A novel and efficient immunoassay

Using electro-microchip, gold nanoparticle and silver enhancement

Keng Shiang Huang, Wei Ting Chen, Sheng Ji Lee, Chia Hsien Yeh, Tsung Chain Chang, Hong-Ping Lin, Yu-Cheng Lin

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Citation (Scopus)

Abstract

This paper reports a novel immunoassay using an electro-microchip to detect the immuno-reaction signal, a gold nanoparticles (AuNPs) as a label of antigen or antibody and as a catalyst for silver precipitation, and the silver enhancement reaction to magnify the detection signal. The LCR meter instrument, a new detection method for immunoassay, is used to detect the electric single in a sandwich format. The change of the resistance to corroborate immuno-reaction is detected, amplified and analyzed to construct this new immunoassay. Different sizes of the electrodes are designed to discuss the relationship between gap size and sensitivity. In addition, the specificity, sensitivity and the detection limit of the electro-immunoassay are discussed. Using electro-microchip, nanoparticle and silver enhancement provided a new immunoassay method.

Original languageEnglish
Title of host publicationProceedings of the 33rd Annual Conference of the IEEE Industrial Electronics Society, IECON
Pages2966-2969
Number of pages4
DOIs
Publication statusPublished - 2007 Dec 1
Event33rd Annual Conference of the IEEE Industrial Electronics Society, IECON - Taipei, Taiwan
Duration: 2007 Nov 52007 Nov 8

Publication series

NameIECON Proceedings (Industrial Electronics Conference)

Other

Other33rd Annual Conference of the IEEE Industrial Electronics Society, IECON
CountryTaiwan
CityTaipei
Period07-11-0507-11-08

Fingerprint

Silver
Gold
Nanoparticles
Signal detection
Antigens
Antibodies
Labels
Electrodes
Catalysts

All Science Journal Classification (ASJC) codes

  • Control and Systems Engineering
  • Electrical and Electronic Engineering

Cite this

Huang, K. S., Chen, W. T., Lee, S. J., Yeh, C. H., Chang, T. C., Lin, H-P., & Lin, Y-C. (2007). A novel and efficient immunoassay: Using electro-microchip, gold nanoparticle and silver enhancement. In Proceedings of the 33rd Annual Conference of the IEEE Industrial Electronics Society, IECON (pp. 2966-2969). [4459931] (IECON Proceedings (Industrial Electronics Conference)). https://doi.org/10.1109/IECON.2007.4459931
Huang, Keng Shiang ; Chen, Wei Ting ; Lee, Sheng Ji ; Yeh, Chia Hsien ; Chang, Tsung Chain ; Lin, Hong-Ping ; Lin, Yu-Cheng. / A novel and efficient immunoassay : Using electro-microchip, gold nanoparticle and silver enhancement. Proceedings of the 33rd Annual Conference of the IEEE Industrial Electronics Society, IECON. 2007. pp. 2966-2969 (IECON Proceedings (Industrial Electronics Conference)).
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abstract = "This paper reports a novel immunoassay using an electro-microchip to detect the immuno-reaction signal, a gold nanoparticles (AuNPs) as a label of antigen or antibody and as a catalyst for silver precipitation, and the silver enhancement reaction to magnify the detection signal. The LCR meter instrument, a new detection method for immunoassay, is used to detect the electric single in a sandwich format. The change of the resistance to corroborate immuno-reaction is detected, amplified and analyzed to construct this new immunoassay. Different sizes of the electrodes are designed to discuss the relationship between gap size and sensitivity. In addition, the specificity, sensitivity and the detection limit of the electro-immunoassay are discussed. Using electro-microchip, nanoparticle and silver enhancement provided a new immunoassay method.",
author = "Huang, {Keng Shiang} and Chen, {Wei Ting} and Lee, {Sheng Ji} and Yeh, {Chia Hsien} and Chang, {Tsung Chain} and Hong-Ping Lin and Yu-Cheng Lin",
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Huang, KS, Chen, WT, Lee, SJ, Yeh, CH, Chang, TC, Lin, H-P & Lin, Y-C 2007, A novel and efficient immunoassay: Using electro-microchip, gold nanoparticle and silver enhancement. in Proceedings of the 33rd Annual Conference of the IEEE Industrial Electronics Society, IECON., 4459931, IECON Proceedings (Industrial Electronics Conference), pp. 2966-2969, 33rd Annual Conference of the IEEE Industrial Electronics Society, IECON, Taipei, Taiwan, 07-11-05. https://doi.org/10.1109/IECON.2007.4459931

A novel and efficient immunoassay : Using electro-microchip, gold nanoparticle and silver enhancement. / Huang, Keng Shiang; Chen, Wei Ting; Lee, Sheng Ji; Yeh, Chia Hsien; Chang, Tsung Chain; Lin, Hong-Ping; Lin, Yu-Cheng.

Proceedings of the 33rd Annual Conference of the IEEE Industrial Electronics Society, IECON. 2007. p. 2966-2969 4459931 (IECON Proceedings (Industrial Electronics Conference)).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

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Huang KS, Chen WT, Lee SJ, Yeh CH, Chang TC, Lin H-P et al. A novel and efficient immunoassay: Using electro-microchip, gold nanoparticle and silver enhancement. In Proceedings of the 33rd Annual Conference of the IEEE Industrial Electronics Society, IECON. 2007. p. 2966-2969. 4459931. (IECON Proceedings (Industrial Electronics Conference)). https://doi.org/10.1109/IECON.2007.4459931