TY - GEN
T1 - A novel DNA hybridization assay using gold-streptavidin, silver enhancement and a flatbed scanner
AU - Yeh, Chia Hsien
AU - Chang, Yu Huai
AU - Lin, Hong-Ping
AU - Chang, Tsung Chain
AU - Lin, Yu-Cheng
PY - 2009/1/1
Y1 - 2009/1/1
N2 - This paper reports a novel DNA hybridization detection method using gold-streptavidin (Au-streptavidin) conjugates as a reporter, the silver enhancement reaction to magnify the detection signal, and a flatbed scanner to scan and detect the hybridization signal. The detection limit of DNA concentration is 8.25 ng/mL and we also differentiate the specific DNA samples. In addition, the time of the whole detection was 18 minutes, which is shorter than the conventional enzyme staining (approximately 60 minutes).
AB - This paper reports a novel DNA hybridization detection method using gold-streptavidin (Au-streptavidin) conjugates as a reporter, the silver enhancement reaction to magnify the detection signal, and a flatbed scanner to scan and detect the hybridization signal. The detection limit of DNA concentration is 8.25 ng/mL and we also differentiate the specific DNA samples. In addition, the time of the whole detection was 18 minutes, which is shorter than the conventional enzyme staining (approximately 60 minutes).
UR - http://www.scopus.com/inward/record.url?scp=84901798832&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84901798832&partnerID=8YFLogxK
M3 - Conference contribution
AN - SCOPUS:84901798832
SN - 9780979806421
T3 - Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences
SP - 948
EP - 951
BT - Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences
PB - Chemical and Biological Microsystems Society
T2 - 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2009
Y2 - 1 November 2009 through 5 November 2009
ER -