A novel and efficient immunoassay: Using electro-microchip, gold nanoparticle and silver enhancement

Keng Shiang Huang; Wei Ting Chen; Sheng Ji Lee; Chia Hsien Yeh; Tsung Chain Chang; Hong Ping Lin; Yu Cheng Lin

doi:10.1109/IECON.2007.4459931

A novel and efficient immunoassay: Using electro-microchip, gold nanoparticle and silver enhancement

Keng Shiang Huang, Wei Ting Chen, Sheng Ji Lee, Chia Hsien Yeh, Tsung Chain Chang, Hong Ping Lin, Yu Cheng Lin

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

1 Citation (Scopus)

Abstract

This paper reports a novel immunoassay using an electro-microchip to detect the immuno-reaction signal, a gold nanoparticles (AuNPs) as a label of antigen or antibody and as a catalyst for silver precipitation, and the silver enhancement reaction to magnify the detection signal. The LCR meter instrument, a new detection method for immunoassay, is used to detect the electric single in a sandwich format. The change of the resistance to corroborate immuno-reaction is detected, amplified and analyzed to construct this new immunoassay. Different sizes of the electrodes are designed to discuss the relationship between gap size and sensitivity. In addition, the specificity, sensitivity and the detection limit of the electro-immunoassay are discussed. Using electro-microchip, nanoparticle and silver enhancement provided a new immunoassay method.

Original language	English
Title of host publication	Proceedings of the 33rd Annual Conference of the IEEE Industrial Electronics Society, IECON
Pages	2966-2969
Number of pages	4
DOIs	https://doi.org/10.1109/IECON.2007.4459931
Publication status	Published - 2007
Event	33rd Annual Conference of the IEEE Industrial Electronics Society, IECON - Taipei, Taiwan Duration: 2007 Nov 5 → 2007 Nov 8

Publication series

Name	IECON Proceedings (Industrial Electronics Conference)

Other

Other	33rd Annual Conference of the IEEE Industrial Electronics Society, IECON
Country	Taiwan
City	Taipei
Period	07-11-05 → 07-11-08

All Science Journal Classification (ASJC) codes

Control and Systems Engineering
Electrical and Electronic Engineering

Access to Document

10.1109/IECON.2007.4459931

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Huang, K. S., Chen, W. T., Lee, S. J., Yeh, C. H., Chang, T. C., Lin, H. P., & Lin, Y. C. (2007). A novel and efficient immunoassay: Using electro-microchip, gold nanoparticle and silver enhancement. In Proceedings of the 33rd Annual Conference of the IEEE Industrial Electronics Society, IECON (pp. 2966-2969). [4459931] (IECON Proceedings (Industrial Electronics Conference)). https://doi.org/10.1109/IECON.2007.4459931

Huang, Keng Shiang ; Chen, Wei Ting ; Lee, Sheng Ji ; Yeh, Chia Hsien ; Chang, Tsung Chain ; Lin, Hong Ping ; Lin, Yu Cheng. / A novel and efficient immunoassay : Using electro-microchip, gold nanoparticle and silver enhancement. Proceedings of the 33rd Annual Conference of the IEEE Industrial Electronics Society, IECON. 2007. pp. 2966-2969 (IECON Proceedings (Industrial Electronics Conference)).

@inproceedings{b1ed365a00aa4b899bc9a97ff360a17f,

title = "A novel and efficient immunoassay: Using electro-microchip, gold nanoparticle and silver enhancement",

abstract = "This paper reports a novel immunoassay using an electro-microchip to detect the immuno-reaction signal, a gold nanoparticles (AuNPs) as a label of antigen or antibody and as a catalyst for silver precipitation, and the silver enhancement reaction to magnify the detection signal. The LCR meter instrument, a new detection method for immunoassay, is used to detect the electric single in a sandwich format. The change of the resistance to corroborate immuno-reaction is detected, amplified and analyzed to construct this new immunoassay. Different sizes of the electrodes are designed to discuss the relationship between gap size and sensitivity. In addition, the specificity, sensitivity and the detection limit of the electro-immunoassay are discussed. Using electro-microchip, nanoparticle and silver enhancement provided a new immunoassay method.",

author = "Huang, {Keng Shiang} and Chen, {Wei Ting} and Lee, {Sheng Ji} and Yeh, {Chia Hsien} and Chang, {Tsung Chain} and Lin, {Hong Ping} and Lin, {Yu Cheng}",

year = "2007",

doi = "10.1109/IECON.2007.4459931",

language = "English",

isbn = "1424407834",

series = "IECON Proceedings (Industrial Electronics Conference)",

pages = "2966--2969",

booktitle = "Proceedings of the 33rd Annual Conference of the IEEE Industrial Electronics Society, IECON",

}

Huang, KS, Chen, WT, Lee, SJ, Yeh, CH, Chang, TC, Lin, HP & Lin, YC 2007, A novel and efficient immunoassay: Using electro-microchip, gold nanoparticle and silver enhancement. in Proceedings of the 33rd Annual Conference of the IEEE Industrial Electronics Society, IECON., 4459931, IECON Proceedings (Industrial Electronics Conference), pp. 2966-2969, 33rd Annual Conference of the IEEE Industrial Electronics Society, IECON, Taipei, Taiwan, 07-11-05. https://doi.org/10.1109/IECON.2007.4459931

A novel and efficient immunoassay : Using electro-microchip, gold nanoparticle and silver enhancement. / Huang, Keng Shiang; Chen, Wei Ting; Lee, Sheng Ji; Yeh, Chia Hsien; Chang, Tsung Chain; Lin, Hong Ping ; Lin, Yu Cheng.

Proceedings of the 33rd Annual Conference of the IEEE Industrial Electronics Society, IECON. 2007. p. 2966-2969 4459931 (IECON Proceedings (Industrial Electronics Conference)).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

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AU - Huang, Keng Shiang

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AU - Chang, Tsung Chain

AU - Lin, Hong Ping

AU - Lin, Yu Cheng

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AB - This paper reports a novel immunoassay using an electro-microchip to detect the immuno-reaction signal, a gold nanoparticles (AuNPs) as a label of antigen or antibody and as a catalyst for silver precipitation, and the silver enhancement reaction to magnify the detection signal. The LCR meter instrument, a new detection method for immunoassay, is used to detect the electric single in a sandwich format. The change of the resistance to corroborate immuno-reaction is detected, amplified and analyzed to construct this new immunoassay. Different sizes of the electrodes are designed to discuss the relationship between gap size and sensitivity. In addition, the specificity, sensitivity and the detection limit of the electro-immunoassay are discussed. Using electro-microchip, nanoparticle and silver enhancement provided a new immunoassay method.

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Huang KS, Chen WT, Lee SJ, Yeh CH, Chang TC, Lin HP et al. A novel and efficient immunoassay: Using electro-microchip, gold nanoparticle and silver enhancement. In Proceedings of the 33rd Annual Conference of the IEEE Industrial Electronics Society, IECON. 2007. p. 2966-2969. 4459931. (IECON Proceedings (Industrial Electronics Conference)). https://doi.org/10.1109/IECON.2007.4459931