hsien; Chang, Tsung Chain; Lin, Hong-Ping; Lin, Yu-Cheng.
Proceedings of the 33rd Annual Conference of the IEEE Industrial Electronics Society, IECON. 2007. p. 2966-2969 4459931 (IECON Proceedings (Industrial Electronics Conference)).Research output: Chapter in Book/Report/Conference proceeding › Conference contribution
TY - GEN
T1 - A novel and efficient immunoassay
T2 - Using electro-microchip, gold nanoparticle and silver enhancement
AU - Huang, Keng Shiang
AU - Chen, Wei Ting
AU - Lee, Sheng Ji
AU - Yeh, Chia Hsien
AU - Chang, Tsung Chain
AU - Lin, Hong-Ping
AU - Lin, Yu-Cheng
PY - 2007/12/1
Y1 - 2007/12/1
N2 - This paper reports a novel immunoassay using an electro-microchip to detect the immuno-reaction signal, a gold nanoparticles (AuNPs) as a label of antigen or antibody and as a catalyst for silver precipitation, and the silver enhancement reaction to magnify the detection signal. The LCR meter instrument, a new detection method for immunoassay, is used to detect the electric single in a sandwich format. The change of the resistance to corroborate immuno-reaction is detected, amplified and analyzed to construct this new immunoassay. Different sizes of the electrodes are designed to discuss the relationship between gap size and sensitivity. In addition, the specificity, sensitivity and the detection limit of the electro-immunoassay are discussed. Using electro-microchip, nanoparticle and silver enhancement provided a new immunoassay method.
AB - This paper reports a novel immunoassay using an electro-microchip to detect the immuno-reaction signal, a gold nanoparticles (AuNPs) as a label of antigen or antibody and as a catalyst for silver precipitation, and the silver enhancement reaction to magnify the detection signal. The LCR meter instrument, a new detection method for immunoassay, is used to detect the electric single in a sandwich format. The change of the resistance to corroborate immuno-reaction is detected, amplified and analyzed to construct this new immunoassay. Different sizes of the electrodes are designed to discuss the relationship between gap size and sensitivity. In addition, the specificity, sensitivity and the detection limit of the electro-immunoassay are discussed. Using electro-microchip, nanoparticle and silver enhancement provided a new immunoassay method.
UR - http://www.scopus.com/inward/record.url?scp=49949104346&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=49949104346&partnerID=8YFLogxK
U2 - 10.1109/IECON.2007.4459931
DO - 10.1109/IECON.2007.4459931
M3 - Conference contribution
SN - 1424407834
SN - 9781424407835
T3 - IECON Proceedings (Industrial Electronics Conference)
SP - 2966
EP - 2969
BT - Proceedings of the 33rd Annual Conference of the IEEE Industrial Electronics Society, IECON
ER -