TY - GEN
T1 - A novel and efficient immunoassay
T2 - 33rd Annual Conference of the IEEE Industrial Electronics Society, IECON
AU - Huang, Keng Shiang
AU - Chen, Wei Ting
AU - Lee, Sheng Ji
AU - Yeh, Chia Hsien
AU - Chang, Tsung Chain
AU - Lin, Hong Ping
AU - Lin, Yu Cheng
N1 - Copyright:
Copyright 2008 Elsevier B.V., All rights reserved.
PY - 2007
Y1 - 2007
N2 - This paper reports a novel immunoassay using an electro-microchip to detect the immuno-reaction signal, a gold nanoparticles (AuNPs) as a label of antigen or antibody and as a catalyst for silver precipitation, and the silver enhancement reaction to magnify the detection signal. The LCR meter instrument, a new detection method for immunoassay, is used to detect the electric single in a sandwich format. The change of the resistance to corroborate immuno-reaction is detected, amplified and analyzed to construct this new immunoassay. Different sizes of the electrodes are designed to discuss the relationship between gap size and sensitivity. In addition, the specificity, sensitivity and the detection limit of the electro-immunoassay are discussed. Using electro-microchip, nanoparticle and silver enhancement provided a new immunoassay method.
AB - This paper reports a novel immunoassay using an electro-microchip to detect the immuno-reaction signal, a gold nanoparticles (AuNPs) as a label of antigen or antibody and as a catalyst for silver precipitation, and the silver enhancement reaction to magnify the detection signal. The LCR meter instrument, a new detection method for immunoassay, is used to detect the electric single in a sandwich format. The change of the resistance to corroborate immuno-reaction is detected, amplified and analyzed to construct this new immunoassay. Different sizes of the electrodes are designed to discuss the relationship between gap size and sensitivity. In addition, the specificity, sensitivity and the detection limit of the electro-immunoassay are discussed. Using electro-microchip, nanoparticle and silver enhancement provided a new immunoassay method.
UR - http://www.scopus.com/inward/record.url?scp=49949104346&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=49949104346&partnerID=8YFLogxK
U2 - 10.1109/IECON.2007.4459931
DO - 10.1109/IECON.2007.4459931
M3 - Conference contribution
AN - SCOPUS:49949104346
SN - 1424407834
SN - 9781424407835
T3 - IECON Proceedings (Industrial Electronics Conference)
SP - 2966
EP - 2969
BT - Proceedings of the 33rd Annual Conference of the IEEE Industrial Electronics Society, IECON
Y2 - 5 November 2007 through 8 November 2007
ER -