A novel dielectrophoresis-based microfluidic chip for antibody-free isolation of circulating tumor cells from blood

I. Fang Cheng, Tzu Ying Chen, Yu De Lin, Wei Lung Huang, Chien Wei Liu, Wu-Chou Su

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

An antibody-free approach for high purity and high throughput isolation of circulating tumor cells (CTCs) from human blood in a microfluidic chip is presented. High throughput continuous CTC isolation (>1.2 ml/hr) is achieved with a sustained dielectrophoresis (DEP) particle force normal to the continuous through-flow. The design allows continuous fractionation of cells into different downstream sub-channels based on the differences in their different critical DEP strengths/mobility on both sides of the cross-over. An enrichment factor of ∼105 and a recovery rate of ∼80% from a 0.001% CTC sample is achieved at a high throughput of 1.2 ml/hr. Compared to conventional physical methods, such as immunomagnetics-based methods and microfluidic biomarker-based methods, the isolation purity, recovery rate, throughput and viability of the proposed antibody-free chip demonstrated excellent capabilities, especially with regard to high enrichment at a high flow rate.

Original languageEnglish
Title of host publicationIEEE-NANO 2015 - 15th International Conference on Nanotechnology
PublisherInstitute of Electrical and Electronics Engineers Inc.
Pages1525-1527
Number of pages3
ISBN (Electronic)9781467381550
DOIs
Publication statusPublished - 2015 Jan 1
Event15th IEEE International Conference on Nanotechnology, IEEE-NANO 2015 - Rome, Italy
Duration: 2015 Jul 272015 Jul 30

Publication series

NameIEEE-NANO 2015 - 15th International Conference on Nanotechnology

Other

Other15th IEEE International Conference on Nanotechnology, IEEE-NANO 2015
CountryItaly
CityRome
Period15-07-2715-07-30

Fingerprint

Electrophoresis
Microfluidics
Antibodies
Tumors
Blood
Cells
Throughput
Recovery
Biomarkers
Fractionation
Flow rate

All Science Journal Classification (ASJC) codes

  • Process Chemistry and Technology
  • Electrical and Electronic Engineering
  • Ceramics and Composites
  • Electronic, Optical and Magnetic Materials
  • Surfaces, Coatings and Films

Cite this

Cheng, I. F., Chen, T. Y., Lin, Y. D., Huang, W. L., Liu, C. W., & Su, W-C. (2015). A novel dielectrophoresis-based microfluidic chip for antibody-free isolation of circulating tumor cells from blood. In IEEE-NANO 2015 - 15th International Conference on Nanotechnology (pp. 1525-1527). [7388934] (IEEE-NANO 2015 - 15th International Conference on Nanotechnology). Institute of Electrical and Electronics Engineers Inc.. https://doi.org/10.1109/NANO.2015.7388934
Cheng, I. Fang ; Chen, Tzu Ying ; Lin, Yu De ; Huang, Wei Lung ; Liu, Chien Wei ; Su, Wu-Chou. / A novel dielectrophoresis-based microfluidic chip for antibody-free isolation of circulating tumor cells from blood. IEEE-NANO 2015 - 15th International Conference on Nanotechnology. Institute of Electrical and Electronics Engineers Inc., 2015. pp. 1525-1527 (IEEE-NANO 2015 - 15th International Conference on Nanotechnology).
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Cheng, IF, Chen, TY, Lin, YD, Huang, WL, Liu, CW & Su, W-C 2015, A novel dielectrophoresis-based microfluidic chip for antibody-free isolation of circulating tumor cells from blood. in IEEE-NANO 2015 - 15th International Conference on Nanotechnology., 7388934, IEEE-NANO 2015 - 15th International Conference on Nanotechnology, Institute of Electrical and Electronics Engineers Inc., pp. 1525-1527, 15th IEEE International Conference on Nanotechnology, IEEE-NANO 2015, Rome, Italy, 15-07-27. https://doi.org/10.1109/NANO.2015.7388934

A novel dielectrophoresis-based microfluidic chip for antibody-free isolation of circulating tumor cells from blood. / Cheng, I. Fang; Chen, Tzu Ying; Lin, Yu De; Huang, Wei Lung; Liu, Chien Wei; Su, Wu-Chou.

IEEE-NANO 2015 - 15th International Conference on Nanotechnology. Institute of Electrical and Electronics Engineers Inc., 2015. p. 1525-1527 7388934 (IEEE-NANO 2015 - 15th International Conference on Nanotechnology).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

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Cheng IF, Chen TY, Lin YD, Huang WL, Liu CW, Su W-C. A novel dielectrophoresis-based microfluidic chip for antibody-free isolation of circulating tumor cells from blood. In IEEE-NANO 2015 - 15th International Conference on Nanotechnology. Institute of Electrical and Electronics Engineers Inc. 2015. p. 1525-1527. 7388934. (IEEE-NANO 2015 - 15th International Conference on Nanotechnology). https://doi.org/10.1109/NANO.2015.7388934