Abstract
The present study explores a method for scaling-up a fermentation system. The method consists of two parts. The first part investigates the effects of the environmental state variables on cultivation. If a wide range of the state variables has no significant effect on yield or productivity, scaling-up is not an issue in the fermentation system. If only a small range of the state variables has no substantial impact on cultivation, then pulse or periodical change of the state variables would be employed to investigate the effects of the change on cultivation. To illustrate the proposed method of scale-up, Bacillus thuringiensis was cultivated for thuringiensin production with the pH value as the environmental state variable. Different pH values at 7.0 and 8.4 had a significant effect on both cell growth and thuringiensin production. Variation of pH value for a short period of time did not have a substantial effect on either cell growth or thuringiensin production.
| Original language | English |
|---|---|
| Pages (from-to) | 123-130 |
| Number of pages | 8 |
| Journal | Biochemical Engineering Journal |
| Volume | 11 |
| Issue number | 2-3 |
| DOIs | |
| Publication status | Published - 2002 Sept |
All Science Journal Classification (ASJC) codes
- Biotechnology
- Environmental Engineering
- Bioengineering
- Biomedical Engineering