A novel endo-glucanase from the thermophilic bacterium Geobacillus sp. 70PC53 with high activity and stability over a broad range of temperatures

I. Son Ng, Chen Wei Li, Yi Fang Yeh, Po Ting Chen, Jiun Ly Chir, Chin Hua Ma, Su May Yu, Tuan Hua David Ho, Chii Gong Tong

Research output: Contribution to journalArticlepeer-review

50 Citations (Scopus)

Abstract

A thermophilic Geobacillus bacterium secreting high activity of endo-glucanase (EC 3.2.1.4) was isolated from rice straw compost supplemented with pig manure. A full-length gene of 1,104 bp, celA, encoding this glycosyl hydrolase family 5 endo-glucanase of 368 amino acids was isolated. No related gene from Geobacillus has been reported previously. The recombinant CelA expressed in Escherichia coli had an optimal activity at 65°C and pH 5.0, and it exhibited tenfold greater specific activity than the commercially available Trichoderma reesei endo-glucanase. CelA displayed activity over a broad temperature range from 45 to 75°C and was a thermostable enzyme with 90% activity retained after heating at 65°C for 6 h. Interestingly, CelA activity could be enhanced by 100% in the presence of 2 mM MnSO4. CelA had high specific activity over β-d-glucan from barley and Lichenan, making it a potentially useful enzyme in biofuel and food industries.

Original languageEnglish
Pages (from-to)425-435
Number of pages11
JournalExtremophiles
Volume13
Issue number3
DOIs
Publication statusPublished - 2009 May 1

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Molecular Medicine

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